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Antioxidant and immunomodulatory properties of polysaccharides from Allanblackia floribunda Oliv stem bark and Chromolaena odorata (L.) King and H.E. Robins leaves.
BMC Res Notes. 2015 Dec 09; 8:759.BR

Abstract

BACKGROUND

Many plant polysaccharides have shown high antioxidant and immunostimulating properties and can be explored as novel molecules with biological properties that can potentially improve immune function. The objective of this work was to characterize soluble and cell wall polysaccharides isolated from the stem bark of Allanblackia floribunda and Chromolaena odorata leaves and to evaluate their antioxidant and immunomodulatory properties.

METHODS

Three polysaccharide fractions: soluble polysaccharides (PoS), pectins (Pec) and hemicelluloses (Hem) were extracted from A. floribunda stem bark and C. odorata leaves. These samples were analysed for their proteins, phenolic compounds and total sugar contents. The monosaccharide composition was determined by gas chromatography and arabinogalactan proteins content in PoS was evaluated by rocket electrophoresis. The in vitro antioxidant activities were evaluated by 1, 1-diphenyl-2-picryl hydrazyl (DPPH) and 2,2'-azino-bis-3-éthylbenzylthiazoline-6-sulphonic acid (ABTS) radical scavenging assays and ferrous ions chelating activity. Immunomodulatory activities were performed on the peripheral blood mononuclear cells (PBMCs) using proliferation and enzyme linked immunospot (ELISPOT) method to determine the production of an interferon-gamma.

RESULTS

The characterization of the various fractions showed varied metabolites in each plant. In PoS fractions, Ara and Gal were the major monosaccharides found, indicating that arabinogalactans are the primary macromolecules. Hem fractions contained predominantly Xyl and GalA for A. floribunda and Xyl (upto 80 %) for and C. odorata. A. floribunda Hem fraction and C. odorata PoS fraction showed significant DPPH and ABTS radical scavenging activities and immunostimulatory activity via stimulation of PBMC and production of IFN-γ in a dose-dependent manner.

CONCLUSION

The results obtained from this study support the ethnomedicinal use of the stem bark of A. floribunda and leaves of C. odorata. Further research is necessary to have supporting evidence that the antioxidative and immunomodulative activities of these fractions are really connected to the polysaccharides and not polyphenols.

Authors+Show Affiliations

Laboratory of Phytoprotection and Valorisation of Plants Resources, Biotechnology Centre-Nkolbisson, P.O. Box 3851, Messa, Yaounde, Cameroon. boudjeko@yahoo.com. Department of Biochemistry, Faculty of Science, University of Yaounde I, P.O. Box 812, Yaounde, Cameroon. boudjeko@yahoo.com.Department of Animal Biology and Physiology, Faculty of Science, University of Yaounde I, P.O. Box 812, Yaounde, Cameroon. megnekor@yahoo.fr.Department of Biochemistry, Faculty of Science, University of Yaounde I, P.O. Box 812, Yaounde, Cameroon. woguia2002@yahoo.fr.Laboratory of Phytoprotection and Valorisation of Plants Resources, Biotechnology Centre-Nkolbisson, P.O. Box 3851, Messa, Yaounde, Cameroon. Mediessefrancyn@yahoo.fr. Department of Biochemistry, Faculty of Science, University of Yaounde I, P.O. Box 812, Yaounde, Cameroon. Mediessefrancyn@yahoo.fr.Laboratory of Phytoprotection and Valorisation of Plants Resources, Biotechnology Centre-Nkolbisson, P.O. Box 3851, Messa, Yaounde, Cameroon. emerykanemoto@rocketmail.com. Department of Biochemistry, Faculty of Science, University of Yaounde I, P.O. Box 812, Yaounde, Cameroon. emerykanemoto@rocketmail.com.Laboratory of Phytoprotection and Valorisation of Plants Resources, Biotechnology Centre-Nkolbisson, P.O. Box 3851, Messa, Yaounde, Cameroon. daniellenounga@yahoo.fr. Department of Biochemistry, Faculty of Science, University of Yaounde I, P.O. Box 812, Yaounde, Cameroon. daniellenounga@yahoo.fr.Laboratory of Phytoprotection and Valorisation of Plants Resources, Biotechnology Centre-Nkolbisson, P.O. Box 3851, Messa, Yaounde, Cameroon. koumolga@yahoo.fr. Department of Animal Biology and Physiology, Faculty of Science, University of Yaounde I, P.O. Box 812, Yaounde, Cameroon. koumolga@yahoo.fr.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26651330

