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Parental exposure to the herbicide diuron results in oxidative DNA damage to germinal cells of the Pacific oyster Crassostrea gigas.

Abstract

Chemical pollution by pesticides has been identified as a possible contributing factor to the massive mortality outbreaks observed in Crassostrea gigas for several years. A previous study demonstrated the vertical transmission of DNA damage by subjecting oyster genitors to the herbicide diuron at environmental concentrations during gametogenesis. This trans-generational effect occurs through damage to genitor-exposed gametes, as measured by the comet-assay. The presence of DNA damage in gametes could be linked to the formation of DNA damage in other germ cells. In order to explore this question, the levels and cell distribution of the oxidized base lesion 8-oxodGuo were studied in the gonads of exposed genitors. High-performance liquid chromatography coupled with UV and electrochemical detection analysis showed an increase in 8-oxodGuo levels in both male and female gonads after exposure to diuron. Immunohistochemistry analysis showed the presence of 8-oxodGuo at all stages of male germ cells, from early to mature stages. Conversely, the oxidized base was only present in early germ cell stages in female gonads. These results indicate that male and female genitors underwent oxidative stress following exposure to diuron, resulting in DNA oxidation in both early germ cells and gametes, such as spermatozoa, which could explain the transmission of diuron-induced DNA damage to offspring. Furthermore, immunostaining of early germ cells seems indicates that damages caused by exposure to diuron on germ line not only affect the current sexual cycle but also could affect future gametogenesis.

Authors+Show Affiliations

Ifremer, Department of Biogeochemistry and Ecotoxicology, Laboratory of Ecotoxicology, Rue de l'Ile d'Yeu, BP 21105, 44311 Nantes Cedex 03, France; Ifremer, SG2M, Laboratory of Genetics and Pathology of Marine Molluscs, Avenue de Mus du Loup, 17390 La Tremblade, France. Electronic address: audrey.barranger@gmail.com.Unité Biologie des organismes et écosystèmes aquatiques (BOREA, UMR 7208), Université de Caen Basse-Normandie, Sorbonne Universités, Muséum national d'Histoire naturelle, Université Pierre et Marie Curie, CNRS, IRD, 57 Rue Cuvier, 75005 Paris, France.Ifremer, Department of Biogeochemistry and Ecotoxicology, Laboratory of Ecotoxicology, Rue de l'Ile d'Yeu, BP 21105, 44311 Nantes Cedex 03, France.Unité Biologie des organismes et écosystèmes aquatiques (BOREA, UMR 7208), Université de Caen Basse-Normandie, Sorbonne Universités, Muséum national d'Histoire naturelle, Université Pierre et Marie Curie, CNRS, IRD, 57 Rue Cuvier, 75005 Paris, France.Ifremer, SG2M, Laboratory of Genetics and Pathology of Marine Molluscs, Avenue de Mus du Loup, 17390 La Tremblade, France.Ifremer, Department of Biogeochemistry and Ecotoxicology, Laboratory of Ecotoxicology, Rue de l'Ile d'Yeu, BP 21105, 44311 Nantes Cedex 03, France.Ifremer, Department of Biogeochemistry and Ecotoxicology, Laboratory of Ecotoxicology, Rue de l'Ile d'Yeu, BP 21105, 44311 Nantes Cedex 03, France. Electronic address: farida.akcha@ifremer.fr.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26610786

Citation

Barranger, Audrey, et al. "Parental Exposure to the Herbicide Diuron Results in Oxidative DNA Damage to Germinal Cells of the Pacific Oyster Crassostrea Gigas." Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP, vol. 180, 2016, pp. 23-30.
Barranger A, Heude-Berthelin C, Rouxel J, et al. Parental exposure to the herbicide diuron results in oxidative DNA damage to germinal cells of the Pacific oyster Crassostrea gigas. Comp Biochem Physiol C Toxicol Pharmacol. 2016;180:23-30.
Barranger, A., Heude-Berthelin, C., Rouxel, J., Adeline, B., Benabdelmouna, A., Burgeot, T., & Akcha, F. (2016). Parental exposure to the herbicide diuron results in oxidative DNA damage to germinal cells of the Pacific oyster Crassostrea gigas. Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP, 180, 23-30. https://doi.org/10.1016/j.cbpc.2015.11.002
Barranger A, et al. Parental Exposure to the Herbicide Diuron Results in Oxidative DNA Damage to Germinal Cells of the Pacific Oyster Crassostrea Gigas. Comp Biochem Physiol C Toxicol Pharmacol. 2016;180:23-30. PubMed PMID: 26610786.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Parental exposure to the herbicide diuron results in oxidative DNA damage to germinal cells of the Pacific oyster Crassostrea gigas. AU - Barranger,Audrey, AU - Heude-Berthelin,Clothilde, AU - Rouxel,Julien, AU - Adeline,Béatrice, AU - Benabdelmouna,Abdellah, AU - Burgeot,Thierry, AU - Akcha,Farida, Y1 - 2015/11/21/ PY - 2015/08/24/received PY - 2015/11/16/revised PY - 2015/11/18/accepted PY - 2015/11/28/entrez PY - 2015/11/28/pubmed PY - 2016/10/8/medline KW - Crassostrea gigas KW - DNA damage KW - Germ cell KW - Oxidative stress KW - Pesticides SP - 23 EP - 30 JF - Comparative biochemistry and physiology. Toxicology & pharmacology : CBP JO - Comp Biochem Physiol C Toxicol Pharmacol VL - 180 N2 - Chemical pollution by pesticides has been identified as a possible contributing factor to the massive mortality outbreaks observed in Crassostrea gigas for several years. A previous study demonstrated the vertical transmission of DNA damage by subjecting oyster genitors to the herbicide diuron at environmental concentrations during gametogenesis. This trans-generational effect occurs through damage to genitor-exposed gametes, as measured by the comet-assay. The presence of DNA damage in gametes could be linked to the formation of DNA damage in other germ cells. In order to explore this question, the levels and cell distribution of the oxidized base lesion 8-oxodGuo were studied in the gonads of exposed genitors. High-performance liquid chromatography coupled with UV and electrochemical detection analysis showed an increase in 8-oxodGuo levels in both male and female gonads after exposure to diuron. Immunohistochemistry analysis showed the presence of 8-oxodGuo at all stages of male germ cells, from early to mature stages. Conversely, the oxidized base was only present in early germ cell stages in female gonads. These results indicate that male and female genitors underwent oxidative stress following exposure to diuron, resulting in DNA oxidation in both early germ cells and gametes, such as spermatozoa, which could explain the transmission of diuron-induced DNA damage to offspring. Furthermore, immunostaining of early germ cells seems indicates that damages caused by exposure to diuron on germ line not only affect the current sexual cycle but also could affect future gametogenesis. SN - 1532-0456 UR - https://www.unboundmedicine.com/medline/citation/26610786/Parental_exposure_to_the_herbicide_diuron_results_in_oxidative_DNA_damage_to_germinal_cells_of_the_Pacific_oyster_Crassostrea_gigas_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1532-0456(15)00169-6 DB - PRIME DP - Unbound Medicine ER -