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Systemic expression of inflammatory mediators in patients with chronic rhinosinusitis and nasal polyps with and without Aspirin Exacerbated Respiratory Disease.
Cytokine. 2016 Jan; 77:157-67.C

Abstract

BACKGROUND

Systemic reactions are related to the pathogenesis of Aspirin Exacerbated Respiratory Disease (AERD). With this work we wanted to study the changes in the systemic levels of inflammatory mediators in both baseline and after oral aspirin challenge in patients with and without AERD.

METHODS

Patients with nasal polyposis and asthma with AERD (n=20) and without (n=18) were orally challenged with aspirin in a single-blind placebo controlled study. Serum samples and urine were collected before and 6h after placebo and aspirin oral challenges. Serum levels of inflammatory mediators were assayed by using the Luminex technology and ELISA. The concentrations of 9-alpha, 11-beta prostaglandin F2, and leukotriene E4 (uLTE4) were measured in urine samples by ELISA. The expression of T-cell surface markers was analyzed in peripheral blood mononuclear cells isolated before and after the challenges.

RESULTS

AERD patients showed significantly higher baseline levels of s-IL-5R-alpha, uLTE4 and percentage of CD4(+)CD25(+)CD127(pos) and CD4(+)CD45RA(-)CD45RO(+) but decreased levels of TGF-β1 and number of CD4(+)CD25(+)CD127(neg) cells. Aspirin challenge induced the release of uLTE4, IL-6 and increased the number of CD4(+)CD45RA(-)CD45RO(+) memory T-cells only in AERD patients but failed to reduce the levels of sCD40L as observed in non-AERD subjects. Further, IL-8 and sIL-5R-alpha levels directly correlated with the PD20ASA and the effects of aspirin on IL-6 and number of memory T-cells was more pronounced in subjects showing more strong reaction (bronchial and nasal).

CONCLUSIONS

AERD patients have a differential baseline inflammatory pattern that supports the role inflammation as underlying mechanism of the disease. Systemic response to oral aspirin challenge was related to an increase in serum IL-6 and the number of circulating memory T-cells in AERD patients.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Upper Airways Research Laboratory, Dept. of Otorhinolaryngology, Ghent University Hospital, Belgium; Dept. of Otorhinolaryngology, Head and Neck Surgery, Federal University of São Paulo, São Paulo, Brazil.

Świerczyńska-Krępa M

Out-patient Allergy Clinic, Medex, Bielsko-Biała, Poland.

Niżankowska-Mogilnicka E

Dept. of Medicine, Jagiellonian University School of Medicine, Cracow, Poland.

Upper Airways Research Laboratory, Dept. of Otorhinolaryngology, Ghent University Hospital, Belgium.

Upper Airways Research Laboratory, Dept. of Otorhinolaryngology, Ghent University Hospital, Belgium.

Dept. of Medicine, Jagiellonian University School of Medicine, Cracow, Poland.

Upper Airways Research Laboratory, Dept. of Otorhinolaryngology, Ghent University Hospital, Belgium.

Van Crombruggen K

Upper Airways Research Laboratory, Dept. of Otorhinolaryngology, Ghent University Hospital, Belgium.

Upper Airways Research Laboratory, Dept. of Otorhinolaryngology, Ghent University Hospital, Belgium.

Upper Airways Research Laboratory, Dept. of Otorhinolaryngology, Ghent University Hospital, Belgium. Electronic address: Claudina.Pereznovo@UGent.be.

MeSH

AdultAnti-Inflammatory Agents, Non-SteroidalAspirinAsthma, Aspirin-InducedChronic DiseaseCytokinesFemaleHumansImmunoenzyme TechniquesInflammation MediatorsLeukotriene E4MaleMiddle AgedNasal PolypsProstaglandin D2RhinitisSingle-Blind MethodSinusitisT-Lymphocyte Subsets

Pub Type(s)

Controlled Clinical Trial

Journal Article

Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26615369

Citation

Pezato, Rogerio, et al. "Systemic Expression of Inflammatory Mediators in Patients With Chronic Rhinosinusitis and Nasal Polyps With and Without Aspirin Exacerbated Respiratory Disease." Cytokine, vol. 77, 2016, pp. 157-67.

