Tags

Type your tag names separated by a space and hit enter

Stable Flies (Stomoxys calcitrans L.) from Confined Beef Cattle Do Not Carry Shiga-Toxigenic Escherichia coli (STEC) in the Digestive Tract.
Foodborne Pathog Dis. 2016 Feb; 13(2):65-7.FP

Abstract

BACKGROUND AND OBJECTIVE

Stable flies (Stomoxys calcitrans L.) are very common around confined and pastured cattle, and due to their painful bites they are very important animal pests. Cattle are asymptomatic reservoirs of foodborne pathogens, Escherichia coli O157:H7 and other Shiga-toxigenic E. coli serotypes (STEC). In the present study, the potential of stable flies to carry STEC in a beef cattle feedlot was assessed.

METHODS

Stable flies (n = 180) were collected over 3 summer months and processed individually for STEC-8 that included the serotype O157 and seven non-O157 serotypes (O26, O45, O103, O104, O111, O121, and O145). Isolation and detection of STEC was based on direct plating as well as the enrichment/immunomagnetic separation approach. Modified Posse agar (mP) was used for culturing non-O157 serotypes and sorbitol MacConkey agar with cefixime and potassium tellurite (CT-SMAC) for E. coli O157. Multiplex polymerase chain reactions were used for differentiation of individual serotypes and detection of virulence genes (stx1, stx2, eae, and ehxA).

RESULTS AND CONCLUSIONS

Of 180 stable flies, 67 (37.2%) carried enterics on mP (mean: 3.6 ± 1.05 × 10(6) colony-forming units [CFU]/fly) and 55/180 (30.5%) were positive for bacteria on CT-SMAC (mean: 1.2 ± 1.08 × 10(4) CFU/fly). However, stable flies positive for E. coli serotypes of interest were very rare (prevalence: 1.1%). The three serotype-positive isolates, two E. coli O26 and one E. coli O45, were recovered from two flies and neither of them harbored the virulence genes. We conclude that stable flies likely do not play a role as a biological vector and/or reservoir of STEC-8 in cattle feedlots.

Authors+Show Affiliations

1 Department of Entomology, Kansas State University , Manhattan, Kansas.2 Department of Diagnostic Medicine and Pathobiology, Kansas State University , Manhattan, Kansas.1 Department of Entomology, Kansas State University , Manhattan, Kansas. 2 Department of Diagnostic Medicine and Pathobiology, Kansas State University , Manhattan, Kansas.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

26650821

Citation

Puri-Giri, Rukmini, et al. "Stable Flies (Stomoxys Calcitrans L.) From Confined Beef Cattle Do Not Carry Shiga-Toxigenic Escherichia Coli (STEC) in the Digestive Tract." Foodborne Pathogens and Disease, vol. 13, no. 2, 2016, pp. 65-7.
Puri-Giri R, Ghosh A, Zurek L. Stable Flies (Stomoxys calcitrans L.) from Confined Beef Cattle Do Not Carry Shiga-Toxigenic Escherichia coli (STEC) in the Digestive Tract. Foodborne Pathog Dis. 2016;13(2):65-7.
Puri-Giri, R., Ghosh, A., & Zurek, L. (2016). Stable Flies (Stomoxys calcitrans L.) from Confined Beef Cattle Do Not Carry Shiga-Toxigenic Escherichia coli (STEC) in the Digestive Tract. Foodborne Pathogens and Disease, 13(2), 65-7. https://doi.org/10.1089/fpd.2015.2056
Puri-Giri R, Ghosh A, Zurek L. Stable Flies (Stomoxys Calcitrans L.) From Confined Beef Cattle Do Not Carry Shiga-Toxigenic Escherichia Coli (STEC) in the Digestive Tract. Foodborne Pathog Dis. 2016;13(2):65-7. PubMed PMID: 26650821.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Stable Flies (Stomoxys calcitrans L.) from Confined Beef Cattle Do Not Carry Shiga-Toxigenic Escherichia coli (STEC) in the Digestive Tract. AU - Puri-Giri,Rukmini, AU - Ghosh,Anuradha, AU - Zurek,Ludek, Y1 - 2015/12/09/ PY - 2015/12/10/entrez PY - 2015/12/10/pubmed PY - 2016/11/1/medline SP - 65 EP - 7 JF - Foodborne pathogens and disease JO - Foodborne Pathog Dis VL - 13 IS - 2 N2 - BACKGROUND AND OBJECTIVE: Stable flies (Stomoxys calcitrans L.) are very common around confined and pastured cattle, and due to their painful bites they are very important animal pests. Cattle are asymptomatic reservoirs of foodborne pathogens, Escherichia coli O157:H7 and other Shiga-toxigenic E. coli serotypes (STEC). In the present study, the potential of stable flies to carry STEC in a beef cattle feedlot was assessed. METHODS: Stable flies (n = 180) were collected over 3 summer months and processed individually for STEC-8 that included the serotype O157 and seven non-O157 serotypes (O26, O45, O103, O104, O111, O121, and O145). Isolation and detection of STEC was based on direct plating as well as the enrichment/immunomagnetic separation approach. Modified Posse agar (mP) was used for culturing non-O157 serotypes and sorbitol MacConkey agar with cefixime and potassium tellurite (CT-SMAC) for E. coli O157. Multiplex polymerase chain reactions were used for differentiation of individual serotypes and detection of virulence genes (stx1, stx2, eae, and ehxA). RESULTS AND CONCLUSIONS: Of 180 stable flies, 67 (37.2%) carried enterics on mP (mean: 3.6 ± 1.05 × 10(6) colony-forming units [CFU]/fly) and 55/180 (30.5%) were positive for bacteria on CT-SMAC (mean: 1.2 ± 1.08 × 10(4) CFU/fly). However, stable flies positive for E. coli serotypes of interest were very rare (prevalence: 1.1%). The three serotype-positive isolates, two E. coli O26 and one E. coli O45, were recovered from two flies and neither of them harbored the virulence genes. We conclude that stable flies likely do not play a role as a biological vector and/or reservoir of STEC-8 in cattle feedlots. SN - 1556-7125 UR - https://www.unboundmedicine.com/medline/citation/26650821/Stable_Flies__Stomoxys_calcitrans_L___from_Confined_Beef_Cattle_Do_Not_Carry_Shiga_Toxigenic_Escherichia_coli__STEC__in_the_Digestive_Tract_ L2 - https://www.liebertpub.com/doi/10.1089/fpd.2015.2056?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -