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The 1918 Influenza Virus PB2 Protein Enhances Virulence through the Disruption of Inflammatory and Wnt-Mediated Signaling in Mice.
J Virol. 2015 Dec 09; 90(5):2240-53.JV

Abstract

The 1918-1919 influenza pandemic remains the single greatest infectious disease outbreak in the past century. Mouse and nonhuman primate infection models have shown that the 1918 virus induces overly aggressive innate and proinflammatory responses. To understand the response to viral infection and the role of individual 1918 genes on the host response to the 1918 virus, we examined reassortant avian viruses nearly identical to the pandemic 1918 virus (1918-like avian virus) carrying either the 1918 hemagglutinin (HA) or PB2 gene. In mice, both genes enhanced 1918-like avian virus replication, but only the mammalian host adaptation of the 1918-like avian virus through reassortment of the 1918 PB2 led to increased lethality. Through the combination of viral genetics and host transcriptional profiling, we provide a multidimensional view of the molecular mechanisms by which the 1918 PB2 gene drives viral pathogenicity. We demonstrate that 1918 PB2 enhances immune and inflammatory responses concomitant with increased cellular infiltration in the lung. We also show for the first time, that 1918 PB2 expression results in the repression of both canonical and noncanonical Wnt signaling pathways, which are crucial for inflammation-mediated lung regeneration and repair. Finally, we utilize regulatory enrichment and network analysis to define the molecular regulators of inflammation, epithelial regeneration, and lung immunopathology that are dysregulated during influenza virus infection. Taken together, our data suggest that while both HA and PB2 are important for viral replication, only 1918 PB2 exacerbates lung damage in mice infected with a reassortant 1918-like avian virus.

IMPORTANCE

As viral pathogenesis is determined in part by the host response, understanding the key host molecular driver(s) of virus-mediated disease, in relation to individual viral genes, is a promising approach to host-oriented drug efforts in preventing disease. Previous studies have demonstrated the importance of host adaptive genes, HA and PB2, in mediating disease although the mechanisms by which they do so are still poorly understood. Here, we combine viral genetics and host transcriptional profiling to show that although both 1918 HA and 1918 PB2 are important mediators of efficient viral replication, only 1918 PB2 impacts the pathogenicity of an avian influenza virus sharing high homology to the 1918 pandemic influenza virus. We demonstrate that 1918 PB2 enhances deleterious inflammatory responses and the inhibition of regeneration and repair functions coordinated by Wnt signaling in the lungs of infected mice, thereby promoting virus-associated disease.

Authors+Show Affiliations

Department of Microbiology, University of Washington, Seattle, Washington, USA.Department of Microbiology, University of Washington, Seattle, Washington, USA.Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, Wisconsin, USA.Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, Wisconsin, USA.Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, Wisconsin, USA.Department of Microbiology, University of Washington, Seattle, Washington, USA.Department of Microbiology, University of Washington, Seattle, Washington, USA.Department of Microbiology, University of Washington, Seattle, Washington, USA.Department of Microbiology, University of Washington, Seattle, Washington, USA.Department of Microbiology, University of Washington, Seattle, Washington, USA.Howard Hughes Medical Institute, Institute for Stem Cell and Regenerative Medicine, and Department of Pharmacology, University of Washington School of Medicine, Seattle, Washington, USA.Howard Hughes Medical Institute, Institute for Stem Cell and Regenerative Medicine, and Department of Pharmacology, University of Washington School of Medicine, Seattle, Washington, USA.Laboratory of Virology, National Influenza Centre (South of France), Hospices Civils de Lyon, Bron, France, and Laboratorie Virpath EA4610, Université Claude Bernard Lyon 1, Lyon, France.Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, Wisconsin, USA.Department of Microbiology, University of Washington, Seattle, Washington, USA Washington National Primate Research Center, University of Washington, Seattle, Washington, USA honey@uw.edu.