Citation

Boudjeko, Thaddée, et al. "Antioxidant and Immunomodulatory Properties of Polysaccharides From Allanblackia Floribunda Oliv Stem Bark and Chromolaena Odorata (L.) King and H.E. Robins Leaves." BMC Research Notes, vol. 8, 2015, p. 759.
Boudjeko T, Megnekou R, Woguia AL, et al. Antioxidant and immunomodulatory properties of polysaccharides from Allanblackia floribunda Oliv stem bark and Chromolaena odorata (L.) King and H.E. Robins leaves. BMC Res Notes. 2015;8:759.
Boudjeko, T., Megnekou, R., Woguia, A. L., Kegne, F. M., Ngomoyogoli, J. E., Tchapoum, C. D., & Koum, O. (2015). Antioxidant and immunomodulatory properties of polysaccharides from Allanblackia floribunda Oliv stem bark and Chromolaena odorata (L.) King and H.E. Robins leaves. BMC Research Notes, 8, 759. https://doi.org/10.1186/s13104-015-1703-x
Boudjeko T, et al. Antioxidant and Immunomodulatory Properties of Polysaccharides From Allanblackia Floribunda Oliv Stem Bark and Chromolaena Odorata (L.) King and H.E. Robins Leaves. BMC Res Notes. 2015 Dec 9;8:759. PubMed PMID: 26651330.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antioxidant and immunomodulatory properties of polysaccharides from Allanblackia floribunda Oliv stem bark and Chromolaena odorata (L.) King and H.E. Robins leaves. AU - Boudjeko,Thaddée, AU - Megnekou,Rosette, AU - Woguia,Alice Louise, AU - Kegne,Francine Mediesse, AU - Ngomoyogoli,Judith Emery Kanemoto, AU - Tchapoum,Christiane Danielle Nounga, AU - Koum,Olga, Y1 - 2015/12/09/ PY - 2015/01/23/received PY - 2015/11/17/accepted PY - 2015/12/15/entrez PY - 2015/12/15/pubmed PY - 2016/9/22/medline SP - 759 EP - 759 JF - BMC research notes JO - BMC Res Notes VL - 8 N2 - BACKGROUND: Many plant polysaccharides have shown high antioxidant and immunostimulating properties and can be explored as novel molecules with biological properties that can potentially improve immune function. The objective of this work was to characterize soluble and cell wall polysaccharides isolated from the stem bark of Allanblackia floribunda and Chromolaena odorata leaves and to evaluate their antioxidant and immunomodulatory properties. METHODS: Three polysaccharide fractions: soluble polysaccharides (PoS), pectins (Pec) and hemicelluloses (Hem) were extracted from A. floribunda stem bark and C. odorata leaves. These samples were analysed for their proteins, phenolic compounds and total sugar contents. The monosaccharide composition was determined by gas chromatography and arabinogalactan proteins content in PoS was evaluated by rocket electrophoresis. The in vitro antioxidant activities were evaluated by 1, 1-diphenyl-2-picryl hydrazyl (DPPH) and 2,2'-azino-bis-3-éthylbenzylthiazoline-6-sulphonic acid (ABTS) radical scavenging assays and ferrous ions chelating activity. Immunomodulatory activities were performed on the peripheral blood mononuclear cells (PBMCs) using proliferation and enzyme linked immunospot (ELISPOT) method to determine the production of an interferon-gamma. RESULTS: The characterization of the various fractions showed varied metabolites in each plant. In PoS fractions, Ara and Gal were the major monosaccharides found, indicating that arabinogalactans are the primary macromolecules. Hem fractions contained predominantly Xyl and GalA for A. floribunda and Xyl (upto 80 %) for and C. odorata. A. floribunda Hem fraction and C. odorata PoS fraction showed significant DPPH and ABTS radical scavenging activities and immunostimulatory activity via stimulation of PBMC and production of IFN-γ in a dose-dependent manner. CONCLUSION: The results obtained from this study support the ethnomedicinal use of the stem bark of A. floribunda and leaves of C. odorata. Further research is necessary to have supporting evidence that the antioxidative and immunomodulative activities of these fractions are really connected to the polysaccharides and not polyphenols. SN - 1756-0500 UR - https://www.unboundmedicine.com/medline/citation/26651330/Antioxidant_and_immunomodulatory_properties_of_polysaccharides_from_Allanblackia_floribunda_Oliv_stem_bark_and_Chromolaena_odorata__L___King_and_H_E__Robins_leaves_ L2 - https://bmcresnotes.biomedcentral.com/articles/10.1186/s13104-015-1703-x DB - PRIME DP - Unbound Medicine ER -