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Rapid Enantiomeric Separation and Quantitation of Levetiracetam on α-Acid Glycoprotein (AGP) Chiral Stationary Phase by High-Performance Liquid Chromatography.
J AOAC Int. 2015 Nov-Dec; 98(6):1529-34.JA

Abstract

A new, simple, and rapid chiral HPLC method was developed for enantioselective analysis of levetiracetam and its enantiomer [(R)-α-ethyl-2- oxo-pyrrolidine acetamide] in a pharmaceutical formulation and bulk material. Enantiomeric separation was achieved on a chiral-α1-acid glycoprotein (AGP) column (150×4.0 mm, 5 μm) using an isocratic mobile phase of phosphate buffer (pH=7) at a flow rate of 0.7 mL/min. The UV detector was set at 210 nm. Calibration curves were linear in the range of 1-100 μg/mL and 0.4-20 μg/mL for levetiracetam and the (R)-enantiomer, respectively. LOD and LOQ for the (R)-enantiomer were 0.1 and 0.4 μg/mL, respectively. The run time of analysis was less than 5.0 min.

Authors+Show Affiliations

Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, PO Box 68149-89468, Khorramabad, Iran.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26651564

Citation

Heydari, Rouhollah, and Mojtaba Shamsipur. "Rapid Enantiomeric Separation and Quantitation of Levetiracetam On α-Acid Glycoprotein (AGP) Chiral Stationary Phase By High-Performance Liquid Chromatography." Journal of AOAC International, vol. 98, no. 6, 2015, pp. 1529-34.
Heydari R, Shamsipur M. Rapid Enantiomeric Separation and Quantitation of Levetiracetam on α-Acid Glycoprotein (AGP) Chiral Stationary Phase by High-Performance Liquid Chromatography. J AOAC Int. 2015;98(6):1529-34.
Heydari, R., & Shamsipur, M. (2015). Rapid Enantiomeric Separation and Quantitation of Levetiracetam on α-Acid Glycoprotein (AGP) Chiral Stationary Phase by High-Performance Liquid Chromatography. Journal of AOAC International, 98(6), 1529-34. https://doi.org/10.5740/jaoacint.15-016
Heydari R, Shamsipur M. Rapid Enantiomeric Separation and Quantitation of Levetiracetam On α-Acid Glycoprotein (AGP) Chiral Stationary Phase By High-Performance Liquid Chromatography. J AOAC Int. 2015 Nov-Dec;98(6):1529-34. PubMed PMID: 26651564.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rapid Enantiomeric Separation and Quantitation of Levetiracetam on α-Acid Glycoprotein (AGP) Chiral Stationary Phase by High-Performance Liquid Chromatography. AU - Heydari,Rouhollah, AU - Shamsipur,Mojtaba, PY - 2015/12/15/entrez PY - 2015/12/15/pubmed PY - 2016/3/8/medline SP - 1529 EP - 34 JF - Journal of AOAC International JO - J AOAC Int VL - 98 IS - 6 N2 - A new, simple, and rapid chiral HPLC method was developed for enantioselective analysis of levetiracetam and its enantiomer [(R)-α-ethyl-2- oxo-pyrrolidine acetamide] in a pharmaceutical formulation and bulk material. Enantiomeric separation was achieved on a chiral-α1-acid glycoprotein (AGP) column (150×4.0 mm, 5 μm) using an isocratic mobile phase of phosphate buffer (pH=7) at a flow rate of 0.7 mL/min. The UV detector was set at 210 nm. Calibration curves were linear in the range of 1-100 μg/mL and 0.4-20 μg/mL for levetiracetam and the (R)-enantiomer, respectively. LOD and LOQ for the (R)-enantiomer were 0.1 and 0.4 μg/mL, respectively. The run time of analysis was less than 5.0 min. SN - 1060-3271 UR - https://www.unboundmedicine.com/medline/citation/26651564/Rapid_Enantiomeric_Separation_and_Quantitation_of_Levetiracetam_on_α_Acid_Glycoprotein__AGP__Chiral_Stationary_Phase_by_High_Performance_Liquid_Chromatography_ L2 - https://www.ingentaconnect.com/openurl?genre=article&issn=1060-3271&volume=98&issue=6&spage=1529&aulast=Heydari DB - PRIME DP - Unbound Medicine ER -