Tags

Type your tag names separated by a space and hit enter

Phytochemical constituents and biological activities of different extracts of Strobilanthes crispus (L.) Bremek leaves grown in different locations of Malaysia.
BMC Complement Altern Med. 2015 Nov 27; 15(1):422.BC

Abstract

BACKGROUND

Strobilanthes crispus is a well-known herb in Malaysia with various pharmaceutical properties. S. crispus is known to contain several biologically active chemical constituents which are responsible for its pharmaceutical quality.

METHODS

Strobilanthes crispus leaves grown in three different locations in Malaysia [Kelantan (North-east), Selangor (Central), and Penang (North)], were investigated for differences in the content of secondary metabolites [total phenolics content (TPC), total flavonoids content (TFC), and total saponins content (TSC)] as well as for their antioxidant and anticancer properties. Phenolic acids and flavonoids were identified using ultra-high performance liquid chromatography (UHPLC). Ferric reducing antioxidant potential (FRAP) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) assays were used to evaluate the antioxidant activities. The anticancer activity of extracts against HeLa cancer cell line was evaluated using the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay.

RESULTS

Samples from the three different locations when extracted with two solvents (aqueous and ethanol extracts) yielded significantly different results for TPC, TFC, and TSC as well as for antioxidant activity. Aqueous extract of S. crispus leaves collected from Kelantan exhibited the highest values: TPC [12.62 mg gallic acid equivalents (GAE)/g dry matter (DM)], TFC (7.44 mg quercetin equivalents (QE)/g DM), TSC (44.7 mg diosgenin equivalents (DE)/g DM), DPPH (73.8 %), and FRAP (267.5 μM of Fe (II)/g) activity with a half-maximal inhibitory concentration (IC50) of 44.1 μg/mL compared to the extracts of leaves collected from the other two locations. The most important secondary metabolites identified in this study, based on concentration, were phenolics classified as followed: caffeic acid>ferulic acid>gallic acid>chlorogenic acid>trans-cinnamic acid; flavonoids: quercetin>rutin>catechin>apigenin>naringenin>kaempferol. Extracts of leaves collected from Kelantan exhibited better anticancer activity against HeLa cancer cell line with an IC50 of 182.5 μg/mL compared to the extracts of leaves from Selangor (IC50 = 266.4 μg/mL) and Penang (IC50 = 331.5 μg/mL) and to tamoxifen (IC50 = 63.4 μg/mL). S. crispus leaves with the highest content of secondary metabolites exhibited the most potent antioxidant and anticancer activity.

CONCLUSIONS

Therefore, based on the potent antioxidant and anticancer activity of leaves extracts, it appears that S. crispus grown in the North-east of Malaysia (Kelantan) is a potential source of anticarcinogenic therapeutic compounds.

Authors+Show Affiliations

Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia. alighasemzadeh@upm.edu.my.Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia. hawazej@upm.edu.my.Department of Nutrition & Dietetics, Faculty of Medicine & Health Sciences, Universiti Putra Malaysia, 43400, UPM, Serdang, Selangor, Malaysia. asmah@upm.edu.my.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26613959