Pezato R, Świerczyńska-Krępa M, Niżankowska-Mogilnicka E, et al. Systemic expression of inflammatory mediators in patients with chronic rhinosinusitis and nasal polyps with and without Aspirin Exacerbated Respiratory Disease. Cytokine. 2016;77:157-67.

Pezato, R., Świerczyńska-Krępa, M., Niżankowska-Mogilnicka, E., Holtappels, G., De Ruyck, N., Sanak, M., Derycke, L., Van Crombruggen, K., Bachert, C., & Pérez-Novo, C. A. (2016). Systemic expression of inflammatory mediators in patients with chronic rhinosinusitis and nasal polyps with and without Aspirin Exacerbated Respiratory Disease. Cytokine, 77, 157-67. https://doi.org/10.1016/j.cyto.2015.10.011

Pezato R, et al. Systemic Expression of Inflammatory Mediators in Patients With Chronic Rhinosinusitis and Nasal Polyps With and Without Aspirin Exacerbated Respiratory Disease. Cytokine. 2016;77:157-67. PubMed PMID: 26615369.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Systemic expression of inflammatory mediators in patients with chronic rhinosinusitis and nasal polyps with and without Aspirin Exacerbated Respiratory Disease. AU - Pezato,Rogerio, AU - Świerczyńska-Krępa,Monika, AU - Niżankowska-Mogilnicka,Ewa, AU - Holtappels,Gabriele, AU - De Ruyck,Natalie, AU - Sanak,Marek, AU - Derycke,Lara, AU - Van Crombruggen,Koen, AU - Bachert,Claus, AU - Pérez-Novo,Claudina A, Y1 - 2015/11/23/ PY - 2015/07/15/received PY - 2015/10/22/revised PY - 2015/10/23/accepted PY - 2015/11/30/entrez PY - 2015/11/30/pubmed PY - 2016/9/15/medline KW - Aspirin Exacerbated Respiratory Disease KW - IL-5 receptor alpha KW - Interleukin 6 KW - Interleukin 8 KW - Memory T-cells KW - Oral aspirin challenge KW - Systemic reaction after aspirin challenge KW - TGF-β(1) SP - 157 EP - 67 JF - Cytokine JO - Cytokine VL - 77 N2 - BACKGROUND: Systemic reactions are related to the pathogenesis of Aspirin Exacerbated Respiratory Disease (AERD). With this work we wanted to study the changes in the systemic levels of inflammatory mediators in both baseline and after oral aspirin challenge in patients with and without AERD. METHODS: Patients with nasal polyposis and asthma with AERD (n=20) and without (n=18) were orally challenged with aspirin in a single-blind placebo controlled study. Serum samples and urine were collected before and 6h after placebo and aspirin oral challenges. Serum levels of inflammatory mediators were assayed by using the Luminex technology and ELISA. The concentrations of 9-alpha, 11-beta prostaglandin F2, and leukotriene E4 (uLTE4) were measured in urine samples by ELISA. The expression of T-cell surface markers was analyzed in peripheral blood mononuclear cells isolated before and after the challenges. RESULTS: AERD patients showed significantly higher baseline levels of s-IL-5R-alpha, uLTE4 and percentage of CD4(+)CD25(+)CD127(pos) and CD4(+)CD45RA(-)CD45RO(+) but decreased levels of TGF-β1 and number of CD4(+)CD25(+)CD127(neg) cells. Aspirin challenge induced the release of uLTE4, IL-6 and increased the number of CD4(+)CD45RA(-)CD45RO(+) memory T-cells only in AERD patients but failed to reduce the levels of sCD40L as observed in non-AERD subjects. Further, IL-8 and sIL-5R-alpha levels directly correlated with the PD20ASA and the effects of aspirin on IL-6 and number of memory T-cells was more pronounced in subjects showing more strong reaction (bronchial and nasal). CONCLUSIONS: AERD patients have a differential baseline inflammatory pattern that supports the role inflammation as underlying mechanism of the disease. Systemic response to oral aspirin challenge was related to an increase in serum IL-6 and the number of circulating memory T-cells in AERD patients. SN - 1096-0023 UR - https://www.unboundmedicine.com/medline/citation/26615369/Systemic_expression_of_inflammatory_mediators_in_patients_with_chronic_rhinosinusitis_and_nasal_polyps_with_and_without_Aspirin_Exacerbated_Respiratory_Disease_ DB - PRIME DP - Unbound Medicine ER -