Tags

Type your tag names separated by a space and hit enter

Flk-1⁺Sca-1⁻ mesenchymal stem cells: functional characteristics in vitro and regenerative capacity in vivo.
Int J Clin Exp Pathol. 2015; 8(9):9875-88.IJ

Abstract

OBJECTIVES

Mesenchymal stem cells (MSCs) represent a powerful tool in regenerative medicine because of their differentiation and migration capacities. We aimed to investigate the possibility of Flk-1(+)Sca-1(-) mesenchymal stem cells (Flk-1(+)Sca-1(-) MSCs) transplantation to repair erectile function in patients suffering from diabetes mellitus (DM)-associated erectile dysfunction (ED).

METHODS

In this study, we isolated Flk-1(+)Sca-1(-) MSCs from bone marrow (bMSCs). Then, newborn male rats were intraperitoneally injected with 5-ethynyl-2-deoxyuridine for the purpose of tracking endogenous Flk-1(+)Sca-1(-) MSCs. Eight weeks later, 8 of these rats were randomly chosen to serve as normal control (N group). The remaining rats were injected intraperitoneally with 60 mg/kg of streptozotocin (STZ) to induce DM. Eight of these rats were randomly chosen to serve as DM control (DM group) while another 8 rats were subject to Flk-1(+)Sca-1(-) MSCs treatment (DM+MSC group). All rats were evaluated for erectile function by intracavernous pressure (ICP) measurement. Afterward, their penile tissues were examined by histology.

RESULTS

Flk-1(+)Sca-1(-) MSCs could differentiate into skeletal muscle cells and endothelial cells in vivo and in vitro. Engrafted Flk-1(+)Sca-1(-) MSCs were shown to home to injured muscle, participate in myofibers repair and could partially reconstitute the sarcolemmal expression of myocardin and ameliorate the level of related specific pathological markers.

CONCLUSION

Flk-1(+)Sca-1(-) MSCs could be used in the treatment erectile function in diabetes mellitus associated erectile dysfunction by promoting regeneration of nNOS-positive nerves, endothelium, and smooth muscle in the penis.

Authors+Show Affiliations

Hospital of Integrated Traditional Chinese Medicine & Western Medicine, Southern Medical University Guangzhou 510315, China.Hospital of Integrated Traditional Chinese Medicine & Western Medicine, Southern Medical University Guangzhou 510315, China.Hospital of Integrated Traditional Chinese Medicine & Western Medicine, Southern Medical University Guangzhou 510315, China.Hospital of Integrated Traditional Chinese Medicine & Western Medicine, Southern Medical University Guangzhou 510315, China.Department of Urology, Medical Center for Overseas Patients, Nanfang Hospital, Southern Medical University Guangzhou 510515, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26617697

Citation

Li, Yugang, et al. "Flk-1⁺Sca-1⁻ Mesenchymal Stem Cells: Functional Characteristics in Vitro and Regenerative Capacity in Vivo." International Journal of Clinical and Experimental Pathology, vol. 8, no. 9, 2015, pp. 9875-88.
Li Y, Pan E, Wang Y, et al. Flk-1⁺Sca-1⁻ mesenchymal stem cells: functional characteristics in vitro and regenerative capacity in vivo. Int J Clin Exp Pathol. 2015;8(9):9875-88.
Li, Y., Pan, E., Wang, Y., Zhu, X., & Wei, A. (2015). Flk-1⁺Sca-1⁻ mesenchymal stem cells: functional characteristics in vitro and regenerative capacity in vivo. International Journal of Clinical and Experimental Pathology, 8(9), 9875-88.
Li Y, et al. Flk-1⁺Sca-1⁻ Mesenchymal Stem Cells: Functional Characteristics in Vitro and Regenerative Capacity in Vivo. Int J Clin Exp Pathol. 2015;8(9):9875-88. PubMed PMID: 26617697.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Flk-1⁺Sca-1⁻ mesenchymal stem cells: functional characteristics in vitro and regenerative capacity in vivo. AU - Li,Yugang, AU - Pan,Enshan, AU - Wang,Yu, AU - Zhu,Xiaoguang, AU - Wei,Anyang, Y1 - 2015/09/01/ PY - 2015/06/01/received PY - 2015/07/21/accepted PY - 2015/12/1/entrez PY - 2015/12/1/pubmed PY - 2016/9/30/medline KW - Flk-1+Sca-1- mesenchymal stem cells (Flk-1+Sca-1- MSCs) KW - diabetes mellitus (DM) KW - erectile dysfunction (ED) KW - myocardin KW - nNOS SP - 9875 EP - 88 JF - International journal of clinical and experimental pathology JO - Int J Clin Exp Pathol VL - 8 IS - 9 N2 - OBJECTIVES: Mesenchymal stem cells (MSCs) represent a powerful tool in regenerative medicine because of their differentiation and migration capacities. We aimed to investigate the possibility of Flk-1(+)Sca-1(-) mesenchymal stem cells (Flk-1(+)Sca-1(-) MSCs) transplantation to repair erectile function in patients suffering from diabetes mellitus (DM)-associated erectile dysfunction (ED). METHODS: In this study, we isolated Flk-1(+)Sca-1(-) MSCs from bone marrow (bMSCs). Then, newborn male rats were intraperitoneally injected with 5-ethynyl-2-deoxyuridine for the purpose of tracking endogenous Flk-1(+)Sca-1(-) MSCs. Eight weeks later, 8 of these rats were randomly chosen to serve as normal control (N group). The remaining rats were injected intraperitoneally with 60 mg/kg of streptozotocin (STZ) to induce DM. Eight of these rats were randomly chosen to serve as DM control (DM group) while another 8 rats were subject to Flk-1(+)Sca-1(-) MSCs treatment (DM+MSC group). All rats were evaluated for erectile function by intracavernous pressure (ICP) measurement. Afterward, their penile tissues were examined by histology. RESULTS: Flk-1(+)Sca-1(-) MSCs could differentiate into skeletal muscle cells and endothelial cells in vivo and in vitro. Engrafted Flk-1(+)Sca-1(-) MSCs were shown to home to injured muscle, participate in myofibers repair and could partially reconstitute the sarcolemmal expression of myocardin and ameliorate the level of related specific pathological markers. CONCLUSION: Flk-1(+)Sca-1(-) MSCs could be used in the treatment erectile function in diabetes mellitus associated erectile dysfunction by promoting regeneration of nNOS-positive nerves, endothelium, and smooth muscle in the penis. SN - 1936-2625 UR - https://www.unboundmedicine.com/medline/citation/26617697/Flk_1⁺Sca_1⁻_mesenchymal_stem_cells:_functional_characteristics_in_vitro_and_regenerative_capacity_in_vivo_ L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/26617697/ DB - PRIME DP - Unbound Medicine ER -