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Reverse micellar extraction of bromelain from pineapple peel--Effect of surfactant structure.
Food Chem. 2016 Apr 15; 197(Pt A):450-6.FC

Abstract

Pineapple peel is generally disposed or used as compost. This study was focused on extracting bromelain from pineapple peel by using reverse micelles. It was found that gemini surfactant C12-8-C12·2Br (octamethylene-α,ω-bis(dimethyldodecylammonium bromide)) showed distinctive advantage over its monomeric counterpart DTAB (dodecyl trimethyl ammonium bromide); under optimized condition, the bromelain extracted with C12-8-C12·2Br reverse micelle had an activity recovery of 163% and a purification fold of 3.3, while when using DTAB reverse micelle, the activity recovery was 95% and the purification fold was 1.7. Therefore, the spacer of gemini surfactant should play a positive role in bromelain extraction and may suggest the potential of gemini surfactant in protein separation since it has been so far rarely used in relative experiments or technologies.

Authors+Show Affiliations

School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou, Jiangsu 225002, PR China.School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou, Jiangsu 225002, PR China.School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou, Jiangsu 225002, PR China.School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou, Jiangsu 225002, PR China. Electronic address: guoxia@yzu.edu.cn.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26616974

Citation

Wan, Jing, et al. "Reverse Micellar Extraction of Bromelain From Pineapple peel--Effect of Surfactant Structure." Food Chemistry, vol. 197, no. Pt A, 2016, pp. 450-6.
Wan J, Guo J, Miao Z, et al. Reverse micellar extraction of bromelain from pineapple peel--Effect of surfactant structure. Food Chem. 2016;197(Pt A):450-6.
Wan, J., Guo, J., Miao, Z., & Guo, X. (2016). Reverse micellar extraction of bromelain from pineapple peel--Effect of surfactant structure. Food Chemistry, 197(Pt A), 450-6. https://doi.org/10.1016/j.foodchem.2015.10.145
Wan J, et al. Reverse Micellar Extraction of Bromelain From Pineapple peel--Effect of Surfactant Structure. Food Chem. 2016 Apr 15;197(Pt A):450-6. PubMed PMID: 26616974.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Reverse micellar extraction of bromelain from pineapple peel--Effect of surfactant structure. AU - Wan,Jing, AU - Guo,Jingjing, AU - Miao,Zhitong, AU - Guo,Xia, Y1 - 2015/11/02/ PY - 2015/03/26/received PY - 2015/10/27/revised PY - 2015/10/31/accepted PY - 2015/12/1/entrez PY - 2015/12/1/pubmed PY - 2016/8/4/medline KW - Bromelain KW - Pineapple peel KW - Protein extraction KW - Reverse micelle KW - Surfactant SP - 450 EP - 6 JF - Food chemistry JO - Food Chem VL - 197 IS - Pt A N2 - Pineapple peel is generally disposed or used as compost. This study was focused on extracting bromelain from pineapple peel by using reverse micelles. It was found that gemini surfactant C12-8-C12·2Br (octamethylene-α,ω-bis(dimethyldodecylammonium bromide)) showed distinctive advantage over its monomeric counterpart DTAB (dodecyl trimethyl ammonium bromide); under optimized condition, the bromelain extracted with C12-8-C12·2Br reverse micelle had an activity recovery of 163% and a purification fold of 3.3, while when using DTAB reverse micelle, the activity recovery was 95% and the purification fold was 1.7. Therefore, the spacer of gemini surfactant should play a positive role in bromelain extraction and may suggest the potential of gemini surfactant in protein separation since it has been so far rarely used in relative experiments or technologies. SN - 1873-7072 UR - https://www.unboundmedicine.com/medline/citation/26616974/Reverse_micellar_extraction_of_bromelain_from_pineapple_peel__Effect_of_surfactant_structure_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0308-8146(15)30144-8 DB - PRIME DP - Unbound Medicine ER -