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Rapid detection of Escherichia coli O157:H7 and Salmonella Typhimurium in foods using an electrochemical immunosensor based on screen-printed interdigitated microelectrode and immunomagnetic separation.
Talanta. 2016; 148:200-8.T

Abstract

Foodborne pathogens have continuously been a serious food safety issue and there is a growing demand for a rapid and sensitive method to screen the pathogens for on-line or in-field applications. Therefore, an impedimetric immunosensor based on the use of magnetic beads (MBs) for separation and a screen-printed interdigitated microelectrode (SP-IDME) for measurement was studied for the rapid detection of Escherichia coli O157:H7 and Salmonella Typhimurium in foods. Streptavidin coated MBs were functionalized with corresponding biotinylated antibodies (Ab) to capture the target bacteria. The glucose oxidase (GOx)-Ab conjugates were employed to label the MBs-Ab-cell complexes. The yielded MBs-Ab-cell-Ab-GOx biomass was mixed with the glucose solution to trigger an enzymatic reaction which produced gluconic acid. This increased the ion strength of the solution, thus decreasing the impedance of the solution measured on the SP-IDME. Our results showed that the immunosensor was capable of specifically detecting E. coli O157:H7 and S. Typhimurium within the range of 10(2)-10(6) cfu ml(-1) in the pure culture samples. E. coli O157:H7 in ground beef and S. Typhimurium in chicken rinse water were also examined. The limits of detection (LODs) for the two bacteria in foods were 2.05×10(3) cfu g(-1) and 1.04×10(3) cfu ml(-1), respectively. This immunosensor required only a bare electrode to measure the impedance changes, and no surficial modification on the electrode was needed. It was low-cost, reproducible, easy-to-operate, and easy-to-preserve. All these merits demonstrated this immunosensor has great potential for the rapid and on-site detection of pathogenic bacteria in foods.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Xu M
Department of Biological and Agricultural Engineering, University of Arkansas, Fayetteville, AR 72701, USA.
Wang R
Department of Biological and Agricultural Engineering, University of Arkansas, Fayetteville, AR 72701, USA.
Li Y
Department of Biological and Agricultural Engineering, University of Arkansas, Fayetteville, AR 72701, USA; Center of Excellence for Poultry Science, University of Arkansas, Fayetteville, AR 72701, USA. Electronic address: yanbinli@uark.edu.

MeSH

AnimalsCattleChickensColony Count, MicrobialElectrochemical TechniquesEscherichia coli O157Food ContaminationHumansImmunomagnetic SeparationMicroelectrodesRed MeatSalmonella typhimuriumTime Factors

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26653441

Citation

Xu, Meng, et al. "Rapid Detection of Escherichia Coli O157:H7 and Salmonella Typhimurium in Foods Using an Electrochemical Immunosensor Based On Screen-printed Interdigitated Microelectrode and Immunomagnetic Separation." Talanta, vol. 148, 2016, pp. 200-8.
Xu M, Wang R, Li Y. Rapid detection of Escherichia coli O157:H7 and Salmonella Typhimurium in foods using an electrochemical immunosensor based on screen-printed interdigitated microelectrode and immunomagnetic separation. Talanta. 2016;148:200-8.
Xu, M., Wang, R., & Li, Y. (2016). Rapid detection of Escherichia coli O157:H7 and Salmonella Typhimurium in foods using an electrochemical immunosensor based on screen-printed interdigitated microelectrode and immunomagnetic separation. Talanta, 148, 200-8. https://doi.org/10.1016/j.talanta.2015.10.082
Xu M, Wang R, Li Y. Rapid Detection of Escherichia Coli O157:H7 and Salmonella Typhimurium in Foods Using an Electrochemical Immunosensor Based On Screen-printed Interdigitated Microelectrode and Immunomagnetic Separation. Talanta. 2016;148:200-8. PubMed PMID: 26653441.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rapid detection of Escherichia coli O157:H7 and Salmonella Typhimurium in foods using an electrochemical immunosensor based on screen-printed interdigitated microelectrode and immunomagnetic separation. AU - Xu,Meng, AU - Wang,Ronghui, AU - Li,Yanbin, Y1 - 2015/10/28/ PY - 2015/8/12/received PY - 2015/10/25/revised PY - 2015/10/26/accepted PY - 2015/12/15/entrez PY - 2015/12/15/pubmed PY - 2016/9/22/medline KW - Electrochemical immunosensor KW - Escherichia coli O157:H7 KW - Magnetic separation KW - Rapid detection KW - Salmonella Typhimurium KW - Screen-printed interdigitated microelectrode SP - 200 EP - 8 JF - Talanta JO - Talanta VL - 148 N2 - Foodborne pathogens have continuously been a serious food safety issue and there is a growing demand for a rapid and sensitive method to screen the pathogens for on-line or in-field applications. Therefore, an impedimetric immunosensor based on the use of magnetic beads (MBs) for separation and a screen-printed interdigitated microelectrode (SP-IDME) for measurement was studied for the rapid detection of Escherichia coli O157:H7 and Salmonella Typhimurium in foods. Streptavidin coated MBs were functionalized with corresponding biotinylated antibodies (Ab) to capture the target bacteria. The glucose oxidase (GOx)-Ab conjugates were employed to label the MBs-Ab-cell complexes. The yielded MBs-Ab-cell-Ab-GOx biomass was mixed with the glucose solution to trigger an enzymatic reaction which produced gluconic acid. This increased the ion strength of the solution, thus decreasing the impedance of the solution measured on the SP-IDME. Our results showed that the immunosensor was capable of specifically detecting E. coli O157:H7 and S. Typhimurium within the range of 10(2)-10(6) cfu ml(-1) in the pure culture samples. E. coli O157:H7 in ground beef and S. Typhimurium in chicken rinse water were also examined. The limits of detection (LODs) for the two bacteria in foods were 2.05×10(3) cfu g(-1) and 1.04×10(3) cfu ml(-1), respectively. This immunosensor required only a bare electrode to measure the impedance changes, and no surficial modification on the electrode was needed. It was low-cost, reproducible, easy-to-operate, and easy-to-preserve. All these merits demonstrated this immunosensor has great potential for the rapid and on-site detection of pathogenic bacteria in foods. SN - 1873-3573 UR - https://www.unboundmedicine.com/medline/citation/26653441/Rapid_detection_of_Escherichia_coli_O157:H7_and_Salmonella_Typhimurium_in_foods_using_an_electrochemical_immunosensor_based_on_screen_printed_interdigitated_microelectrode_and_immunomagnetic_separation_ DB - PRIME DP - Unbound Medicine ER -