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Study on chemical analysis, antioxidant and in vitro antifungal activities of essential oil from wild Vitex agnus-castus L. seeds growing in area of Argan Tree of Morocco against clinical strains of Candida responsible for nosocomial infections.
J Mycol Med. 2015 Dec; 25(4):e118-27.JM

Abstract

OBJECTIVE

To study the composition, the antioxidant activity and the in vitro antifungal action anti-Candida species of essential oils extracted from seeds of Vite xagnus-castus L.

MATERIALS AND METHOD

The essential oils were extracted using Clevenger-type apparatus and analyzed by gas chromatography/mass spectrometry (GC/MS). The antioxidant activity was analyzed using the DPPH free radical-scavenging method. Susceptibility tests for Candida albicans (12), C. dubliniensis (1), C. glabrata (3), C. krusei (3), C. parapsilosis (6), C. lusitaniae (1), C. famata (1) and C. tropicalis (3) were expressed as inhibition zone by the disc-diffusion method and as minimal inhibitory concentration (MIC) and minimal fungicidal concentration (MFC) by the broth macrodilution method, compared to amphotricin B and fluconazol as standard drugs.

RESULTS

Major components were: 1,8-cineole (19.61%), sabinene (14.57%), α-pinene (9.76%), β-farnesene (6.04%), β-caryophyllene oxide (5.83%) and β-caryophyllene (5.02%). A low antioxidant activity was found (IC50=1.072mg/ml), but it can be exploited. V. agnus-castus seeds essential oils disosed a 35-58mm zone of inhibition (mean: 49mm) against all 30 isolates tested. In broth macrodilution method, all the tested Candida species were susceptible to the essential oils and this activity was concentration-dependent. MIC values varied from 0.13 to 2.13mg/ml V. agnus-castus seeds essential oils.

CONCLUSION

Results of this study indicated that the oils of plant origin could be used as potential anti-Candida species causative agents of nosocomial infections. These oils exhibited a noticeable antifungal activity against the selected fungi. The natural antifungal substances are inexpensive and have fewer side effects, they may represent alternative therapies for candidiasis.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Asdadi A
Équipe Planta Sud, laboratoire de biotechnologies végétales, faculté des sciences d'Agadir, université Ibn Zohr, BP 28/S, Agadir, Morocco. Electronic address: asdadiali@gmail.com.
Hamdouch A
Équipe Planta Sud, laboratoire de biotechnologies végétales, faculté des sciences d'Agadir, université Ibn Zohr, BP 28/S, Agadir, Morocco.
Oukacha A
Laboratoire de biotechnologies végétales, lutte biologique et phytochimie, faculté des sciences d'Agadir, université Ibn Zohr, BP 28/S, Agadir, Morocco.
Moutaj R
Laboratoire de parasitologie et de mycologie, hôpital militaire Avicenne, CHU Med VI, 40000 Marrakech, Morocco.
Gharby S
Laboratoire de chimie des plantes et de synthèse organique et bioorganique, faculté des sciences, université Mohammed V-Agdal, BP 1014, Rabat, Morocco.
Harhar H
Laboratoire de chimie des plantes et de synthèse organique et bioorganique, faculté des sciences, université Mohammed V-Agdal, BP 1014, Rabat, Morocco.
El Hadek M
Laboratoire de génie des procédés, faculté des sciences d'Agadir, université Ibn Zohr, BP 28/S, Agadir, Morocco.
Chebli B
Laboratoire de mécanique de procédé de l'énergie et de l'environnement, école nationale des sciences appliquées, université Ibn Zohr, BP 1136, Agadir, Morocco.
Idrissi Hassani LM
Équipe Planta Sud, laboratoire de biotechnologies végétales, faculté des sciences d'Agadir, université Ibn Zohr, BP 28/S, Agadir, Morocco.

MeSH

Antifungal AgentsAntioxidantsCandidaCrops, AgriculturalCross InfectionGas Chromatography-Mass SpectrometryHumansMicrobial Sensitivity TestsMoroccoOils, VolatilePlant OilsSeedsTreesVitex

