Highly Sensitive and Selective Photoelectrochemical Biosensor for Hg(2+) Detection Based on Dual Signal Amplification by Exciton Energy Transfer Coupled with Sensitization Effect.
Anal Chem. 2015 Dec 15; 87(24):12340-7.AC

Abstract

A highly sensitive and selective photoelectrochemical (PEC) biosensor for Hg(2+) detection was developed on the basis of the synergistic effect of exciton energy transfer (EET) between CdS quantum dots (QDs) and Au nanoparticles (NPs) coupled with sensitization of rhodamine 123 (Rh123) for signal amplification. First, the TiO2/CdS hybrid structure obtained by depositing CdS QDs on TiO2 film was employed as a matrix for immobilizing probe DNA (pDNA). Next, Rh123 was introduced into the pDNA terminal, and then Au NP labeled target DNA (Au-tDNA) was hybridized with pDNA to form a rod-like double helix structure. The detection of Hg(2+) was based on a conformational change of the pDNA after incubating with Hg(2+). In the absence of Hg(2+), Rh123 was located away from the electrode surface due to the DNA hybridization, leading to inhibition of the sensitization effect, and meanwhile, the occurrence of EET between CdS QDs and Au NPs resulted in a photocurrent decrease. However, after incubating with Hg(2+), the rod-like double helix was disrupted, and the energy transfer was broken. In this case, the photocurrent recovered, and meanwhile, the folded pDNA made the labeled Rh123 move closer to the electrode surface, leading to the formation of the sensitization structure, which evidently increased the photocurrent intensity. The sensitivity of the biosensor for Hg(2+) detection was greatly enhanced for the dual signal amplification strategy. The linear range was 10 fM to 200 nM, with a detection limit of 3.3 fM. This biosensor provides a promising new platform for detecting various heavy metal ions at ultralow levels.

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Authors+Show Affiliations

Zhao M

School of Chemical Engineering, Anhui University of Science and Technology , Huainan 232001, People's Republic of China.

Fan GC

State Key Laboratory of Analytical Chemistry for Life Science, Collaborative Innovation Center of Chemistry for Life Sciences, School of Chemistry and Chemical Engineering, Nanjing University , Nanjing 210093, People's Republic of China.

Chen JJ

Shi JJ

School of Chemical Engineering, Anhui University of Science and Technology , Huainan 232001, People's Republic of China. State Key Laboratory of Analytical Chemistry for Life Science, Collaborative Innovation Center of Chemistry for Life Sciences, School of Chemistry and Chemical Engineering, Nanjing University , Nanjing 210093, People's Republic of China.

Zhu JJ

MeSH

Biosensing TechniquesCadmium CompoundsDNAElectrochemical TechniquesEnergy TransferGoldMercuryMetal NanoparticlesPhotochemical ProcessesQuantum DotsRhodamine 123Sulfides

Pub Type(s)

Journal Article

Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26599580

Citation

Zhao, Ming, et al. "Highly Sensitive and Selective Photoelectrochemical Biosensor for Hg(2+) Detection Based On Dual Signal Amplification By Exciton Energy Transfer Coupled With Sensitization Effect." Analytical Chemistry, vol. 87, no. 24, 2015, pp. 12340-7.

Zhao M, Fan GC, Chen JJ, et al. Highly Sensitive and Selective Photoelectrochemical Biosensor for Hg(2+) Detection Based on Dual Signal Amplification by Exciton Energy Transfer Coupled with Sensitization Effect. Anal Chem. 2015;87(24):12340-7.

Zhao, M., Fan, G. C., Chen, J. J., Shi, J. J., & Zhu, J. J. (2015). Highly Sensitive and Selective Photoelectrochemical Biosensor for Hg(2+) Detection Based on Dual Signal Amplification by Exciton Energy Transfer Coupled with Sensitization Effect. Analytical Chemistry, 87(24), 12340-7. https://doi.org/10.1021/acs.analchem.5b03721

Zhao M, et al. Highly Sensitive and Selective Photoelectrochemical Biosensor for Hg(2+) Detection Based On Dual Signal Amplification By Exciton Energy Transfer Coupled With Sensitization Effect. Anal Chem. 2015 Dec 15;87(24):12340-7. PubMed PMID: 26599580.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Highly Sensitive and Selective Photoelectrochemical Biosensor for Hg(2+) Detection Based on Dual Signal Amplification by Exciton Energy Transfer Coupled with Sensitization Effect. AU - Zhao,Ming, AU - Fan,Gao-Chao, AU - Chen,Jing-Jia, AU - Shi,Jian-Jun, AU - Zhu,Jun-Jie, Y1 - 2015/12/04/ PY - 2015/11/25/entrez PY - 2015/11/26/pubmed PY - 2016/7/12/medline SP - 12340 EP - 7 JF - Analytical chemistry JO - Anal Chem VL - 87 IS - 24 N2 - A highly sensitive and selective photoelectrochemical (PEC) biosensor for Hg(2+) detection was developed on the basis of the synergistic effect of exciton energy transfer (EET) between CdS quantum dots (QDs) and Au nanoparticles (NPs) coupled with sensitization of rhodamine 123 (Rh123) for signal amplification. First, the TiO2/CdS hybrid structure obtained by depositing CdS QDs on TiO2 film was employed as a matrix for immobilizing probe DNA (pDNA). Next, Rh123 was introduced into the pDNA terminal, and then Au NP labeled target DNA (Au-tDNA) was hybridized with pDNA to form a rod-like double helix structure. The detection of Hg(2+) was based on a conformational change of the pDNA after incubating with Hg(2+). In the absence of Hg(2+), Rh123 was located away from the electrode surface due to the DNA hybridization, leading to inhibition of the sensitization effect, and meanwhile, the occurrence of EET between CdS QDs and Au NPs resulted in a photocurrent decrease. However, after incubating with Hg(2+), the rod-like double helix was disrupted, and the energy transfer was broken. In this case, the photocurrent recovered, and meanwhile, the folded pDNA made the labeled Rh123 move closer to the electrode surface, leading to the formation of the sensitization structure, which evidently increased the photocurrent intensity. The sensitivity of the biosensor for Hg(2+) detection was greatly enhanced for the dual signal amplification strategy. The linear range was 10 fM to 200 nM, with a detection limit of 3.3 fM. This biosensor provides a promising new platform for detecting various heavy metal ions at ultralow levels. SN - 1520-6882 UR - https://www.unboundmedicine.com/medline/citation/26599580/Highly_Sensitive_and_Selective_Photoelectrochemical_Biosensor_for_Hg_2+__Detection_Based_on_Dual_Signal_Amplification_by_Exciton_Energy_Transfer_Coupled_with_Sensitization_Effect_ DB - PRIME DP - Unbound Medicine ER -

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Highly Sensitive and Selective Photoelectrochemical Biosensor for Hg(2+) Detection Based on Dual Signal Amplification by Exciton Energy Transfer Coupled with Sensitization Effect.Anal Chem. 2015 Dec 15; 87(24):12340-7.AC