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Cytoprotective Effect of Makgeolli Lees on Paraquat Induced Oxidative Stress in A549 Cells via Activation of NRF2 and Antioxidant Genes.
J Microbiol Biotechnol. 2016 Feb; 26(2):277-86.JM

Abstract

Makgeolli lees (ML) has several physiological effects such as antioxidant, antidiabetic, and anticancer properties, but its biological functions have not been determined definitively. Here, we tested whether ML has a cytoprotective effect on paraquat (PQ)-induced oxidative stress in the human lung carcinoma cell line A549. At 0.1 mg/ml ML, viability of PQ-exposed A549 cells was restored by 12.4%, 18.5%, and 48.6% after 24, 48, and 72 h, respectively. ML also reduced production of the intracellular reactive oxygen species (ROS) that were generated by PQ treatment. Further experiments revealed that ML treatment enhanced the expression and nuclear translocation of nuclear factor erythroid 2-related factor 2 (NRF2) as well as ARE-GFP reporter activity. ML treatment also effectively increased the expression of NRF2's target genes NAD(P)H dehydrogenase quinone 1 (NQO1) and heme oxygenase 1 (HO-1). Moreover, we found that expression of cytoprotective genes, including glutathione peroxidases (GPXs), superoxide dismutase (SOD1), catalase (CAT), peroxiredoxin 3 (PRDX3), and peroxiredoxin 4 (PRDX4), was greatly enhanced by treatment with ML during PQ exposure. Taken together, the data suggest that treatment of PQ-exposed A549 cells with ML ameliorates cytotoxicity through induction of NRF2 expression and its target genes HO-1, NQO1, and other antioxidant genes. Thus, ML may serve as a functional food applicable to ROS-mediated human diseases.

Authors+Show Affiliations

Department of Microbiology, College of Medicine, Soonchunhyang University, Cheonan 31151, Republic of Korea.Department of Microbiology, College of Medicine, Soonchunhyang University, Cheonan 31151, Republic of Korea. Institute of Tissue Engineering, College of Medicine, Soonchunhyang University, Cheonan 31151, Republic of Korea.Department of Microbiology, College of Medicine, Soonchunhyang University, Cheonan 31151, Republic of Korea. Institute of Tissue Engineering, College of Medicine, Soonchunhyang University, Cheonan 31151, Republic of Korea.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26597533

Citation

Jeon, Miso, et al. "Cytoprotective Effect of Makgeolli Lees On Paraquat Induced Oxidative Stress in A549 Cells Via Activation of NRF2 and Antioxidant Genes." Journal of Microbiology and Biotechnology, vol. 26, no. 2, 2016, pp. 277-86.
Jeon M, Rahman N, Kim YS. Cytoprotective Effect of Makgeolli Lees on Paraquat Induced Oxidative Stress in A549 Cells via Activation of NRF2 and Antioxidant Genes. J Microbiol Biotechnol. 2016;26(2):277-86.
Jeon, M., Rahman, N., & Kim, Y. S. (2016). Cytoprotective Effect of Makgeolli Lees on Paraquat Induced Oxidative Stress in A549 Cells via Activation of NRF2 and Antioxidant Genes. Journal of Microbiology and Biotechnology, 26(2), 277-86. https://doi.org/10.4014/jmb.1510.10093
Jeon M, Rahman N, Kim YS. Cytoprotective Effect of Makgeolli Lees On Paraquat Induced Oxidative Stress in A549 Cells Via Activation of NRF2 and Antioxidant Genes. J Microbiol Biotechnol. 2016;26(2):277-86. PubMed PMID: 26597533.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cytoprotective Effect of Makgeolli Lees on Paraquat Induced Oxidative Stress in A549 Cells via Activation of NRF2 and Antioxidant Genes. AU - Jeon,Miso, AU - Rahman,Naimur, AU - Kim,Yong-Sik, PY - 2015/11/25/entrez PY - 2015/11/26/pubmed PY - 2016/11/12/medline KW - A549 KW - Antioxidant response element KW - Makgeolli lees KW - NRF2 KW - Oxidative stress KW - Paraquat SP - 277 EP - 86 JF - Journal of microbiology and biotechnology JO - J Microbiol Biotechnol VL - 26 IS - 2 N2 - Makgeolli lees (ML) has several physiological effects such as antioxidant, antidiabetic, and anticancer properties, but its biological functions have not been determined definitively. Here, we tested whether ML has a cytoprotective effect on paraquat (PQ)-induced oxidative stress in the human lung carcinoma cell line A549. At 0.1 mg/ml ML, viability of PQ-exposed A549 cells was restored by 12.4%, 18.5%, and 48.6% after 24, 48, and 72 h, respectively. ML also reduced production of the intracellular reactive oxygen species (ROS) that were generated by PQ treatment. Further experiments revealed that ML treatment enhanced the expression and nuclear translocation of nuclear factor erythroid 2-related factor 2 (NRF2) as well as ARE-GFP reporter activity. ML treatment also effectively increased the expression of NRF2's target genes NAD(P)H dehydrogenase quinone 1 (NQO1) and heme oxygenase 1 (HO-1). Moreover, we found that expression of cytoprotective genes, including glutathione peroxidases (GPXs), superoxide dismutase (SOD1), catalase (CAT), peroxiredoxin 3 (PRDX3), and peroxiredoxin 4 (PRDX4), was greatly enhanced by treatment with ML during PQ exposure. Taken together, the data suggest that treatment of PQ-exposed A549 cells with ML ameliorates cytotoxicity through induction of NRF2 expression and its target genes HO-1, NQO1, and other antioxidant genes. Thus, ML may serve as a functional food applicable to ROS-mediated human diseases. SN - 1738-8872 UR - https://www.unboundmedicine.com/medline/citation/26597533/Cytoprotective_Effect_of_Makgeolli_Lees_on_Paraquat_Induced_Oxidative_Stress_in_A549_Cells_via_Activation_of_NRF2_and_Antioxidant_Genes_ L2 - http://www.jmb.or.kr/journal/view.html?doi=10.4014/jmb.1510.10093 DB - PRIME DP - Unbound Medicine ER -