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[Effect of hydrogen-saline on lung injury and heme oxygenase-1 expression in the lung tissue of acute paraquat-intoxicated mice].

Abstract

OBJECTIVE

To investigate the effect of Hydrogen-saline on Lung Injury and HO-1 Expression in The Lung Tissue of Acute Paraquat-intoxicated mice.

METHODS

108 male clean-grade mice were divided randomly into 3 groups: normal control group (n = 8), PQ group (n = 50) and PQ HN group (n = 50), PQ group were lavaged with 20% PQ (50 mg/kg). PQ+Hyclrogen saline group were intxaperitoneal injected with 5 ml/kg saturated hydragen saline after lavaged, 2 times/d. We observe its performance after the poisoning, The lung tissue were taken to HE stained, MDA and SOD activity of lung tissue homogenate were detected, HO-1 activity were observed by immunohistochemistry and western method at 6 h, 1 d, 3 d, 7 d and 14 d.

RESULTS

mice came to shortness of breath, exhaustion and death after poisoned. PQ+hydrogen saline group was more alleviative than PQ group. The lung MDA of PQ group was markedly increased at 6 h and 24 h and SOD was decreased at 6 h, 24 h, 3 d, 7 d, 14 d than normal control group. In Comparison with PQ group, the lung MDA was decreased at 24 h and SOD was increased at 24 h and 3 d (P < 0.05). HO-1 expression trend and distribution in PQ+hydrogen saline group are similar with PQ group, but were significantly higher than that of PQ group and the control group each time point (P < 0.05).

CONCLUSION

Oxidative stress plays important roles in lung injury caused by paraquat. Hydrogen-saline may increase expression of HO-1 and alleviate oxidative stress damage in lung.

Authors+Show Affiliations

Emergency Department of University-Town Hospital of Chongqing Medical University, Chongqing 401331, China.No affiliation info availableE-mail: 565497171@qq.com.No affiliation info available

Pub Type(s)

Journal Article

Language

chi

PubMed ID

26653223

Citation

Liu, Gang, et al. "[Effect of Hydrogen-saline On Lung Injury and Heme Oxygenase-1 Expression in the Lung Tissue of Acute Paraquat-intoxicated Mice]." Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi = Zhonghua Laodong Weisheng Zhiyebing Zazhi = Chinese Journal of Industrial Hygiene and Occupational Diseases, vol. 33, no. 5, 2015, pp. 337-41.
Liu G, Song D, Jiang Y, et al. [Effect of hydrogen-saline on lung injury and heme oxygenase-1 expression in the lung tissue of acute paraquat-intoxicated mice]. Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2015;33(5):337-41.
Liu, G., Song, D., Jiang, Y., & Ge, Y. (2015). [Effect of hydrogen-saline on lung injury and heme oxygenase-1 expression in the lung tissue of acute paraquat-intoxicated mice]. Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi = Zhonghua Laodong Weisheng Zhiyebing Zazhi = Chinese Journal of Industrial Hygiene and Occupational Diseases, 33(5), 337-41.
Liu G, et al. [Effect of Hydrogen-saline On Lung Injury and Heme Oxygenase-1 Expression in the Lung Tissue of Acute Paraquat-intoxicated Mice]. Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2015;33(5):337-41. PubMed PMID: 26653223.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effect of hydrogen-saline on lung injury and heme oxygenase-1 expression in the lung tissue of acute paraquat-intoxicated mice]. AU - Liu,Gang, AU - Song,Dongmei, AU - Jiang,Yu, AU - Ge,Yun, PY - 2015/12/15/entrez PY - 2015/12/15/pubmed PY - 2016/7/13/medline SP - 337 EP - 41 JF - Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases JO - Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi VL - 33 IS - 5 N2 - OBJECTIVE: To investigate the effect of Hydrogen-saline on Lung Injury and HO-1 Expression in The Lung Tissue of Acute Paraquat-intoxicated mice. METHODS: 108 male clean-grade mice were divided randomly into 3 groups: normal control group (n = 8), PQ group (n = 50) and PQ HN group (n = 50), PQ group were lavaged with 20% PQ (50 mg/kg). PQ+Hyclrogen saline group were intxaperitoneal injected with 5 ml/kg saturated hydragen saline after lavaged, 2 times/d. We observe its performance after the poisoning, The lung tissue were taken to HE stained, MDA and SOD activity of lung tissue homogenate were detected, HO-1 activity were observed by immunohistochemistry and western method at 6 h, 1 d, 3 d, 7 d and 14 d. RESULTS: mice came to shortness of breath, exhaustion and death after poisoned. PQ+hydrogen saline group was more alleviative than PQ group. The lung MDA of PQ group was markedly increased at 6 h and 24 h and SOD was decreased at 6 h, 24 h, 3 d, 7 d, 14 d than normal control group. In Comparison with PQ group, the lung MDA was decreased at 24 h and SOD was increased at 24 h and 3 d (P < 0.05). HO-1 expression trend and distribution in PQ+hydrogen saline group are similar with PQ group, but were significantly higher than that of PQ group and the control group each time point (P < 0.05). CONCLUSION: Oxidative stress plays important roles in lung injury caused by paraquat. Hydrogen-saline may increase expression of HO-1 and alleviate oxidative stress damage in lung. SN - 1001-9391 UR - https://www.unboundmedicine.com/medline/citation/26653223/[Effect_of_hydrogen_saline_on_lung_injury_and_heme_oxygenase_1_expression_in_the_lung_tissue_of_acute_paraquat_intoxicated_mice]_ DB - PRIME DP - Unbound Medicine ER -