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Diversity of Multi-Drug Resistant Avian Pathogenic Escherichia coli (APEC) Causing Outbreaks of Colibacillosis in Broilers during 2012 in Spain.
PLoS One. 2015; 10(11):e0143191.Plos

Abstract

Avian pathogenic Escherichia coli (APEC) are the major cause of colibacillosis in poultry production. In this study, a total of 22 E. coli isolated from colibacillosis field cases and 10 avian faecal E. coli (AFEC) were analysed. All strains were characterised phenotypically by susceptibility testing and molecular typing methods such as pulsed-field gel electrophoresis (PFGE) and multi-locus sequence typing (MLST). The presence of 29 virulence genes associated to APEC and human extraintestinal pathogenic E. coli (ExPEC) was also evaluated. For cephalosporin resistant isolates, cephalosporin resistance genes, plasmid location and replicon typing was assessed. Avian isolates belonged to 26 O:H serotypes and 24 sequence types. Out of 22 APEC isolates, 91% contained the virulence genes predictors of APEC; iutA, hlyF, iss, iroN and ompT. Of all strains, 34% were considered ExPEC. PFGE analysis demonstrated a high degree of genetic polymorphism. All strains were multi-resistant, including those isolated from healthy animals. Eleven strains were resistant to cephalosporins; six contained blaCTX-M-14, two blaSHV-12, two blaCMY-2 and one blaSHV-2. Two strains harboured qnrA, and two qnrA together with aac(6')-Ib-cr. Additionally, the emergent clone O25b:H4-B2-ST131 was isolated from a healthy animal which harboured blaCMY-2 and qnrS genes. Cephalosporin resistant genes were mainly associated to the presence of IncK replicons. This study demonstrates a very diverse population of multi-drug resistant E. coli containing a high number of virulent genes. The E. coli population among broilers is a reservoir of resistance and virulence-associated genes that could be transmitted into the community through the food chain. More epidemiological studies are necessary to identify clonal groups and resistance mechanisms with potential relevance to public health.

Authors+Show Affiliations

Centre de Recerca en Sanitat Animal (CReSA)-Institut de Recerca i Tecnologia Agroalimentàries (IRTA), Campus UAB, Barcelona, Spain.Centre de Recerca en Sanitat Animal (CReSA)-Institut de Recerca i Tecnologia Agroalimentàries (IRTA), Campus UAB, Barcelona, Spain.Centre de Recerca en Sanitat Animal (CReSA)-Institut de Recerca i Tecnologia Agroalimentàries (IRTA), Campus UAB, Barcelona, Spain.Centre de Recerca en Sanitat Animal (CReSA)-Institut de Recerca i Tecnologia Agroalimentàries (IRTA), Campus UAB, Barcelona, Spain.Centre de Recerca en Sanitat Animal (CReSA)-Institut de Recerca i Tecnologia Agroalimentàries (IRTA), Campus UAB, Barcelona, Spain. Departament de Sanitat i Anatomia Animals, Universitat Autònoma de Barcelona, Bellaterra (Cerdanyola del Vallés), Spain.Laboratorio de Referencia de E. coli, Departamento de Microbioloxía e Parasitoloxía, Facultade de Veterinaria, Universidade de Santiago de Compostela, Lugo, Spain.Laboratorio de Referencia de E. coli, Departamento de Microbioloxía e Parasitoloxía, Facultade de Veterinaria, Universidade de Santiago de Compostela, Lugo, Spain.Laboratorio de Referencia de E. coli, Departamento de Microbioloxía e Parasitoloxía, Facultade de Veterinaria, Universidade de Santiago de Compostela, Lugo, Spain.Laboratorio de Referencia de E. coli, Departamento de Microbioloxía e Parasitoloxía, Facultade de Veterinaria, Universidade de Santiago de Compostela, Lugo, Spain.Servei de Microbiologia, Hospital Vall d'Hebron, Universitat Autònoma de Barcelona, Barcelona, Spain.Servei de Microbiologia, Hospital Vall d'Hebron, Universitat Autònoma de Barcelona, Barcelona, Spain.Centre de Recerca en Sanitat Animal (CReSA)-Institut de Recerca i Tecnologia Agroalimentàries (IRTA), Campus UAB, Barcelona, Spain.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26600205

