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Neutralization of the neuromuscular inhibition of venom and taipoxin from the taipan (Oxyuranus scutellatus) by F(ab')2 and whole IgG antivenoms.
Toxicol Lett. 2016 Jan 22; 241:175-83.TL

Abstract

The neuromuscular junction activity of Oxyuranus scutellatus venom and its presynaptic neurotoxin, taipoxin, and their neutralization by two antivenoms were examined in mouse phrenic nerve-diaphragm preparations. The action of taipoxin was also studied at 21°C. The efficacy of the antivenoms was also assessed in an in vivo mouse model. Both antivenoms were effective in neutralizing the neuromuscular blocking activity in preincubation-type experiments. In experiments involving independent addition of venom and antivenoms, neutralization depended on the time interval between venom addition and antivenom application. When taipoxin was incubated for 5, 10 or 20min at 21°C, and antivenom added and temperature increased to 37°C, neutralization was achieved only when the toxin was incubated for 5 or 10min. The neutralization by the two antivenoms in an in vivo model showed that both whole IgG and F(ab')2 antivenoms were effective in neutralizing lethality. Our findings highlight the very rapid action of taipan venom at the nerve terminal, and the poor capacity of antivenoms to revert neurotoxicity as the time interval between venom or taipoxin application and antivenom addition increased. Additionally the disparity between molecular masses of the active substances of the two antivenoms did not result in differences in neutralization.

Authors+Show Affiliations

Instituto Clodomiro Picado, Facultad de Microbiología, Universidad de Costa Rica, San José, Costa Rica.Department of Pharmacology, Faculty of Medical Sciences, State University of Campinas (UNICAMP), Campinas, SP, Brazil.Instituto Clodomiro Picado, Facultad de Microbiología, Universidad de Costa Rica, San José, Costa Rica.Instituto Clodomiro Picado, Facultad de Microbiología, Universidad de Costa Rica, San José, Costa Rica.Instituto Clodomiro Picado, Facultad de Microbiología, Universidad de Costa Rica, San José, Costa Rica.Australian Venom Research Unit, Department of Pharmacology and Therapeutics, University of Melbourne, Parkville, Victoria, Australia.Charles Campbell Toxinology Centre, School of Medicine & Health Sciences, University of Papua New Guinea, Port Moresby, Papua New Guinea.Charles Campbell Toxinology Centre, School of Medicine & Health Sciences, University of Papua New Guinea, Port Moresby, Papua New Guinea.Australian Venom Research Unit, Department of Pharmacology and Therapeutics, University of Melbourne, Parkville, Victoria, Australia; Charles Campbell Toxinology Centre, School of Medicine & Health Sciences, University of Papua New Guinea, Port Moresby, Papua New Guinea.Instituto Clodomiro Picado, Facultad de Microbiología, Universidad de Costa Rica, San José, Costa Rica.Australian Venom Research Unit, Department of Pharmacology and Therapeutics, University of Melbourne, Parkville, Victoria, Australia; Charles Campbell Toxinology Centre, School of Medicine & Health Sciences, University of Papua New Guinea, Port Moresby, Papua New Guinea.Department of Pharmacology, Faculty of Medical Sciences, State University of Campinas (UNICAMP), Campinas, SP, Brazil.Instituto Clodomiro Picado, Facultad de Microbiología, Universidad de Costa Rica, San José, Costa Rica. Electronic address: jose.gutierrez@ucr.ac.cr.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26621539

Citation

Herrera, María, et al. "Neutralization of the Neuromuscular Inhibition of Venom and Taipoxin From the Taipan (Oxyuranus Scutellatus) By F(ab')2 and Whole IgG Antivenoms." Toxicology Letters, vol. 241, 2016, pp. 175-83.
Herrera M, de Cássia de O Collaço R, Villalta M, et al. Neutralization of the neuromuscular inhibition of venom and taipoxin from the taipan (Oxyuranus scutellatus) by F(ab')2 and whole IgG antivenoms. Toxicol Lett. 2016;241:175-83.
Herrera, M., de Cássia de O Collaço, R., Villalta, M., Segura, Á., Vargas, M., Wright, C. E., Paiva, O. K., Matainaho, T., Jensen, S. D., León, G., Williams, D. J., Rodrigues-Simioni, L., & Gutiérrez, J. M. (2016). Neutralization of the neuromuscular inhibition of venom and taipoxin from the taipan (Oxyuranus scutellatus) by F(ab')2 and whole IgG antivenoms. Toxicology Letters, 241, 175-83. https://doi.org/10.1016/j.toxlet.2015.11.020
Herrera M, et al. Neutralization of the Neuromuscular Inhibition of Venom and Taipoxin From the Taipan (Oxyuranus Scutellatus) By F(ab')2 and Whole IgG Antivenoms. Toxicol Lett. 2016 Jan 22;241:175-83. PubMed PMID: 26621539.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Neutralization of the neuromuscular inhibition of venom and taipoxin from the taipan (Oxyuranus scutellatus) by F(ab')2 and whole IgG antivenoms. AU - Herrera,María, AU - de Cássia de O Collaço,Rita, AU - Villalta,Mauren, AU - Segura,Álvaro, AU - Vargas,Mariángela, AU - Wright,Christine E, AU - Paiva,Owen K, AU - Matainaho,Teatulohi, AU - Jensen,Simon D, AU - León,Guillermo, AU - Williams,David J, AU - Rodrigues-Simioni,Léa, AU - Gutiérrez,José María, Y1 - 2015/11/24/ PY - 2015/08/24/received PY - 2015/11/05/revised PY - 2015/11/21/accepted PY - 2015/12/2/entrez PY - 2015/12/2/pubmed PY - 2016/4/21/medline KW - Neuromuscular blocking activity KW - Neutralization KW - Phrenic nerve-diaphragm preparation KW - Snake antivenom KW - Taipan venom SP - 175 EP - 83 JF - Toxicology letters JO - Toxicol. Lett. VL - 241 N2 - The neuromuscular junction activity of Oxyuranus scutellatus venom and its presynaptic neurotoxin, taipoxin, and their neutralization by two antivenoms were examined in mouse phrenic nerve-diaphragm preparations. The action of taipoxin was also studied at 21°C. The efficacy of the antivenoms was also assessed in an in vivo mouse model. Both antivenoms were effective in neutralizing the neuromuscular blocking activity in preincubation-type experiments. In experiments involving independent addition of venom and antivenoms, neutralization depended on the time interval between venom addition and antivenom application. When taipoxin was incubated for 5, 10 or 20min at 21°C, and antivenom added and temperature increased to 37°C, neutralization was achieved only when the toxin was incubated for 5 or 10min. The neutralization by the two antivenoms in an in vivo model showed that both whole IgG and F(ab')2 antivenoms were effective in neutralizing lethality. Our findings highlight the very rapid action of taipan venom at the nerve terminal, and the poor capacity of antivenoms to revert neurotoxicity as the time interval between venom or taipoxin application and antivenom addition increased. Additionally the disparity between molecular masses of the active substances of the two antivenoms did not result in differences in neutralization. SN - 1879-3169 UR - https://www.unboundmedicine.com/medline/citation/26621539/Neutralization_of_the_neuromuscular_inhibition_of_venom_and_taipoxin_from_the_taipan__Oxyuranus_scutellatus__by_F_ab'_2_and_whole_IgG_antivenoms_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-4274(15)30116-8 DB - PRIME DP - Unbound Medicine ER -