Tags

Type your tag names separated by a space and hit enter

Antioxidative phytochemicals from Rhododendron oldhamii Maxim. leaf extracts reduce serum uric acid levels in potassium oxonate-induced hyperuricemic mice.
BMC Complement Altern Med. 2015 Dec 01; 15:423.BC

Abstract

BACKGROUND

Some of the genus Rhododendron was used in traditional medicine for arthritis, acute and chronic bronchitis, asthma, pain, inflammation, rheumatism, hypertension and metabolic diseases and many species of the genus Rhododendron contain a large number of phenolic compounds and antioxidant properties that could be developed into pharmaceutical products.

METHODS

In this study, the antioxidative phytochemicals of Rhododendron oldhamii Maxim. leaves were detected by an online HPLC-DPPH method. In addition, the anti-hyperuricemic effect of the active phytochemicals from R. oldhamii leaf extracts was investigated using potassium oxonate (PO)-induced acute hyperuricemia.

RESULTS

Six phytochemicals, including (2R, 3R)-epicatechin (1), (2R, 3R)-taxifolin (2), (2R, 3R)-astilbin (3), hyposide (4), guaijaverin (5), and quercitrin (6), were isolated using the developed screening method. Of these, compounds 3, 4, 5, and 6 were found to be major bioactive phytochemicals, and their contents were determined to be 130.8 ± 10.9, 105.5 ± 8.5, 104.1 ± 4.7, and 108.6 ± 4.0 mg per gram of EtOAc fraction, respectively. In addition, the four major bioactive phytochemicals at the same dosage (100 mmol/kg) were administered to the abdominal cavity of potassium oxonate (PO)-induced hyperuricemic mice, and the serum uric acid level was measured after 3 h of administration. H&E staining showed that PO-induced kidney injury caused renal tubular epithelium nuclear condensation in the cortex areas or the appearance of numerous hyaline casts in the medulla areas; treatment with 100 mmol/kg of EtOAc fraction, (2R, 3R)-astilbin, hyposide, guaijaverin, and quercitrin significantly reduced kidney injury. In addition, the serum uric acid level was significantly suppressed by 54.1, 35.1, 56.3, 56.3, and 53.2 %, respectively, by the administrations of 100 mmol/kg EtOAc fraction and the derived major phytochemicals, (2R, 3R)-astilbin, hyposide, guaijaverin, and quercitrin, compared to the PO group. The administration of 10 mg/kg benzbromarone, a well-known uricosuric agent, significantly reduced the serum uric acid level by 45.5 % compared to the PO group.

CONCLUSION

The in vivo decrease in uric acid was consistent with free radical scavenging activity, indicating that the major phytochemicals of R. oldhamii leave extracts and the derived phytochemicals possess potent hypouricemic effects, and they could be potential candidates for new hypouricemic agents.

Authors+Show Affiliations

Graduate Institute of Sports Science, National Taiwan Sport University, Taoyuan, 333, Taiwan.Department of Forestry and Natural Resources, National Chiayi University, Chiayi, 600, Taiwan.Department of Forestry, National Chung Hsing University, Taichung, 402, Taiwan.Department of Forestry, National Chung Hsing University, Taichung, 402, Taiwan.Department of Forestry, National Chung Hsing University, Taichung, 402, Taiwan.National Laboratory Animal Center, National Applied Research Laboratories, Taipei, 11529, Taiwan.Division of Animal Resource, Animal Technology Laboratories, Agricultural Technology Research Institute, Hsinchu City, 30093, Taiwan.Graduate Institute of Sports Science, National Taiwan Sport University, Taoyuan, 333, Taiwan. d301090007@gmail.com.Department of Forestry, National Chung Hsing University, Taichung, 402, Taiwan. eric@nchu.edu.tw.

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26627882

Citation

Tung, Yu-Tang, et al. "Antioxidative Phytochemicals From Rhododendron Oldhamii Maxim. Leaf Extracts Reduce Serum Uric Acid Levels in Potassium Oxonate-induced Hyperuricemic Mice." BMC Complementary and Alternative Medicine, vol. 15, 2015, p. 423.
Tung YT, Lin LC, Liu YL, et al. Antioxidative phytochemicals from Rhododendron oldhamii Maxim. leaf extracts reduce serum uric acid levels in potassium oxonate-induced hyperuricemic mice. BMC Complement Altern Med. 2015;15:423.
Tung, Y. T., Lin, L. C., Liu, Y. L., Ho, S. T., Lin, C. Y., Chuang, H. L., Chiu, C. C., Huang, C. C., & Wu, J. H. (2015). Antioxidative phytochemicals from Rhododendron oldhamii Maxim. leaf extracts reduce serum uric acid levels in potassium oxonate-induced hyperuricemic mice. BMC Complementary and Alternative Medicine, 15, 423. https://doi.org/10.1186/s12906-015-0950-7
Tung YT, et al. Antioxidative Phytochemicals From Rhododendron Oldhamii Maxim. Leaf Extracts Reduce Serum Uric Acid Levels in Potassium Oxonate-induced Hyperuricemic Mice. BMC Complement Altern Med. 2015 Dec 1;15:423. PubMed PMID: 26627882.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antioxidative phytochemicals from Rhododendron oldhamii Maxim. leaf extracts reduce serum uric acid levels in potassium oxonate-induced hyperuricemic mice. AU - Tung,Yu-Tang, AU - Lin,Lei-Chen, AU - Liu,Ya-Ling, AU - Ho,Shang-Tse, AU - Lin,Chi-Yang, AU - Chuang,Hsiao-Li, AU - Chiu,Chien-Chao, AU - Huang,Chi-Chang, AU - Wu,Jyh-Horng, Y1 - 2015/12/01/ PY - 2015/09/19/received PY - 2015/11/26/accepted PY - 2015/12/3/entrez PY - 2015/12/3/pubmed PY - 2016/4/15/medline SP - 423 EP - 423 JF - BMC complementary and alternative medicine JO - BMC Complement Altern Med VL - 15 N2 - BACKGROUND: Some of the genus Rhododendron was used in traditional medicine for arthritis, acute and chronic bronchitis, asthma, pain, inflammation, rheumatism, hypertension and metabolic diseases and many species of the genus Rhododendron contain a large number of phenolic compounds and antioxidant properties that could be developed into pharmaceutical products. METHODS: In this study, the antioxidative phytochemicals of Rhododendron oldhamii Maxim. leaves were detected by an online HPLC-DPPH method. In addition, the anti-hyperuricemic effect of the active phytochemicals from R. oldhamii leaf extracts was investigated using potassium oxonate (PO)-induced acute hyperuricemia. RESULTS: Six phytochemicals, including (2R, 3R)-epicatechin (1), (2R, 3R)-taxifolin (2), (2R, 3R)-astilbin (3), hyposide (4), guaijaverin (5), and quercitrin (6), were isolated using the developed screening method. Of these, compounds 3, 4, 5, and 6 were found to be major bioactive phytochemicals, and their contents were determined to be 130.8 ± 10.9, 105.5 ± 8.5, 104.1 ± 4.7, and 108.6 ± 4.0 mg per gram of EtOAc fraction, respectively. In addition, the four major bioactive phytochemicals at the same dosage (100 mmol/kg) were administered to the abdominal cavity of potassium oxonate (PO)-induced hyperuricemic mice, and the serum uric acid level was measured after 3 h of administration. H&E staining showed that PO-induced kidney injury caused renal tubular epithelium nuclear condensation in the cortex areas or the appearance of numerous hyaline casts in the medulla areas; treatment with 100 mmol/kg of EtOAc fraction, (2R, 3R)-astilbin, hyposide, guaijaverin, and quercitrin significantly reduced kidney injury. In addition, the serum uric acid level was significantly suppressed by 54.1, 35.1, 56.3, 56.3, and 53.2 %, respectively, by the administrations of 100 mmol/kg EtOAc fraction and the derived major phytochemicals, (2R, 3R)-astilbin, hyposide, guaijaverin, and quercitrin, compared to the PO group. The administration of 10 mg/kg benzbromarone, a well-known uricosuric agent, significantly reduced the serum uric acid level by 45.5 % compared to the PO group. CONCLUSION: The in vivo decrease in uric acid was consistent with free radical scavenging activity, indicating that the major phytochemicals of R. oldhamii leave extracts and the derived phytochemicals possess potent hypouricemic effects, and they could be potential candidates for new hypouricemic agents. SN - 1472-6882 UR - https://www.unboundmedicine.com/medline/citation/26627882/Antioxidative_phytochemicals_from_Rhododendron_oldhamii_Maxim__leaf_extracts_reduce_serum_uric_acid_levels_in_potassium_oxonate_induced_hyperuricemic_mice_ L2 - https://bmccomplementalternmed.biomedcentral.com/articles/10.1186/s12906-015-0950-7 DB - PRIME DP - Unbound Medicine ER -