Citation

Solà-Ginés, Marc, et al. "Diversity of Multi-Drug Resistant Avian Pathogenic Escherichia Coli (APEC) Causing Outbreaks of Colibacillosis in Broilers During 2012 in Spain." PloS One, vol. 10, no. 11, 2015, pp. e0143191.
Solà-Ginés M, Cameron-Veas K, Badiola I, et al. Diversity of Multi-Drug Resistant Avian Pathogenic Escherichia coli (APEC) Causing Outbreaks of Colibacillosis in Broilers during 2012 in Spain. PLoS One. 2015;10(11):e0143191.
Solà-Ginés, M., Cameron-Veas, K., Badiola, I., Dolz, R., Majó, N., Dahbi, G., Viso, S., Mora, A., Blanco, J., Piedra-Carrasco, N., González-López, J. J., & Migura-Garcia, L. (2015). Diversity of Multi-Drug Resistant Avian Pathogenic Escherichia coli (APEC) Causing Outbreaks of Colibacillosis in Broilers during 2012 in Spain. PloS One, 10(11), e0143191. https://doi.org/10.1371/journal.pone.0143191
Solà-Ginés M, et al. Diversity of Multi-Drug Resistant Avian Pathogenic Escherichia Coli (APEC) Causing Outbreaks of Colibacillosis in Broilers During 2012 in Spain. PLoS One. 2015;10(11):e0143191. PubMed PMID: 26600205.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Diversity of Multi-Drug Resistant Avian Pathogenic Escherichia coli (APEC) Causing Outbreaks of Colibacillosis in Broilers during 2012 in Spain. AU - Solà-Ginés,Marc, AU - Cameron-Veas,Karla, AU - Badiola,Ignacio, AU - Dolz,Roser, AU - Majó,Natalia, AU - Dahbi,Ghizlane, AU - Viso,Susana, AU - Mora,Azucena, AU - Blanco,Jorge, AU - Piedra-Carrasco,Nuria, AU - González-López,Juan José, AU - Migura-Garcia,Lourdes, Y1 - 2015/11/23/ PY - 2015/06/03/received PY - 2015/11/01/accepted PY - 2015/11/25/entrez PY - 2015/11/26/pubmed PY - 2016/6/21/medline SP - e0143191 EP - e0143191 JF - PloS one JO - PLoS One VL - 10 IS - 11 N2 - Avian pathogenic Escherichia coli (APEC) are the major cause of colibacillosis in poultry production. In this study, a total of 22 E. coli isolated from colibacillosis field cases and 10 avian faecal E. coli (AFEC) were analysed. All strains were characterised phenotypically by susceptibility testing and molecular typing methods such as pulsed-field gel electrophoresis (PFGE) and multi-locus sequence typing (MLST). The presence of 29 virulence genes associated to APEC and human extraintestinal pathogenic E. coli (ExPEC) was also evaluated. For cephalosporin resistant isolates, cephalosporin resistance genes, plasmid location and replicon typing was assessed. Avian isolates belonged to 26 O:H serotypes and 24 sequence types. Out of 22 APEC isolates, 91% contained the virulence genes predictors of APEC; iutA, hlyF, iss, iroN and ompT. Of all strains, 34% were considered ExPEC. PFGE analysis demonstrated a high degree of genetic polymorphism. All strains were multi-resistant, including those isolated from healthy animals. Eleven strains were resistant to cephalosporins; six contained blaCTX-M-14, two blaSHV-12, two blaCMY-2 and one blaSHV-2. Two strains harboured qnrA, and two qnrA together with aac(6')-Ib-cr. Additionally, the emergent clone O25b:H4-B2-ST131 was isolated from a healthy animal which harboured blaCMY-2 and qnrS genes. Cephalosporin resistant genes were mainly associated to the presence of IncK replicons. This study demonstrates a very diverse population of multi-drug resistant E. coli containing a high number of virulent genes. The E. coli population among broilers is a reservoir of resistance and virulence-associated genes that could be transmitted into the community through the food chain. More epidemiological studies are necessary to identify clonal groups and resistance mechanisms with potential relevance to public health. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/26600205/Diversity_of_Multi_Drug_Resistant_Avian_Pathogenic_Escherichia_coli__APEC__Causing_Outbreaks_of_Colibacillosis_in_Broilers_during_2012_in_Spain_ L2 - https://dx.plos.org/10.1371/journal.pone.0143191 DB - PRIME DP - Unbound Medicine ER -