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Activation of the IGF1 pathway mediates changes in cellular contractility and motility in single-suture craniosynostosis.
J Cell Sci 2016; 129(3):483-91JC

Abstract

Insulin growth factor 1 (IGF1) is a major anabolic signal that is essential during skeletal development, cellular adhesion and migration. Recent transcriptomic studies have shown that there is an upregulation in IGF1 expression in calvarial osteoblasts derived from patients with single-suture craniosynostosis (SSC). Upregulation of the IGF1 signaling pathway is known to induce increased expression of a set of osteogenic markers that previously have been shown to be correlated with contractility and migration. Although the IGF1 signaling pathway has been implicated in SSC, a correlation between IGF1, contractility and migration has not yet been investigated. Here, we examined the effect of IGF1 activation in inducing cellular contractility and migration in SSC osteoblasts using micropost arrays and time-lapse microscopy. We observed that the contractile forces and migration speeds of SSC osteoblasts correlated with IGF1 expression. Moreover, both contractility and migration of SSC osteoblasts were directly affected by the interaction of IGF1 with IGF1 receptor (IGF1R). Our results suggest that IGF1 activity can provide valuable insight for phenotype-genotype correlation in SSC osteoblasts and might provide a target for therapeutic intervention.

Authors+Show Affiliations

Department of Mechanical Engineering, University of Washington, Seattle, WA 98195, USA Seattle Children's Research Institute, Center for Developmental Biology and Regenerative Medicine, Seattle, WA 98101, USA.Department of Genome Sciences, University of Washington, Seattle, WA 98105, USA.Department of Mechanical Engineering, University of Washington, Seattle, WA 98195, USA.Department of Bioengineering, University of Washington, Seattle, WA 98105, USA.Department of Mechanical Engineering, University of Washington, Seattle, WA 98195, USA.Department of Genome Sciences, University of Washington, Seattle, WA 98105, USA.Department of Genome Sciences, University of Washington, Seattle, WA 98105, USA.Seattle Children's Research Institute, Center for Developmental Biology and Regenerative Medicine, Seattle, WA 98101, USA.Seattle Children's Research Institute, Center for Developmental Biology and Regenerative Medicine, Seattle, WA 98101, USA.Seattle Children's Research Institute, Center for Developmental Biology and Regenerative Medicine, Seattle, WA 98101, USA.Seattle Children's Research Institute, Center for Developmental Biology and Regenerative Medicine, Seattle, WA 98101, USA Division of Craniofacial Medicine and the Department of Pediatrics, University of Washington, Seattle, WA 98105, USA.Department of Mechanical Engineering, University of Washington, Seattle, WA 98195, USA Department of Bioengineering, University of Washington, Seattle, WA 98105, USA nsniadec@uw.edu.

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

26659664

Citation

Al-Rekabi, Zeinab, et al. "Activation of the IGF1 Pathway Mediates Changes in Cellular Contractility and Motility in Single-suture Craniosynostosis." Journal of Cell Science, vol. 129, no. 3, 2016, pp. 483-91.
Al-Rekabi Z, Wheeler MM, Leonard A, et al. Activation of the IGF1 pathway mediates changes in cellular contractility and motility in single-suture craniosynostosis. J Cell Sci. 2016;129(3):483-91.
Al-Rekabi, Z., Wheeler, M. M., Leonard, A., Fura, A. M., Juhlin, I., Frazar, C., ... Sniadecki, N. J. (2016). Activation of the IGF1 pathway mediates changes in cellular contractility and motility in single-suture craniosynostosis. Journal of Cell Science, 129(3), pp. 483-91. doi:10.1242/jcs.175976.
Al-Rekabi Z, et al. Activation of the IGF1 Pathway Mediates Changes in Cellular Contractility and Motility in Single-suture Craniosynostosis. J Cell Sci. 2016 Feb 1;129(3):483-91. PubMed PMID: 26659664.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Activation of the IGF1 pathway mediates changes in cellular contractility and motility in single-suture craniosynostosis. AU - Al-Rekabi,Zeinab, AU - Wheeler,Marsha M, AU - Leonard,Andrea, AU - Fura,Adriane M, AU - Juhlin,Ilsa, AU - Frazar,Christopher, AU - Smith,Joshua D, AU - Park,Sarah S, AU - Gustafson,Jennifer A, AU - Clarke,Christine M, AU - Cunningham,Michael L, AU - Sniadecki,Nathan J, Y1 - 2015/12/11/ PY - 2015/06/17/received PY - 2015/12/06/accepted PY - 2015/12/15/entrez PY - 2015/12/15/pubmed PY - 2016/10/27/medline KW - Calvarial osteoblast KW - Cell migration KW - IGF1 KW - IGF1R KW - Single-suture craniosynostosis KW - Traction force SP - 483 EP - 91 JF - Journal of cell science JO - J. Cell. Sci. VL - 129 IS - 3 N2 - Insulin growth factor 1 (IGF1) is a major anabolic signal that is essential during skeletal development, cellular adhesion and migration. Recent transcriptomic studies have shown that there is an upregulation in IGF1 expression in calvarial osteoblasts derived from patients with single-suture craniosynostosis (SSC). Upregulation of the IGF1 signaling pathway is known to induce increased expression of a set of osteogenic markers that previously have been shown to be correlated with contractility and migration. Although the IGF1 signaling pathway has been implicated in SSC, a correlation between IGF1, contractility and migration has not yet been investigated. Here, we examined the effect of IGF1 activation in inducing cellular contractility and migration in SSC osteoblasts using micropost arrays and time-lapse microscopy. We observed that the contractile forces and migration speeds of SSC osteoblasts correlated with IGF1 expression. Moreover, both contractility and migration of SSC osteoblasts were directly affected by the interaction of IGF1 with IGF1 receptor (IGF1R). Our results suggest that IGF1 activity can provide valuable insight for phenotype-genotype correlation in SSC osteoblasts and might provide a target for therapeutic intervention. SN - 1477-9137 UR - https://www.unboundmedicine.com/medline/citation/26659664/Activation_of_the_IGF1_pathway_mediates_changes_in_cellular_contractility_and_motility_in_single_suture_craniosynostosis_ L2 - http://jcs.biologists.org/cgi/pmidlookup?view=long&pmid=26659664 DB - PRIME DP - Unbound Medicine ER -