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Agmatine improves renal function in gentamicin-induced nephrotoxicity in rats.
Can J Physiol Pharmacol. 2016 Mar; 94(3):278-86.CJ

Abstract

The present study was designed to explore the possible protective effects of agmatine, a known nitric oxide (NO) synthase inhibitor, against gentamicin-induced nephrotoxicity in rats. For this purpose, we quantitatively evaluated gentamicin-induced renal structural and functional alterations using histopathological and biochemical approaches. Furthermore, the effect of agmatine on gentamicin-induced hypersensitivity of urinary bladder rings to acetylcholine (ACh) was evaluated. Twenty-four male Wistar albino rats were randomly divided into 3 groups, namely control, gentamicin (100 mg/kg, i.p.), and gentamicin plus agmatine (40 mg/kg, orally). At the end of the study, all rats were sacrificed and then blood and urine samples and kidneys were taken. Administration of agmatine significantly decreased kidney/body mass ratio, serum creatinine, lactate dehydrogenase (LDH), renal malondialdehyde (MDA), myeloperoxidase (MPO), NO, and tumor necrosis factor-alpha (TNF-α) while it significantly increased creatinine clearance and renal superoxide dismutase (SOD) activity when compared with the gentamicin-treated group. Additionally, agmatine ameliorated tissue morphology as evidenced by histological evaluation and reduced the responses of isolated bladder rings to ACh. Our study indicates that agmatine administration with gentamicin attenuates oxidative-stress associated renal injury by reducing oxygen free radicals and lipid peroxidation, restoring NO level and inhibiting inflammatory mediators such as TNF-α.

Authors+Show Affiliations

a Department of Pharmacology and Toxicology, Faculty of Pharmacy, Mansoura University, Mansoura 35516, Egypt.a Department of Pharmacology and Toxicology, Faculty of Pharmacy, Mansoura University, Mansoura 35516, Egypt.b Urology and Nephrology Center, Faculty of Medicine, Mansoura University, Mansoura 35516, Egypt.a Department of Pharmacology and Toxicology, Faculty of Pharmacy, Mansoura University, Mansoura 35516, Egypt.a Department of Pharmacology and Toxicology, Faculty of Pharmacy, Mansoura University, Mansoura 35516, Egypt.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26641937

Citation

El-Kashef, Dalia H., et al. "Agmatine Improves Renal Function in Gentamicin-induced Nephrotoxicity in Rats." Canadian Journal of Physiology and Pharmacology, vol. 94, no. 3, 2016, pp. 278-86.
El-Kashef DH, El-Kenawi AE, Abdel Rahim M, et al. Agmatine improves renal function in gentamicin-induced nephrotoxicity in rats. Can J Physiol Pharmacol. 2016;94(3):278-86.
El-Kashef, D. H., El-Kenawi, A. E., Abdel Rahim, M., Suddek, G. M., & Salem, H. A. (2016). Agmatine improves renal function in gentamicin-induced nephrotoxicity in rats. Canadian Journal of Physiology and Pharmacology, 94(3), 278-86. https://doi.org/10.1139/cjpp-2015-0321
El-Kashef DH, et al. Agmatine Improves Renal Function in Gentamicin-induced Nephrotoxicity in Rats. Can J Physiol Pharmacol. 2016;94(3):278-86. PubMed PMID: 26641937.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Agmatine improves renal function in gentamicin-induced nephrotoxicity in rats. AU - El-Kashef,Dalia H, AU - El-Kenawi,Asmaa E, AU - Abdel Rahim,Mona, AU - Suddek,Ghada M, AU - Salem,Hatem A, Y1 - 2015/08/31/ PY - 2015/12/8/entrez PY - 2015/12/8/pubmed PY - 2016/12/15/medline KW - TNF-α KW - agmatine KW - gentamicin KW - gentamycine KW - nephrotoxicity KW - nitric oxide KW - néphrotoxicité KW - oxyde nitrique KW - urinary bladder KW - vessie SP - 278 EP - 86 JF - Canadian journal of physiology and pharmacology JO - Can J Physiol Pharmacol VL - 94 IS - 3 N2 - The present study was designed to explore the possible protective effects of agmatine, a known nitric oxide (NO) synthase inhibitor, against gentamicin-induced nephrotoxicity in rats. For this purpose, we quantitatively evaluated gentamicin-induced renal structural and functional alterations using histopathological and biochemical approaches. Furthermore, the effect of agmatine on gentamicin-induced hypersensitivity of urinary bladder rings to acetylcholine (ACh) was evaluated. Twenty-four male Wistar albino rats were randomly divided into 3 groups, namely control, gentamicin (100 mg/kg, i.p.), and gentamicin plus agmatine (40 mg/kg, orally). At the end of the study, all rats were sacrificed and then blood and urine samples and kidneys were taken. Administration of agmatine significantly decreased kidney/body mass ratio, serum creatinine, lactate dehydrogenase (LDH), renal malondialdehyde (MDA), myeloperoxidase (MPO), NO, and tumor necrosis factor-alpha (TNF-α) while it significantly increased creatinine clearance and renal superoxide dismutase (SOD) activity when compared with the gentamicin-treated group. Additionally, agmatine ameliorated tissue morphology as evidenced by histological evaluation and reduced the responses of isolated bladder rings to ACh. Our study indicates that agmatine administration with gentamicin attenuates oxidative-stress associated renal injury by reducing oxygen free radicals and lipid peroxidation, restoring NO level and inhibiting inflammatory mediators such as TNF-α. SN - 1205-7541 UR - https://www.unboundmedicine.com/medline/citation/26641937/Agmatine_improves_renal_function_in_gentamicin_induced_nephrotoxicity_in_rats_ L2 - https://cdnsciencepub.com/doi/10.1139/cjpp-2015-0321?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -