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Determination of 11 Cannabinoids in Biomass and Extracts of Different Varieties of Cannabis Using High-Performance Liquid Chromatography.
J AOAC Int 2015 Nov-Dec; 98(6):1523-8JA

Abstract

An HPLC single-laboratory validation was performed for the detection and quantification of the 11 major cannabinoids in most cannabis varieties, namely, cannabidiolic acid (CBDA), cannabigerolic acid (CBGA), cannabigerol (CBG), cannabidiol (CBD), tetrahydrocannabivarin (THCV), cannabinol (CBN), Δ9-trans-tetrahydrocannabinol (Δ9-THC), Δ8-trans-tetrahydrocannabinol (Δ8-THC), cannabicyclol (CBL), cannabichromene (CBC), and Δ9-tetrahydrocannabinolic acid-A (THCAA). The analysis was carried out on the biomass and extracts of these varieties. Methanol-chloroform (9:1, v/v) was used for extraction, 4-androstene-3,17-dione was used as the internal standard, and separation was achieved in 22.2 min on a C18 column using a two- step gradient elution. The method was validated for the 11 cannabinoids. The concentration-response relationship of the method indicated a linear relationship between the concentration and peak area with r2 values of >0.99 for all 11 cannabinoids. Method accuracy was determined through a spike study, and recovery ranged from 89.7 to 105.5% with an RSD of 0.19 to 6.32% for CBDA, CBD, THCV, CBN, Δ9-THC, CBL, CBC, and THCAA; recovery was 84.7, 84.2, and 67.7% for the minor constituents, CBGA, CBG, and Δ8-THC, respectively, with an RSD of 2.58 to 4.96%. The validated method is simple, sensitive, and reproducible and is therefore suitable for the detection and quantification of these cannabinoids in different types of cannabis plant materials.

Authors+Show Affiliations

ElSohly Laboratories, Inc., 5 Industrial Park Dr, Oxford, MS 38655, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

26651563

Citation

Gul, Waseem, et al. "Determination of 11 Cannabinoids in Biomass and Extracts of Different Varieties of Cannabis Using High-Performance Liquid Chromatography." Journal of AOAC International, vol. 98, no. 6, 2015, pp. 1523-8.
Gul W, Gul SW, Radwan MM, et al. Determination of 11 Cannabinoids in Biomass and Extracts of Different Varieties of Cannabis Using High-Performance Liquid Chromatography. J AOAC Int. 2015;98(6):1523-8.
Gul, W., Gul, S. W., Radwan, M. M., Wanas, A. S., Mehmedic, Z., Khan, I. I., ... ElSohly, M. A. (2015). Determination of 11 Cannabinoids in Biomass and Extracts of Different Varieties of Cannabis Using High-Performance Liquid Chromatography. Journal of AOAC International, 98(6), pp. 1523-8. doi:10.5740/jaoacint.15-095.
Gul W, et al. Determination of 11 Cannabinoids in Biomass and Extracts of Different Varieties of Cannabis Using High-Performance Liquid Chromatography. J AOAC Int. 2015;98(6):1523-8. PubMed PMID: 26651563.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of 11 Cannabinoids in Biomass and Extracts of Different Varieties of Cannabis Using High-Performance Liquid Chromatography. AU - Gul,Waseem, AU - Gul,Shahbaz W, AU - Radwan,Mohamed M, AU - Wanas,Amira S, AU - Mehmedic,Zlatko, AU - Khan,Ikhlas I, AU - Sharaf,Maged H M, AU - ElSohly,Mahmoud A, PY - 2015/12/15/entrez PY - 2015/12/15/pubmed PY - 2016/3/8/medline SP - 1523 EP - 8 JF - Journal of AOAC International JO - J AOAC Int VL - 98 IS - 6 N2 - An HPLC single-laboratory validation was performed for the detection and quantification of the 11 major cannabinoids in most cannabis varieties, namely, cannabidiolic acid (CBDA), cannabigerolic acid (CBGA), cannabigerol (CBG), cannabidiol (CBD), tetrahydrocannabivarin (THCV), cannabinol (CBN), Δ9-trans-tetrahydrocannabinol (Δ9-THC), Δ8-trans-tetrahydrocannabinol (Δ8-THC), cannabicyclol (CBL), cannabichromene (CBC), and Δ9-tetrahydrocannabinolic acid-A (THCAA). The analysis was carried out on the biomass and extracts of these varieties. Methanol-chloroform (9:1, v/v) was used for extraction, 4-androstene-3,17-dione was used as the internal standard, and separation was achieved in 22.2 min on a C18 column using a two- step gradient elution. The method was validated for the 11 cannabinoids. The concentration-response relationship of the method indicated a linear relationship between the concentration and peak area with r2 values of >0.99 for all 11 cannabinoids. Method accuracy was determined through a spike study, and recovery ranged from 89.7 to 105.5% with an RSD of 0.19 to 6.32% for CBDA, CBD, THCV, CBN, Δ9-THC, CBL, CBC, and THCAA; recovery was 84.7, 84.2, and 67.7% for the minor constituents, CBGA, CBG, and Δ8-THC, respectively, with an RSD of 2.58 to 4.96%. The validated method is simple, sensitive, and reproducible and is therefore suitable for the detection and quantification of these cannabinoids in different types of cannabis plant materials. SN - 1060-3271 UR - https://www.unboundmedicine.com/medline/citation/26651563/Determination_of_11_Cannabinoids_in_Biomass_and_Extracts_of_Different_Varieties_of_Cannabis_Using_High_Performance_Liquid_Chromatography_ L2 - https://www.ingentaconnect.com/openurl?genre=article&issn=1060-3271&volume=98&issue=6&spage=1523&aulast=Gul DB - PRIME DP - Unbound Medicine ER -