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Blockage of thymic stromal lymphopoietin signaling improves acute lung injury in mice by regulating pulmonary dendritic cells.
Int J Clin Exp Pathol 2015; 8(9):10698-706IJ

Abstract

OBJECTIVES

To investigate the effects of blockage of thymic stromal lymphopoietin (TSLP) signaling by TSLP receptor (TSLPR)-immunoglobulin (Ig) on acute lung injury (ALI) induced by lipopolysaccharide (LPS).

METHODS

C57BL/6 mice received TSLPR-Ig or controlled-Ig before being induced ALI. Lung wet/dry (W/D) weight ratio was recorded. Neutrophil number and albumin concentration of bronchoalveolar lavages fluids (BALF) were determined. Besides, bone marrow dendritic cells (BMDCs) were separated and cultured with medium, TSLP, TSLP plus TSLPR-Ig or TSLP plus controlled-Ig. Protein expression levels of TSLP in lung tissues, phosphorylation extracellular regulated protein kinases (pERK) 1/2, p38, and signal transducers and activators of transcription (STAT) 3 in BMDCs were analyzed using Western blotting. Expression of CD40, CD80 and CD86 on pulmonary DCs and BMDCs was determined using flow cytometry (FCM).

RESULTS

The W/D ratio, neutrophil number and albumin concentration were significantly decreased in the TSLPR-Ig group compared with the controlled-Ig and model group. Moreover, there was a noticeable decrease in CD40, CD80 or CD86 expression by TSLPR-Ig on both pulmonary DCs and BMDCs. The protein levels of TSLP, pERK1 and STAT3 were significantly decreased by TSLPR-Ig. However, no significant differences were found in p38 and pERK2.

CONCLUSION

These results suggest that TSLP may be involved in ALI, and blockage of TSLP signaling using TSLPR-Ig improves ALI at least in part by regulation of DCs functions. The underling downstream signaling mediated by TSLP might be associated with activating the ERK1 and STAT3 signaling pathway.

Authors+Show Affiliations

Department of Pediatric Intensive Care Unit, The Second Affiliated Hospital & Yuying Children's Hospital, Wenzhou Medical University Wenzhou 325000, Zhejiang, China.Department of Pediatric Intensive Care Unit, The Second Affiliated Hospital & Yuying Children's Hospital, Wenzhou Medical University Wenzhou 325000, Zhejiang, China.Department of Pediatric Intensive Care Unit, The Second Affiliated Hospital & Yuying Children's Hospital, Wenzhou Medical University Wenzhou 325000, Zhejiang, China.Department of Pediatric Intensive Care Unit, The Second Affiliated Hospital & Yuying Children's Hospital, Wenzhou Medical University Wenzhou 325000, Zhejiang, China.Department of Pediatric Intensive Care Unit, The Second Affiliated Hospital & Yuying Children's Hospital, Wenzhou Medical University Wenzhou 325000, Zhejiang, China.Department of Pediatric Intensive Care Unit, The Second Affiliated Hospital & Yuying Children's Hospital, Wenzhou Medical University Wenzhou 325000, Zhejiang, China.Department of Pediatric Intensive Care Unit, The Second Affiliated Hospital & Yuying Children's Hospital, Wenzhou Medical University Wenzhou 325000, Zhejiang, China.Department of Pediatric Intensive Care Unit, The Second Affiliated Hospital & Yuying Children's Hospital, Wenzhou Medical University Wenzhou 325000, Zhejiang, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26617780

Citation

Pan, Guoquan, et al. "Blockage of Thymic Stromal Lymphopoietin Signaling Improves Acute Lung Injury in Mice By Regulating Pulmonary Dendritic Cells." International Journal of Clinical and Experimental Pathology, vol. 8, no. 9, 2015, pp. 10698-706.
Pan G, Liang Y, Lu L, et al. Blockage of thymic stromal lymphopoietin signaling improves acute lung injury in mice by regulating pulmonary dendritic cells. Int J Clin Exp Pathol. 2015;8(9):10698-706.
Pan, G., Liang, Y., Lu, L., Chen, X., Wang, M., Wang, L., ... Zhang, W. (2015). Blockage of thymic stromal lymphopoietin signaling improves acute lung injury in mice by regulating pulmonary dendritic cells. International Journal of Clinical and Experimental Pathology, 8(9), pp. 10698-706.
Pan G, et al. Blockage of Thymic Stromal Lymphopoietin Signaling Improves Acute Lung Injury in Mice By Regulating Pulmonary Dendritic Cells. Int J Clin Exp Pathol. 2015;8(9):10698-706. PubMed PMID: 26617780.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Blockage of thymic stromal lymphopoietin signaling improves acute lung injury in mice by regulating pulmonary dendritic cells. AU - Pan,Guoquan, AU - Liang,Yafeng, AU - Lu,Lu, AU - Chen,Xu, AU - Wang,Min, AU - Wang,Linxia, AU - Yan,Chunxue, AU - Zhang,Weixi, Y1 - 2015/09/01/ PY - 2015/07/08/received PY - 2015/08/21/accepted PY - 2015/12/1/entrez PY - 2015/12/1/pubmed PY - 2016/9/27/medline KW - Thymic stromal lymphopoietin KW - acute lung injury KW - dendritic cells KW - lipopolysaccharide KW - thymic stromal lymphopoietin receptor SP - 10698 EP - 706 JF - International journal of clinical and experimental pathology JO - Int J Clin Exp Pathol VL - 8 IS - 9 N2 - OBJECTIVES: To investigate the effects of blockage of thymic stromal lymphopoietin (TSLP) signaling by TSLP receptor (TSLPR)-immunoglobulin (Ig) on acute lung injury (ALI) induced by lipopolysaccharide (LPS). METHODS: C57BL/6 mice received TSLPR-Ig or controlled-Ig before being induced ALI. Lung wet/dry (W/D) weight ratio was recorded. Neutrophil number and albumin concentration of bronchoalveolar lavages fluids (BALF) were determined. Besides, bone marrow dendritic cells (BMDCs) were separated and cultured with medium, TSLP, TSLP plus TSLPR-Ig or TSLP plus controlled-Ig. Protein expression levels of TSLP in lung tissues, phosphorylation extracellular regulated protein kinases (pERK) 1/2, p38, and signal transducers and activators of transcription (STAT) 3 in BMDCs were analyzed using Western blotting. Expression of CD40, CD80 and CD86 on pulmonary DCs and BMDCs was determined using flow cytometry (FCM). RESULTS: The W/D ratio, neutrophil number and albumin concentration were significantly decreased in the TSLPR-Ig group compared with the controlled-Ig and model group. Moreover, there was a noticeable decrease in CD40, CD80 or CD86 expression by TSLPR-Ig on both pulmonary DCs and BMDCs. The protein levels of TSLP, pERK1 and STAT3 were significantly decreased by TSLPR-Ig. However, no significant differences were found in p38 and pERK2. CONCLUSION: These results suggest that TSLP may be involved in ALI, and blockage of TSLP signaling using TSLPR-Ig improves ALI at least in part by regulation of DCs functions. The underling downstream signaling mediated by TSLP might be associated with activating the ERK1 and STAT3 signaling pathway. SN - 1936-2625 UR - https://www.unboundmedicine.com/medline/citation/26617780/Blockage_of_thymic_stromal_lymphopoietin_signaling_improves_acute_lung_injury_in_mice_by_regulating_pulmonary_dendritic_cells_ L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/26617780/ DB - PRIME DP - Unbound Medicine ER -