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Neuroprotection by Carbenoxolone Against Ischemia Injury Involves PI3K/Akt Pathway.
Clin Lab. 2015; 61(10):1561-8.CL

Abstract

BACKGROUND

Previous experimental studies have shown some protective effects on brain ischemic injures in vivo and in vitro models. However, cotreatment with carbenoxolone (Cbx) and phosatidylinositol 3-kinase (PI3K) inhibitor LY 294002 to a focal cerebral ischemia and reperfusion rat model has not been studied yet. Here we investigate their protective effects on neural cells and examine the function of PI3K/Akt pathway in this protection.

METHODS

Both flow cytometry and western blot were used quantitatively and qualitatively to determine cell apoptosis.

RESULTS

The neural cell apoptosis is related with Cx43, and Bcl-2/Bax and caspase 3 pathways. The percentage of apoptosis cells following transient middle cerebral artery occlusion (MCAO) in mice decrease with the treatment of Cbx. Our data demonstrated that treatment with Cbx reduced cerebral injury in rats exposed to transient focal ischemia and reperfusion (I/R), and this was mediated by the activation of the PI3K/Akt pathways as well as by blocking the caspase 3 apoptosis pathway. LY294002 blocked the increase in phospho-AKT evoked by Cbx and abolished the associated protective effect.

CONCLUSIONS

Taken together, these findings provide evidence that Cbx protects neurons against ischemia injury and this neuroprotective effect involves the PI3K/Akt pathway.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26642720

Citation

Wang, Yu Kai, et al. "Neuroprotection By Carbenoxolone Against Ischemia Injury Involves PI3K/Akt Pathway." Clinical Laboratory, vol. 61, no. 10, 2015, pp. 1561-8.
Wang YK, Deng F, Miao J, et al. Neuroprotection by Carbenoxolone Against Ischemia Injury Involves PI3K/Akt Pathway. Clin Lab. 2015;61(10):1561-8.
Wang, Y. K., Deng, F., Miao, J., Xie, H., & Feng, J. C. (2015). Neuroprotection by Carbenoxolone Against Ischemia Injury Involves PI3K/Akt Pathway. Clinical Laboratory, 61(10), 1561-8.
Wang YK, et al. Neuroprotection By Carbenoxolone Against Ischemia Injury Involves PI3K/Akt Pathway. Clin Lab. 2015;61(10):1561-8. PubMed PMID: 26642720.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Neuroprotection by Carbenoxolone Against Ischemia Injury Involves PI3K/Akt Pathway. AU - Wang,Yu Kai, AU - Deng,Fang, AU - Miao,Jing, AU - Xie,HongYan, AU - Feng,Jia Chun, PY - 2015/12/9/entrez PY - 2015/12/9/pubmed PY - 2015/12/19/medline SP - 1561 EP - 8 JF - Clinical laboratory JO - Clin. Lab. VL - 61 IS - 10 N2 - BACKGROUND: Previous experimental studies have shown some protective effects on brain ischemic injures in vivo and in vitro models. However, cotreatment with carbenoxolone (Cbx) and phosatidylinositol 3-kinase (PI3K) inhibitor LY 294002 to a focal cerebral ischemia and reperfusion rat model has not been studied yet. Here we investigate their protective effects on neural cells and examine the function of PI3K/Akt pathway in this protection. METHODS: Both flow cytometry and western blot were used quantitatively and qualitatively to determine cell apoptosis. RESULTS: The neural cell apoptosis is related with Cx43, and Bcl-2/Bax and caspase 3 pathways. The percentage of apoptosis cells following transient middle cerebral artery occlusion (MCAO) in mice decrease with the treatment of Cbx. Our data demonstrated that treatment with Cbx reduced cerebral injury in rats exposed to transient focal ischemia and reperfusion (I/R), and this was mediated by the activation of the PI3K/Akt pathways as well as by blocking the caspase 3 apoptosis pathway. LY294002 blocked the increase in phospho-AKT evoked by Cbx and abolished the associated protective effect. CONCLUSIONS: Taken together, these findings provide evidence that Cbx protects neurons against ischemia injury and this neuroprotective effect involves the PI3K/Akt pathway. SN - 1433-6510 UR - https://www.unboundmedicine.com/medline/citation/26642720/Neuroprotection_by_Carbenoxolone_Against_Ischemia_Injury_Involves_PI3K/Akt_Pathway_ L2 - https://doi.org/ DB - PRIME DP - Unbound Medicine ER -