Tags

Type your tag names separated by a space and hit enter

Molecular and Cytogenetic Characterization of New Wheat-Dasypyrum breviaristatum Derivatives with Post-Harvest Re-Growth Habit.
Genes (Basel). 2015 Nov 27; 6(4):1242-55.G

Abstract

A novel Dasypyrum species, Dasypyrum breviaristatum, serves as a valuable source of useful genes for wheat improvement. The development and characterization of new wheat-D. breviaristatum introgression lines is important to determine the novel gene(s) on specific chromosome(s). We first used multi-color fluorescence in situ hybridization (FISH) to identify the individual D. breviaristatum V(b) chromosomes in a common wheat-D. breviaristatum partial amphiploid, TDH-2. The FISH patterns of D. breviaristatum chromosomes were different from those of D. villosum chromosomes. Lines D2146 and D2150 were selected from a cross between wheat line MY11 and wheat-D. breviaristatum partial amphiploid TDH-2, and they were characterized by FISH and PCR-based molecular markers. We found that D2150 was a monosomic addition line for chromosome 5V(b) of D. breviaristatum, while D2146 had the 5V(b)L chromosome arm translocated with wheat chromosome 5AS. Molecular marker analysis confirmed that the introduced D. breviaristatum chromosome 5V(b)L translocation possessed a duplicated region homoeologous to 5AS, revealing that the 5AS.5V(b)L translocation may not functionally compensate well. The dwarfing and the pre-harvest re-growth habits observed in the wheat-D. breviaristatum chromosome 5V(b) derivatives may be useful for future development of perennial growth wheat lines.

Authors+Show Affiliations

School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China. hongjunzh01@sina.com.School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China. ligr28@uestc.edu.cn.School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China. lidonghaixy@163.com.School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China. gaodangaodan2010@163.com.School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China. christine219@163.com.Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China. yangennian@126.com.School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China. yangzujun@uestc.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26633516

Citation

Zhang, Hongjun, et al. "Molecular and Cytogenetic Characterization of New Wheat-Dasypyrum Breviaristatum Derivatives With Post-Harvest Re-Growth Habit." Genes, vol. 6, no. 4, 2015, pp. 1242-55.
Zhang H, Li G, Li D, et al. Molecular and Cytogenetic Characterization of New Wheat-Dasypyrum breviaristatum Derivatives with Post-Harvest Re-Growth Habit. Genes (Basel). 2015;6(4):1242-55.
Zhang, H., Li, G., Li, D., Gao, D., Zhang, J., Yang, E., & Yang, Z. (2015). Molecular and Cytogenetic Characterization of New Wheat-Dasypyrum breviaristatum Derivatives with Post-Harvest Re-Growth Habit. Genes, 6(4), 1242-55. https://doi.org/10.3390/genes6041242
Zhang H, et al. Molecular and Cytogenetic Characterization of New Wheat-Dasypyrum Breviaristatum Derivatives With Post-Harvest Re-Growth Habit. Genes (Basel). 2015 Nov 27;6(4):1242-55. PubMed PMID: 26633516.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular and Cytogenetic Characterization of New Wheat-Dasypyrum breviaristatum Derivatives with Post-Harvest Re-Growth Habit. AU - Zhang,Hongjun, AU - Li,Guangrong, AU - Li,Donghai, AU - Gao,Dan, AU - Zhang,Jie, AU - Yang,Ennian, AU - Yang,Zujun, Y1 - 2015/11/27/ PY - 2015/08/25/received PY - 2015/11/10/revised PY - 2015/11/18/accepted PY - 2015/12/4/entrez PY - 2015/12/4/pubmed PY - 2015/12/4/medline KW - Dasypyrum breviaristatum KW - fluorescence in situ hybridization KW - molecular markers KW - wheat SP - 1242 EP - 55 JF - Genes JO - Genes (Basel) VL - 6 IS - 4 N2 - A novel Dasypyrum species, Dasypyrum breviaristatum, serves as a valuable source of useful genes for wheat improvement. The development and characterization of new wheat-D. breviaristatum introgression lines is important to determine the novel gene(s) on specific chromosome(s). We first used multi-color fluorescence in situ hybridization (FISH) to identify the individual D. breviaristatum V(b) chromosomes in a common wheat-D. breviaristatum partial amphiploid, TDH-2. The FISH patterns of D. breviaristatum chromosomes were different from those of D. villosum chromosomes. Lines D2146 and D2150 were selected from a cross between wheat line MY11 and wheat-D. breviaristatum partial amphiploid TDH-2, and they were characterized by FISH and PCR-based molecular markers. We found that D2150 was a monosomic addition line for chromosome 5V(b) of D. breviaristatum, while D2146 had the 5V(b)L chromosome arm translocated with wheat chromosome 5AS. Molecular marker analysis confirmed that the introduced D. breviaristatum chromosome 5V(b)L translocation possessed a duplicated region homoeologous to 5AS, revealing that the 5AS.5V(b)L translocation may not functionally compensate well. The dwarfing and the pre-harvest re-growth habits observed in the wheat-D. breviaristatum chromosome 5V(b) derivatives may be useful for future development of perennial growth wheat lines. SN - 2073-4425 UR - https://www.unboundmedicine.com/medline/citation/26633516/Molecular_and_Cytogenetic_Characterization_of_New_Wheat_Dasypyrum_breviaristatum_Derivatives_with_Post_Harvest_Re_Growth_Habit_ L2 - https://www.mdpi.com/resolver?pii=genes6041242 DB - PRIME DP - Unbound Medicine ER -
Try the Free App:
Prime PubMed app for iOS iPhone iPad
Prime PubMed app for Android
Prime PubMed is provided
free to individuals by:
Unbound Medicine.