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Effect of Fetal Membrane-Derived Mesenchymal Stem Cell Transplantation in Rats With Acute and Chronic Pancreatitis.
Pancreas 2016 May-Jun; 45(5):707-13P

Abstract

OBJECTIVES

Mesenchymal stem cells (MSCs) are a valuable cell source in regenerative medicine and can be isolated from fetal membranes (FMs), particularly amniotic membranes. We investigated the effect of rat FM-derived MSCs (rFM-MSCs) and human amnion-derived MSCs (hAMSCs) on the inflammatory reaction in vitro and therapeutic effects in rats with acute and chronic pancreatitis.

METHODS

Effect of rFM-MSCs or hAMSC-conditioned medium was investigated in vitro. Acute pancreatitis was induced by intraductal injection of 4% taurocholate, and rFM-MSCs were transplanted intravenously. Chronic pancreatitis was induced by intravenous injection of 5 mg/kg dibutyltin dichloride, and hAMSCs were transplanted intravenously.

RESULTS

The inflammatory reaction of macrophages induced by lipopolysaccharide and trypsin was significantly suppressed by rFM-MSC coculture. Pancreatic acinar cell injury induced by cerulein was significantly ameliorated by hAMSC-conditioned medium. Pancreatic stellate cell activation induced by tumor necrosis factor-α was significantly decreased by hAMSC-conditioned medium. Transplantation of rFM-MSCs significantly reduced the histological score and infiltration of CD68-positive macrophages in the rat pancreas. The hAMSC transplantation significantly decreased the expression of MCP-1 and attenuated the downregulation of amylase expression in the pancreas.

CONCLUSIONS

Transplantation of FM-MSCs and AMSCs suppressed the inflammatory reaction of acute and chronic pancreatitis in rats.

Authors+Show Affiliations

From the *Department of Gastroenterology and Hepatology, Graduate School of Medicine, †Laboratory of Pathophysiology and Therapeutics, Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan; and ‡Division of Gastroenterology, Graduate School of Medicine, Tohoku University, Sendai, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26646279

Citation

Kawakubo, Kazumichi, et al. "Effect of Fetal Membrane-Derived Mesenchymal Stem Cell Transplantation in Rats With Acute and Chronic Pancreatitis." Pancreas, vol. 45, no. 5, 2016, pp. 707-13.
Kawakubo K, Ohnishi S, Fujita H, et al. Effect of Fetal Membrane-Derived Mesenchymal Stem Cell Transplantation in Rats With Acute and Chronic Pancreatitis. Pancreas. 2016;45(5):707-13.
Kawakubo, K., Ohnishi, S., Fujita, H., Kuwatani, M., Onishi, R., Masamune, A., ... Sakamoto, N. (2016). Effect of Fetal Membrane-Derived Mesenchymal Stem Cell Transplantation in Rats With Acute and Chronic Pancreatitis. Pancreas, 45(5), pp. 707-13. doi:10.1097/MPA.0000000000000541.
Kawakubo K, et al. Effect of Fetal Membrane-Derived Mesenchymal Stem Cell Transplantation in Rats With Acute and Chronic Pancreatitis. Pancreas. 2016;45(5):707-13. PubMed PMID: 26646279.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of Fetal Membrane-Derived Mesenchymal Stem Cell Transplantation in Rats With Acute and Chronic Pancreatitis. AU - Kawakubo,Kazumichi, AU - Ohnishi,Shunsuke, AU - Fujita,Hirotoshi, AU - Kuwatani,Masaki, AU - Onishi,Reizo, AU - Masamune,Atsushi, AU - Takeda,Hiroshi, AU - Sakamoto,Naoya, PY - 2015/12/10/entrez PY - 2015/12/10/pubmed PY - 2017/1/12/medline SP - 707 EP - 13 JF - Pancreas JO - Pancreas VL - 45 IS - 5 N2 - OBJECTIVES: Mesenchymal stem cells (MSCs) are a valuable cell source in regenerative medicine and can be isolated from fetal membranes (FMs), particularly amniotic membranes. We investigated the effect of rat FM-derived MSCs (rFM-MSCs) and human amnion-derived MSCs (hAMSCs) on the inflammatory reaction in vitro and therapeutic effects in rats with acute and chronic pancreatitis. METHODS: Effect of rFM-MSCs or hAMSC-conditioned medium was investigated in vitro. Acute pancreatitis was induced by intraductal injection of 4% taurocholate, and rFM-MSCs were transplanted intravenously. Chronic pancreatitis was induced by intravenous injection of 5 mg/kg dibutyltin dichloride, and hAMSCs were transplanted intravenously. RESULTS: The inflammatory reaction of macrophages induced by lipopolysaccharide and trypsin was significantly suppressed by rFM-MSC coculture. Pancreatic acinar cell injury induced by cerulein was significantly ameliorated by hAMSC-conditioned medium. Pancreatic stellate cell activation induced by tumor necrosis factor-α was significantly decreased by hAMSC-conditioned medium. Transplantation of rFM-MSCs significantly reduced the histological score and infiltration of CD68-positive macrophages in the rat pancreas. The hAMSC transplantation significantly decreased the expression of MCP-1 and attenuated the downregulation of amylase expression in the pancreas. CONCLUSIONS: Transplantation of FM-MSCs and AMSCs suppressed the inflammatory reaction of acute and chronic pancreatitis in rats. SN - 1536-4828 UR - https://www.unboundmedicine.com/medline/citation/26646279/Effect_of_Fetal_Membrane_Derived_Mesenchymal_Stem_Cell_Transplantation_in_Rats_With_Acute_and_Chronic_Pancreatitis_ L2 - http://Insights.ovid.com/pubmed?pmid=26646279 DB - PRIME DP - Unbound Medicine ER -