Tags

Type your tag names separated by a space and hit enter

[The protective effect of ulinastatin on paraquat-induced injury in HK-2 cells and the underlying mechanisms].

Abstract

OBJECTIVE

To investigate the protective effect of ulinastatin (UTI) on HK-2 cells during paraquat (PQ)-induced injury and its underlying mechanisms.

METHODS

Routinely cultured HK-2 cells were divided into blank control group, PQ group, UTI+PQ group and UTI group. Cell viability was determined by CCK-8 assay. The concentration of PQ in HK-2 cells were measured by high performance liquid chromatography (HPLC). The production of total reactive oxygen species (ROS) were detected by fluorescence microscopy. The activities of superoxide dismutase activity (SOD) and the content of malondialdehyde (MDA) in HK-2 cells were observed by chemical colorimetry. The levels of tumor necrosis factor α (TNF-α) and interleukin 6 (IL-6) were measured by enzyme-linked immunosorbent assay (ELISA).

RESULTS

PQ, even at a dose of 200 µM, could significant suppress the viability of HK-2 cells in a dose-dependent and time-dependent. UTI showed no significant inhibitory effect on the viability of HK-2 cells when given at a dose below 8 000 U/ml (P > 0.05). Compared with the PQ group, the UTI+PQ group had significantly increased the viability of HK-2 cells in a dose-dependent of UTI (P < 0.05). Compared with the PQ group on the same hour, the UTI+PQ group showed decreased in PQ concentration in HK-2 cells (P < 0.05 for all except 6 h). Compared with the blank control group, the PQ group had significantly decreased SOD activity and significantly increased ROS level and MDA content (P < 0.05). Compared with the PQ group, the UTI+PQ group had significantly increased SOD activity and significantly decreased ROS level and MDA content (P < 0.05). Compared with the blank control group, the PQ group had significantly increased IL-6 and TNF-α level (P < 0.05); Compared with the PQ group, the UTI+PQ group had significantly decreased IL-6 and TNF-α level (P < 0.05).

CONCLUSION

UTI significantly reduces the PQ-induced oxidative damage and inflammatory injury and its mechanism may be by reducing the accumulation of PQ in HK-2 cells.

Authors+Show Affiliations

Emergency Department of the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China.Emergency Department of the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China.Emergency Department of the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China.Emergency Department of the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China.Emergency Department of the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China.Emergency Department of the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China; E-mial: lzq640815@163.com.

Pub Type(s)

Journal Article

Language

chi

PubMed ID

26653645

Citation

She, Xingrong, et al. "[The Protective Effect of Ulinastatin On Paraquat-induced Injury in HK-2 Cells and the Underlying Mechanisms]." Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi = Zhonghua Laodong Weisheng Zhiyebing Zazhi = Chinese Journal of Industrial Hygiene and Occupational Diseases, vol. 33, no. 7, 2015, pp. 501-6.
She X, Hong G, Tan J, et al. [The protective effect of ulinastatin on paraquat-induced injury in HK-2 cells and the underlying mechanisms]. Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2015;33(7):501-6.
She, X., Hong, G., Tan, J., Zhao, G., Li, M., & Lu, Z. (2015). [The protective effect of ulinastatin on paraquat-induced injury in HK-2 cells and the underlying mechanisms]. Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi = Zhonghua Laodong Weisheng Zhiyebing Zazhi = Chinese Journal of Industrial Hygiene and Occupational Diseases, 33(7), 501-6.
She X, et al. [The Protective Effect of Ulinastatin On Paraquat-induced Injury in HK-2 Cells and the Underlying Mechanisms]. Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2015;33(7):501-6. PubMed PMID: 26653645.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [The protective effect of ulinastatin on paraquat-induced injury in HK-2 cells and the underlying mechanisms]. AU - She,Xingrong, AU - Hong,Guangliang, AU - Tan,Jiaping, AU - Zhao,Guangju, AU - Li,Mengfang, AU - Lu,Zhongqiu, PY - 2015/12/15/entrez PY - 2015/12/15/pubmed PY - 2016/7/13/medline SP - 501 EP - 6 JF - Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases JO - Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi VL - 33 IS - 7 N2 - OBJECTIVE: To investigate the protective effect of ulinastatin (UTI) on HK-2 cells during paraquat (PQ)-induced injury and its underlying mechanisms. METHODS: Routinely cultured HK-2 cells were divided into blank control group, PQ group, UTI+PQ group and UTI group. Cell viability was determined by CCK-8 assay. The concentration of PQ in HK-2 cells were measured by high performance liquid chromatography (HPLC). The production of total reactive oxygen species (ROS) were detected by fluorescence microscopy. The activities of superoxide dismutase activity (SOD) and the content of malondialdehyde (MDA) in HK-2 cells were observed by chemical colorimetry. The levels of tumor necrosis factor α (TNF-α) and interleukin 6 (IL-6) were measured by enzyme-linked immunosorbent assay (ELISA). RESULTS: PQ, even at a dose of 200 µM, could significant suppress the viability of HK-2 cells in a dose-dependent and time-dependent. UTI showed no significant inhibitory effect on the viability of HK-2 cells when given at a dose below 8 000 U/ml (P > 0.05). Compared with the PQ group, the UTI+PQ group had significantly increased the viability of HK-2 cells in a dose-dependent of UTI (P < 0.05). Compared with the PQ group on the same hour, the UTI+PQ group showed decreased in PQ concentration in HK-2 cells (P < 0.05 for all except 6 h). Compared with the blank control group, the PQ group had significantly decreased SOD activity and significantly increased ROS level and MDA content (P < 0.05). Compared with the PQ group, the UTI+PQ group had significantly increased SOD activity and significantly decreased ROS level and MDA content (P < 0.05). Compared with the blank control group, the PQ group had significantly increased IL-6 and TNF-α level (P < 0.05); Compared with the PQ group, the UTI+PQ group had significantly decreased IL-6 and TNF-α level (P < 0.05). CONCLUSION: UTI significantly reduces the PQ-induced oxidative damage and inflammatory injury and its mechanism may be by reducing the accumulation of PQ in HK-2 cells. SN - 1001-9391 UR - https://www.unboundmedicine.com/medline/citation/26653645/[The_protective_effect_of_ulinastatin_on_paraquat_induced_injury_in_HK_2_cells_and_the_underlying_mechanisms]_ L2 - https://antibodies.cancer.gov/detail/CPTC-IL6-1 DB - PRIME DP - Unbound Medicine ER -