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JMJ704 positively regulates rice defense response against Xanthomonas oryzae pv. oryzae infection via reducing H3K4me2/3 associated with negative disease resistance regulators.
BMC Plant Biol 2015; 15:286BP

Abstract

BACKGROUND

Jumonji C (JmjC) domain-containing proteins are a group of functionally conserved histone lysine demethylases in Eukaryotes. Growing evidences have shown that JmjCs epigenetically regulate various biological processes in plants. However, their roles in plant biotic stress, especially in rice bacterial blight resistance have been barely studied so far.

RESULTS

In this study, we found that the global di- and tri-methylation levels on multiple lysine sites of histone three were dramatically altered after being infected by bacterial blight pathogen Xanthomonas oryzae pv. oryzae (Xoo). Xoo infection induced the transcription of 15 JmjCs, suggesting these JmjCs are involved in rice bacterial blight defense. Further functional characterization of JmjC mutants revealed that JMJ704 is a positive regulator of rice bacterial blight resistance as the jmj704 became more susceptible to Xoo than the wild-type. In jmj704, the H3K4me2/3 levels were significantly increased; suggesting JMJ704 may be involved in H3K4me2/3 demethylation. Moreover, JMJ704 suppressed the transcription of the rice defense negative regulator genes, such as NRR, OsWRKY62 and Os-11N3, by reducing the activation marks H3K4me2/3 on them.

CONCLUSIONS

JMJ704 may be a universal switch controlling multiple genes of the bacterial blight resistance pathway. JMJ704 positively regulates rice defense by epigenetically suppressing master negative defense regulators, presenting a novel mechanism distinct from its homolog JMJ705 which also positively regulates rice defense but via activating positive defense regulators.

Authors+Show Affiliations

China National Rice Research Institute, Hangzhou, 311400, China. houyuxuan@caas.cn.School of Agricultural and Food Science, Zhejiang A & F University, Lin'an, 311300, China. wly0121@qq.com.China National Rice Research Institute, Hangzhou, 311400, China. wangling03@caas.cn.China National Rice Research Institute, Hangzhou, 311400, China. liulianmeng@caas.cn.China National Rice Research Institute, Hangzhou, 311400, China. lulu9003@hotmail.com.China National Rice Research Institute, Hangzhou, 311400, China. 925458987@qq.com.School of Agricultural and Food Science, Zhejiang A & F University, Lin'an, 311300, China. qiong.rao@163.com.State key lab of rice biology, China National Rice Research Insititute, Hangzhou, 311400, China. zhangjian@caas.cn.China National Rice Research Institute, Hangzhou, 311400, China. huangshiwen@caas.cn.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26646110

Citation

Hou, Yuxuan, et al. "JMJ704 Positively Regulates Rice Defense Response Against Xanthomonas Oryzae Pv. Oryzae Infection Via Reducing H3K4me2/3 Associated With Negative Disease Resistance Regulators." BMC Plant Biology, vol. 15, 2015, p. 286.
Hou Y, Wang L, Wang L, et al. JMJ704 positively regulates rice defense response against Xanthomonas oryzae pv. oryzae infection via reducing H3K4me2/3 associated with negative disease resistance regulators. BMC Plant Biol. 2015;15:286.
Hou, Y., Wang, L., Wang, L., Liu, L., Li, L., Sun, L., ... Huang, S. (2015). JMJ704 positively regulates rice defense response against Xanthomonas oryzae pv. oryzae infection via reducing H3K4me2/3 associated with negative disease resistance regulators. BMC Plant Biology, 15, p. 286. doi:10.1186/s12870-015-0674-3.
Hou Y, et al. JMJ704 Positively Regulates Rice Defense Response Against Xanthomonas Oryzae Pv. Oryzae Infection Via Reducing H3K4me2/3 Associated With Negative Disease Resistance Regulators. BMC Plant Biol. 2015 Dec 9;15:286. PubMed PMID: 26646110.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - JMJ704 positively regulates rice defense response against Xanthomonas oryzae pv. oryzae infection via reducing H3K4me2/3 associated with negative disease resistance regulators. AU - Hou,Yuxuan, AU - Wang,Liyuan, AU - Wang,Ling, AU - Liu,Lianmeng, AU - Li,Lu, AU - Sun,Lei, AU - Rao,Qiong, AU - Zhang,Jian, AU - Huang,Shiwen, Y1 - 2015/12/09/ PY - 2015/09/06/received PY - 2015/12/03/accepted PY - 2015/12/10/entrez PY - 2015/12/10/pubmed PY - 2016/7/7/medline SP - 286 EP - 286 JF - BMC plant biology JO - BMC Plant Biol. VL - 15 N2 - BACKGROUND: Jumonji C (JmjC) domain-containing proteins are a group of functionally conserved histone lysine demethylases in Eukaryotes. Growing evidences have shown that JmjCs epigenetically regulate various biological processes in plants. However, their roles in plant biotic stress, especially in rice bacterial blight resistance have been barely studied so far. RESULTS: In this study, we found that the global di- and tri-methylation levels on multiple lysine sites of histone three were dramatically altered after being infected by bacterial blight pathogen Xanthomonas oryzae pv. oryzae (Xoo). Xoo infection induced the transcription of 15 JmjCs, suggesting these JmjCs are involved in rice bacterial blight defense. Further functional characterization of JmjC mutants revealed that JMJ704 is a positive regulator of rice bacterial blight resistance as the jmj704 became more susceptible to Xoo than the wild-type. In jmj704, the H3K4me2/3 levels were significantly increased; suggesting JMJ704 may be involved in H3K4me2/3 demethylation. Moreover, JMJ704 suppressed the transcription of the rice defense negative regulator genes, such as NRR, OsWRKY62 and Os-11N3, by reducing the activation marks H3K4me2/3 on them. CONCLUSIONS: JMJ704 may be a universal switch controlling multiple genes of the bacterial blight resistance pathway. JMJ704 positively regulates rice defense by epigenetically suppressing master negative defense regulators, presenting a novel mechanism distinct from its homolog JMJ705 which also positively regulates rice defense but via activating positive defense regulators. SN - 1471-2229 UR - https://www.unboundmedicine.com/medline/citation/26646110/JMJ704_positively_regulates_rice_defense_response_against_Xanthomonas_oryzae_pv__oryzae_infection_via_reducing_H3K4me2/3_associated_with_negative_disease_resistance_regulators_ L2 - https://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-015-0674-3 DB - PRIME DP - Unbound Medicine ER -