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, N.I.H., Intramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26656717

Citation

Forero, Adriana, et al. "The 1918 Influenza Virus PB2 Protein Enhances Virulence Through the Disruption of Inflammatory and Wnt-Mediated Signaling in Mice." Journal of Virology, vol. 90, no. 5, 2015, pp. 2240-53.
Forero A, Tisoncik-Go J, Watanabe T, et al. The 1918 Influenza Virus PB2 Protein Enhances Virulence through the Disruption of Inflammatory and Wnt-Mediated Signaling in Mice. J Virol. 2015;90(5):2240-53.
Forero, A., Tisoncik-Go, J., Watanabe, T., Zhong, G., Hatta, M., Tchitchek, N., Selinger, C., Chang, J., Barker, K., Morrison, J., Berndt, J. D., Moon, R. T., Josset, L., Kawaoka, Y., & Katze, M. G. (2015). The 1918 Influenza Virus PB2 Protein Enhances Virulence through the Disruption of Inflammatory and Wnt-Mediated Signaling in Mice. Journal of Virology, 90(5), 2240-53. https://doi.org/10.1128/JVI.02974-15
Forero A, et al. The 1918 Influenza Virus PB2 Protein Enhances Virulence Through the Disruption of Inflammatory and Wnt-Mediated Signaling in Mice. J Virol. 2015 Dec 9;90(5):2240-53. PubMed PMID: 26656717.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The 1918 Influenza Virus PB2 Protein Enhances Virulence through the Disruption of Inflammatory and Wnt-Mediated Signaling in Mice. AU - Forero,Adriana, AU - Tisoncik-Go,Jennifer, AU - Watanabe,Tokiko, AU - Zhong,Gongxun, AU - Hatta,Masato, AU - Tchitchek,Nicolas, AU - Selinger,Christian, AU - Chang,Jean, AU - Barker,Kristi, AU - Morrison,Juliet, AU - Berndt,Jason D, AU - Moon,Randall T, AU - Josset,Laurence, AU - Kawaoka,Yoshihiro, AU - Katze,Michael G, Y1 - 2015/12/09/ PY - 2015/11/23/received PY - 2015/12/01/accepted PY - 2015/12/15/entrez PY - 2015/12/15/pubmed PY - 2016/6/22/medline SP - 2240 EP - 53 JF - Journal of virology JO - J. Virol. VL - 90 IS - 5 N2 - UNLABELLED: The 1918-1919 influenza pandemic remains the single greatest infectious disease outbreak in the past century. Mouse and nonhuman primate infection models have shown that the 1918 virus induces overly aggressive innate and proinflammatory responses. To understand the response to viral infection and the role of individual 1918 genes on the host response to the 1918 virus, we examined reassortant avian viruses nearly identical to the pandemic 1918 virus (1918-like avian virus) carrying either the 1918 hemagglutinin (HA) or PB2 gene. In mice, both genes enhanced 1918-like avian virus replication, but only the mammalian host adaptation of the 1918-like avian virus through reassortment of the 1918 PB2 led to increased lethality. Through the combination of viral genetics and host transcriptional profiling, we provide a multidimensional view of the molecular mechanisms by which the 1918 PB2 gene drives viral pathogenicity. We demonstrate that 1918 PB2 enhances immune and inflammatory responses concomitant with increased cellular infiltration in the lung. We also show for the first time, that 1918 PB2 expression results in the repression of both canonical and noncanonical Wnt signaling pathways, which are crucial for inflammation-mediated lung regeneration and repair. Finally, we utilize regulatory enrichment and network analysis to define the molecular regulators of inflammation, epithelial regeneration, and lung immunopathology that are dysregulated during influenza virus infection. Taken together, our data suggest that while both HA and PB2 are important for viral replication, only 1918 PB2 exacerbates lung damage in mice infected with a reassortant 1918-like avian virus. IMPORTANCE: As viral pathogenesis is determined in part by the host response, understanding the key host molecular driver(s) of virus-mediated disease, in relation to individual viral genes, is a promising approach to host-oriented drug efforts in preventing disease. Previous studies have demonstrated the importance of host adaptive genes, HA and PB2, in mediating disease although the mechanisms by which they do so are still poorly understood. Here, we combine viral genetics and host transcriptional profiling to show that although both 1918 HA and 1918 PB2 are important mediators of efficient viral replication, only 1918 PB2 impacts the pathogenicity of an avian influenza virus sharing high homology to the 1918 pandemic influenza virus. We demonstrate that 1918 PB2 enhances deleterious inflammatory responses and the inhibition of regeneration and repair functions coordinated by Wnt signaling in the lungs of infected mice, thereby promoting virus-associated disease. SN - 1098-5514 UR - https://www.unboundmedicine.com/medline/citation/26656717/The_1918_Influenza_Virus_PB2_Protein_Enhances_Virulence_through_the_Disruption_of_Inflammatory_and_Wnt_Mediated_Signaling_in_Mice_ L2 - http://jvi.asm.org/cgi/pmidlookup?view=long&pmid=26656717 DB - PRIME DP - Unbound Medicine ER -