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Diverse Burkholderia Species Isolated from Soils in the Southern United States with No Evidence of B. pseudomallei.
PLoS One. 2015; 10(11):e0143254.Plos

Abstract

The global distribution of the soil-dwelling bacterium Burkholderia pseudomallei, causative agent of melioidosis, is poorly understood. We used established culturing methods developed for B. pseudomallei to isolate Burkholderia species from soil collected at 18 sampling sites in three states in the southern United States (Arizona (n = 4), Florida (n = 7), and Louisiana (n = 7)). Using multi-locus sequence typing (MLST) of seven genes, we identified 35 Burkholderia isolates from these soil samples. All species belonged to the B. cepacia complex (Bcc), including B. cenocepacia, B. cepacia, B. contaminans, B. diffusa, B. metallica, B. seminalis, B. vietnamiensis and two unnamed members of the Bcc. The MLST analysis provided a high level of resolution among and within these species. Despite previous clinical cases within the U.S. involving B. pseudomallei and its close phylogenetic relatives, we did not isolate any of these taxa. The Bcc contains a number of opportunistic pathogens that cause infections in cystic fibrosis patients. Interestingly, we found that B. vietnamiensis was present in soil from all three states, suggesting it may be a common component in southern U.S. soils. Most of the Burkholderia isolates collected in this study were from Florida (30/35; 86%), which may be due to the combination of relatively moist, sandy, and acidic soils found there compared to the other two states. We also investigated one MLST gene, recA, for its ability to identify species within Burkholderia. A 365bp fragment of recA recovered nearly the same species-level identification as MLST, thus demonstrating its cost effective utility when conducting environmental surveys for Burkholderia. Although we did not find B. pseudomallei, our findings document that other diverse Burkholderia species are present in soils in the southern United States.

Authors+Show Affiliations

Center for Microbial Genetics and Genomics, Northern Arizona University, 1395 S Knoles Drive, Flagstaff, AZ 86011, United States of America.Center for Microbial Genetics and Genomics, Northern Arizona University, 1395 S Knoles Drive, Flagstaff, AZ 86011, United States of America.Center for Microbial Genetics and Genomics, Northern Arizona University, 1395 S Knoles Drive, Flagstaff, AZ 86011, United States of America.Center for Microbial Genetics and Genomics, Northern Arizona University, 1395 S Knoles Drive, Flagstaff, AZ 86011, United States of America.Menzies School of Health Research, Casuarina, NT, Australia.Menzies School of Health Research, Casuarina, NT, Australia.Center for Microbial Genetics and Genomics, Northern Arizona University, 1395 S Knoles Drive, Flagstaff, AZ 86011, United States of America.Translational Genomics Research Institute, Flagstaff, AZ 86001, United States of America.Translational Genomics Research Institute, Flagstaff, AZ 86001, United States of America.Center for Microbial Genetics and Genomics, Northern Arizona University, 1395 S Knoles Drive, Flagstaff, AZ 86011, United States of America. Translational Genomics Research Institute, Flagstaff, AZ 86001, United States of America.Menzies School of Health Research, Casuarina, NT, Australia.Center for Microbial Genetics and Genomics, Northern Arizona University, 1395 S Knoles Drive, Flagstaff, AZ 86011, United States of America.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26600238

Citation

Hall, Carina M., et al. "Diverse Burkholderia Species Isolated From Soils in the Southern United States With No Evidence of B. Pseudomallei." PloS One, vol. 10, no. 11, 2015, pp. e0143254.
Hall CM, Busch JD, Shippy K, et al. Diverse Burkholderia Species Isolated from Soils in the Southern United States with No Evidence of B. pseudomallei. PLoS One. 2015;10(11):e0143254.
Hall, C. M., Busch, J. D., Shippy, K., Allender, C. J., Kaestli, M., Mayo, M., Sahl, J. W., Schupp, J. M., Colman, R. E., Keim, P., Currie, B. J., & Wagner, D. M. (2015). Diverse Burkholderia Species Isolated from Soils in the Southern United States with No Evidence of B. pseudomallei. PloS One, 10(11), e0143254. https://doi.org/10.1371/journal.pone.0143254
Hall CM, et al. Diverse Burkholderia Species Isolated From Soils in the Southern United States With No Evidence of B. Pseudomallei. PLoS One. 2015;10(11):e0143254. PubMed PMID: 26600238.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Diverse Burkholderia Species Isolated from Soils in the Southern United States with No Evidence of B. pseudomallei. AU - Hall,Carina M, AU - Busch,Joseph D, AU - Shippy,Kenzie, AU - Allender,Christopher J, AU - Kaestli,Mirjam, AU - Mayo,Mark, AU - Sahl,Jason W, AU - Schupp,James M, AU - Colman,Rebecca E, AU - Keim,Paul, AU - Currie,Bart J, AU - Wagner,David M, Y1 - 2015/11/23/ PY - 2015/08/20/received PY - 2015/11/02/accepted PY - 2015/11/25/entrez PY - 2015/11/26/pubmed PY - 2016/6/21/medline SP - e0143254 EP - e0143254 JF - PloS one JO - PLoS One VL - 10 IS - 11 N2 - The global distribution of the soil-dwelling bacterium Burkholderia pseudomallei, causative agent of melioidosis, is poorly understood. We used established culturing methods developed for B. pseudomallei to isolate Burkholderia species from soil collected at 18 sampling sites in three states in the southern United States (Arizona (n = 4), Florida (n = 7), and Louisiana (n = 7)). Using multi-locus sequence typing (MLST) of seven genes, we identified 35 Burkholderia isolates from these soil samples. All species belonged to the B. cepacia complex (Bcc), including B. cenocepacia, B. cepacia, B. contaminans, B. diffusa, B. metallica, B. seminalis, B. vietnamiensis and two unnamed members of the Bcc. The MLST analysis provided a high level of resolution among and within these species. Despite previous clinical cases within the U.S. involving B. pseudomallei and its close phylogenetic relatives, we did not isolate any of these taxa. The Bcc contains a number of opportunistic pathogens that cause infections in cystic fibrosis patients. Interestingly, we found that B. vietnamiensis was present in soil from all three states, suggesting it may be a common component in southern U.S. soils. Most of the Burkholderia isolates collected in this study were from Florida (30/35; 86%), which may be due to the combination of relatively moist, sandy, and acidic soils found there compared to the other two states. We also investigated one MLST gene, recA, for its ability to identify species within Burkholderia. A 365bp fragment of recA recovered nearly the same species-level identification as MLST, thus demonstrating its cost effective utility when conducting environmental surveys for Burkholderia. Although we did not find B. pseudomallei, our findings document that other diverse Burkholderia species are present in soils in the southern United States. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/26600238/Diverse_Burkholderia_Species_Isolated_from_Soils_in_the_Southern_United_States_with_No_Evidence_of_B__pseudomallei_ L2 - https://dx.plos.org/10.1371/journal.pone.0143254 DB - PRIME DP - Unbound Medicine ER -