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26611404

Citation

Asdadi, A, et al. "Study On Chemical Analysis, Antioxidant and in Vitro Antifungal Activities of Essential Oil From Wild Vitex Agnus-castus L. Seeds Growing in Area of Argan Tree of Morocco Against Clinical Strains of Candida Responsible for Nosocomial Infections." Journal De Mycologie Medicale, vol. 25, no. 4, 2015, pp. e118-27.
Asdadi A, Hamdouch A, Oukacha A, et al. Study on chemical analysis, antioxidant and in vitro antifungal activities of essential oil from wild Vitex agnus-castus L. seeds growing in area of Argan Tree of Morocco against clinical strains of Candida responsible for nosocomial infections. J Mycol Med. 2015;25(4):e118-27.
Asdadi, A., Hamdouch, A., Oukacha, A., Moutaj, R., Gharby, S., Harhar, H., El Hadek, M., Chebli, B., & Idrissi Hassani, L. M. (2015). Study on chemical analysis, antioxidant and in vitro antifungal activities of essential oil from wild Vitex agnus-castus L. seeds growing in area of Argan Tree of Morocco against clinical strains of Candida responsible for nosocomial infections. Journal De Mycologie Medicale, 25(4), e118-27. https://doi.org/10.1016/j.mycmed.2015.10.005
Asdadi A, et al. Study On Chemical Analysis, Antioxidant and in Vitro Antifungal Activities of Essential Oil From Wild Vitex Agnus-castus L. Seeds Growing in Area of Argan Tree of Morocco Against Clinical Strains of Candida Responsible for Nosocomial Infections. J Mycol Med. 2015;25(4):e118-27. PubMed PMID: 26611404.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Study on chemical analysis, antioxidant and in vitro antifungal activities of essential oil from wild Vitex agnus-castus L. seeds growing in area of Argan Tree of Morocco against clinical strains of Candida responsible for nosocomial infections. AU - Asdadi,A, AU - Hamdouch,A, AU - Oukacha,A, AU - Moutaj,R, AU - Gharby,S, AU - Harhar,H, AU - El Hadek,M, AU - Chebli,B, AU - Idrissi Hassani,L M, Y1 - 2015/11/21/ PY - 2015/05/01/received PY - 2015/09/27/revised PY - 2015/10/04/accepted PY - 2015/11/28/entrez PY - 2015/11/28/pubmed PY - 2016/12/15/medline KW - Antifongique KW - Antifungal KW - Antioxidant activities KW - Candida sp. activité antioxydante KW - Candida species KW - Essential oil of seeds KW - GC/MS KW - Huile essentielle KW - Infections nosocomiales KW - Nosocomial infections KW - Vitex agnus-castus L. SP - e118 EP - 27 JF - Journal de mycologie medicale JO - J Mycol Med VL - 25 IS - 4 N2 - OBJECTIVE: To study the composition, the antioxidant activity and the in vitro antifungal action anti-Candida species of essential oils extracted from seeds of Vite xagnus-castus L. MATERIALS AND METHOD: The essential oils were extracted using Clevenger-type apparatus and analyzed by gas chromatography/mass spectrometry (GC/MS). The antioxidant activity was analyzed using the DPPH free radical-scavenging method. Susceptibility tests for Candida albicans (12), C. dubliniensis (1), C. glabrata (3), C. krusei (3), C. parapsilosis (6), C. lusitaniae (1), C. famata (1) and C. tropicalis (3) were expressed as inhibition zone by the disc-diffusion method and as minimal inhibitory concentration (MIC) and minimal fungicidal concentration (MFC) by the broth macrodilution method, compared to amphotricin B and fluconazol as standard drugs. RESULTS: Major components were: 1,8-cineole (19.61%), sabinene (14.57%), α-pinene (9.76%), β-farnesene (6.04%), β-caryophyllene oxide (5.83%) and β-caryophyllene (5.02%). A low antioxidant activity was found (IC50=1.072mg/ml), but it can be exploited. V. agnus-castus seeds essential oils disosed a 35-58mm zone of inhibition (mean: 49mm) against all 30 isolates tested. In broth macrodilution method, all the tested Candida species were susceptible to the essential oils and this activity was concentration-dependent. MIC values varied from 0.13 to 2.13mg/ml V. agnus-castus seeds essential oils. CONCLUSION: Results of this study indicated that the oils of plant origin could be used as potential anti-Candida species causative agents of nosocomial infections. These oils exhibited a noticeable antifungal activity against the selected fungi. The natural antifungal substances are inexpensive and have fewer side effects, they may represent alternative therapies for candidiasis. SN - 1773-0449 UR - https://www.unboundmedicine.com/medline/citation/26611404/Study_on_chemical_analysis_antioxidant_and_in_vitro_antifungal_activities_of_essential_oil_from_wild_Vitex_agnus_castus_L__seeds_growing_in_area_of_Argan_Tree_of_Morocco_against_clinical_strains_of_Candida_responsible_for_nosocomial_infections_ DB - PRIME DP - Unbound Medicine ER -