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Genome-wide identification of rubber tree (Hevea brasiliensis Muell. Arg.) aquaporin genes and their response to ethephon stimulation in the laticifer, a rubber-producing tissue.
BMC Genomics. 2015 Nov 25; 16:1001.BG

Abstract

BACKGROUND

Natural rubber, an important industrial raw material, is specifically synthesized in laticifers located inside the rubber tree (Hevea brasiliensis Muell. Arg.) trunk. Due to the absence of plasmodesmata, the laticifer water balance is mediated by aquaporins (AQPs). However, to date, the characterization of H. brasiliensis AQPs (HbAQPs) is still in its infancy.

RESULTS

In this study, 51 full-length AQP genes were identified from the rubber tree genome. The phylogenetic analysis assigned these AQPs to five subfamilies, including 15 plasma membrane intrinsic proteins (PIPs), 17 tonoplast intrinsic proteins (TIPs), 9 NOD26-like intrinsic proteins (NIPs), 4 small basic intrinsic proteins (SIPs) and 6 X intrinsic proteins (XIPs). Functional prediction based on the analysis of the aromatic/arginine (ar/R) selectivity filter, Froger's positions and specificity-determining positions (SDPs) showed a remarkable difference in substrate specificity among subfamilies. Homology analysis supported the expression of 44 HbAQP genes in at least one of the examined tissues. Furthermore, deep sequencing of the laticifer transcriptome in the form of latex revealed a key role of several PIP subfamily members in the laticifer water balance, and qRT-PCR analysis showed diverse expression patterns of laticifer-expressed HbAQP genes upon ethephon treatment, a widely-used practice for the stimulation of latex yield.

CONCLUSIONS

This study provides an important genetic resource of HbAQP genes, which will be useful to improve the water use efficiency and latex yield of Hevea.

Authors+Show Affiliations

Danzhou Investigation & Experiment Station of Tropical Crops, Ministry of Agriculture, Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou, 571737, P. R. China. zouzhi2008@126.com.Danzhou Investigation & Experiment Station of Tropical Crops, Ministry of Agriculture, Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou, 571737, P. R. China. 378201617@qq.com.Danzhou Investigation & Experiment Station of Tropical Crops, Ministry of Agriculture, Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou, 571737, P. R. China. an-f@163.com.Danzhou Investigation & Experiment Station of Tropical Crops, Ministry of Agriculture, Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou, 571737, P. R. China. xie23300459@163.com.Danzhou Investigation & Experiment Station of Tropical Crops, Ministry of Agriculture, Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou, 571737, P. R. China. kunjiwang@163.com.Danzhou Investigation & Experiment Station of Tropical Crops, Ministry of Agriculture, Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou, 571737, P. R. China. 23306788@163.com.Danzhou Investigation & Experiment Station of Tropical Crops, Ministry of Agriculture, Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou, 571737, P. R. China. ylfri@126.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26606923

Citation

Zou, Zhi, et al. "Genome-wide Identification of Rubber Tree (Hevea Brasiliensis Muell. Arg.) Aquaporin Genes and Their Response to Ethephon Stimulation in the Laticifer, a Rubber-producing Tissue." BMC Genomics, vol. 16, 2015, p. 1001.
Zou Z, Gong J, An F, et al. Genome-wide identification of rubber tree (Hevea brasiliensis Muell. Arg.) aquaporin genes and their response to ethephon stimulation in the laticifer, a rubber-producing tissue. BMC Genomics. 2015;16:1001.
Zou, Z., Gong, J., An, F., Xie, G., Wang, J., Mo, Y., & Yang, L. (2015). Genome-wide identification of rubber tree (Hevea brasiliensis Muell. Arg.) aquaporin genes and their response to ethephon stimulation in the laticifer, a rubber-producing tissue. BMC Genomics, 16, 1001. https://doi.org/10.1186/s12864-015-2152-6
Zou Z, et al. Genome-wide Identification of Rubber Tree (Hevea Brasiliensis Muell. Arg.) Aquaporin Genes and Their Response to Ethephon Stimulation in the Laticifer, a Rubber-producing Tissue. BMC Genomics. 2015 Nov 25;16:1001. PubMed PMID: 26606923.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genome-wide identification of rubber tree (Hevea brasiliensis Muell. Arg.) aquaporin genes and their response to ethephon stimulation in the laticifer, a rubber-producing tissue. AU - Zou,Zhi, AU - Gong,Jun, AU - An,Feng, AU - Xie,Guishui, AU - Wang,Jikun, AU - Mo,Yeyong, AU - Yang,Lifu, Y1 - 2015/11/25/ PY - 2015/09/19/received PY - 2015/10/27/accepted PY - 2015/11/27/entrez PY - 2015/11/27/pubmed PY - 2016/9/10/medline SP - 1001 EP - 1001 JF - BMC genomics JO - BMC Genomics VL - 16 N2 - BACKGROUND: Natural rubber, an important industrial raw material, is specifically synthesized in laticifers located inside the rubber tree (Hevea brasiliensis Muell. Arg.) trunk. Due to the absence of plasmodesmata, the laticifer water balance is mediated by aquaporins (AQPs). However, to date, the characterization of H. brasiliensis AQPs (HbAQPs) is still in its infancy. RESULTS: In this study, 51 full-length AQP genes were identified from the rubber tree genome. The phylogenetic analysis assigned these AQPs to five subfamilies, including 15 plasma membrane intrinsic proteins (PIPs), 17 tonoplast intrinsic proteins (TIPs), 9 NOD26-like intrinsic proteins (NIPs), 4 small basic intrinsic proteins (SIPs) and 6 X intrinsic proteins (XIPs). Functional prediction based on the analysis of the aromatic/arginine (ar/R) selectivity filter, Froger's positions and specificity-determining positions (SDPs) showed a remarkable difference in substrate specificity among subfamilies. Homology analysis supported the expression of 44 HbAQP genes in at least one of the examined tissues. Furthermore, deep sequencing of the laticifer transcriptome in the form of latex revealed a key role of several PIP subfamily members in the laticifer water balance, and qRT-PCR analysis showed diverse expression patterns of laticifer-expressed HbAQP genes upon ethephon treatment, a widely-used practice for the stimulation of latex yield. CONCLUSIONS: This study provides an important genetic resource of HbAQP genes, which will be useful to improve the water use efficiency and latex yield of Hevea. SN - 1471-2164 UR - https://www.unboundmedicine.com/medline/citation/26606923/Genome_wide_identification_of_rubber_tree__Hevea_brasiliensis_Muell__Arg___aquaporin_genes_and_their_response_to_ethephon_stimulation_in_the_laticifer_a_rubber_producing_tissue_ L2 - https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-015-2152-6 DB - PRIME DP - Unbound Medicine ER -