Citation

Ghasemzadeh, Ali, et al. "Phytochemical Constituents and Biological Activities of Different Extracts of Strobilanthes Crispus (L.) Bremek Leaves Grown in Different Locations of Malaysia." BMC Complementary and Alternative Medicine, vol. 15, no. 1, 2015, p. 422.
Ghasemzadeh A, Jaafar HZ, Rahmat A. Phytochemical constituents and biological activities of different extracts of Strobilanthes crispus (L.) Bremek leaves grown in different locations of Malaysia. BMC Complement Altern Med. 2015;15(1):422.
Ghasemzadeh, A., Jaafar, H. Z., & Rahmat, A. (2015). Phytochemical constituents and biological activities of different extracts of Strobilanthes crispus (L.) Bremek leaves grown in different locations of Malaysia. BMC Complementary and Alternative Medicine, 15(1), 422. https://doi.org/10.1186/s12906-015-0873-3
Ghasemzadeh A, Jaafar HZ, Rahmat A. Phytochemical Constituents and Biological Activities of Different Extracts of Strobilanthes Crispus (L.) Bremek Leaves Grown in Different Locations of Malaysia. BMC Complement Altern Med. 2015 Nov 27;15(1):422. PubMed PMID: 26613959.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Phytochemical constituents and biological activities of different extracts of Strobilanthes crispus (L.) Bremek leaves grown in different locations of Malaysia. AU - Ghasemzadeh,Ali, AU - Jaafar,Hawa Z E, AU - Rahmat,Asmah, Y1 - 2015/11/27/ PY - 2015/06/02/received PY - 2015/09/23/accepted PY - 2015/11/29/entrez PY - 2015/11/29/pubmed PY - 2016/4/9/medline SP - 422 EP - 422 JF - BMC complementary and alternative medicine JO - BMC Complement Altern Med VL - 15 IS - 1 N2 - BACKGROUND: Strobilanthes crispus is a well-known herb in Malaysia with various pharmaceutical properties. S. crispus is known to contain several biologically active chemical constituents which are responsible for its pharmaceutical quality. METHODS: Strobilanthes crispus leaves grown in three different locations in Malaysia [Kelantan (North-east), Selangor (Central), and Penang (North)], were investigated for differences in the content of secondary metabolites [total phenolics content (TPC), total flavonoids content (TFC), and total saponins content (TSC)] as well as for their antioxidant and anticancer properties. Phenolic acids and flavonoids were identified using ultra-high performance liquid chromatography (UHPLC). Ferric reducing antioxidant potential (FRAP) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) assays were used to evaluate the antioxidant activities. The anticancer activity of extracts against HeLa cancer cell line was evaluated using the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. RESULTS: Samples from the three different locations when extracted with two solvents (aqueous and ethanol extracts) yielded significantly different results for TPC, TFC, and TSC as well as for antioxidant activity. Aqueous extract of S. crispus leaves collected from Kelantan exhibited the highest values: TPC [12.62 mg gallic acid equivalents (GAE)/g dry matter (DM)], TFC (7.44 mg quercetin equivalents (QE)/g DM), TSC (44.7 mg diosgenin equivalents (DE)/g DM), DPPH (73.8 %), and FRAP (267.5 μM of Fe (II)/g) activity with a half-maximal inhibitory concentration (IC50) of 44.1 μg/mL compared to the extracts of leaves collected from the other two locations. The most important secondary metabolites identified in this study, based on concentration, were phenolics classified as followed: caffeic acid>ferulic acid>gallic acid>chlorogenic acid>trans-cinnamic acid; flavonoids: quercetin>rutin>catechin>apigenin>naringenin>kaempferol. Extracts of leaves collected from Kelantan exhibited better anticancer activity against HeLa cancer cell line with an IC50 of 182.5 μg/mL compared to the extracts of leaves from Selangor (IC50 = 266.4 μg/mL) and Penang (IC50 = 331.5 μg/mL) and to tamoxifen (IC50 = 63.4 μg/mL). S. crispus leaves with the highest content of secondary metabolites exhibited the most potent antioxidant and anticancer activity. CONCLUSIONS: Therefore, based on the potent antioxidant and anticancer activity of leaves extracts, it appears that S. crispus grown in the North-east of Malaysia (Kelantan) is a potential source of anticarcinogenic therapeutic compounds. SN - 1472-6882 UR - https://www.unboundmedicine.com/medline/citation/26613959/Phytochemical_constituents_and_biological_activities_of_different_extracts_of_Strobilanthes_crispus__L___Bremek_leaves_grown_in_different_locations_of_Malaysia_ L2 - https://bmccomplementalternmed.biomedcentral.com/articles/10.1186/s12906-015-0873-3 DB - PRIME DP - Unbound Medicine ER -