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[Effects of Electroacupunctrue Combined with Dietary Control on Peroxisome Proliferator-activa- ted Receptor-α, and Liver Fatty Acid-binding Protein Levels in Non-alcoholic Fatty Liver Disease Rats].
Zhen Ci Yan Jiu. 2015 Oct; 40(5):345-51.ZC

Abstract

OBJECTIVE

To observe the effect of electroacupunctrue (EA) intervention or EA combined with dietary control on peroxisome proliferator-activated receptor (PPAR)-α, and liver fatty acid-binding protein (L-FABP) levels in non-alcoholic fatty liver disease (NAFLD) rats, so as to reveal its mechanism underlying improvement of NAFLD.

METHODS

Sixty SD male rats were randomly divided into common diet (control) group (n = 10) and high-fat diet group (n = 45). The NAFLD model was established by feeding the animals with high-fat forage (HFF, including cholesterol, sodium cholate, propylthiouracil, sucrose, lard and common forage) for 5 weeks. Forty NAFLD rats were then randomized into model, EA + HFF, low-fat forage (LFF) and EA+ LFF groups (n = 10 rats in each group). EA (4 Hz/20 Hz, 3 mA) was applied to ipsilateral "Zusanli" (ST 36),"Sanyinjiao" (SP 6) and "Taichong" (LR 3) for 20 min, once daily for 4 weeks. The pathologic changes of the hepatic tissue were detected by H. E. staining. Serum total cholesterol (TC) and triglyceride (TG) contents were determined by using enzymatic methods, serum free fat acids (FFA) content was detected by colorimetry. The expression levels of PPAR-α and L-FABP protein and gene of the liver tissue were determined by Western blot and RT-PCR, respectively.

RESULTS

H. E. staining showed that the hepatocytes presented moderate or severe bullous adipose degeneration in rats of the model group, vesicular steatosis in the EA + HFF and LFF groups, turned to almost normal but with small amount of lipid droplets in the EA + LFF group. The contents of serum TC, TG and FFA were significantly higher in the model group than in the control group (P < 0.05), and were obviously decreased in the EA + HFF, LFF and EA + LFF groups in comparison with the model group (P < 0.05). Compared to the control group, hepatic PPAR-α protein and mRNA were markedly down-regulated in the model group, and hepatic L-FABP protein and mRNA considerably up-regulated in the model group (P < 0.05). Following EA intervention and low fat diet feeding, PPAR-α protein and mRNA were markedly up-regulated while L-FABP protein and mRNA remarkably down-regulated in the EA + HFF, LFF and EA + LFF groups (P < 0.05).

CONCLUSION

EA intervention or EA combined with dietary control can relieve hepatic pathological changes in NAFLD rats, which may be related to its effects in down-regulating blood lipid level and hepatic L-FABP protein and mRNA expression and in up-regulating PPAR-α protein and mRNA expression, and the effect of combined treatment was relatively better than simple EA or LFF treatment.

Authors

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Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

26669188

Citation

Zhang, Yi, et al. "[Effects of Electroacupunctrue Combined With Dietary Control On Peroxisome Proliferator-activa- Ted Receptor-α, and Liver Fatty Acid-binding Protein Levels in Non-alcoholic Fatty Liver Disease Rats]." Zhen Ci Yan Jiu = Acupuncture Research, vol. 40, no. 5, 2015, pp. 345-51.
Zhang Y, Tang CL, Tian Y, et al. [Effects of Electroacupunctrue Combined with Dietary Control on Peroxisome Proliferator-activa- ted Receptor-α, and Liver Fatty Acid-binding Protein Levels in Non-alcoholic Fatty Liver Disease Rats]. Zhen Ci Yan Jiu. 2015;40(5):345-51.
Zhang, Y., Tang, C. L., Tian, Y., Yuan, H. Z., Yang, H., Tang, N. Z., Gao, R. Q., & Cao, J. (2015). [Effects of Electroacupunctrue Combined with Dietary Control on Peroxisome Proliferator-activa- ted Receptor-α, and Liver Fatty Acid-binding Protein Levels in Non-alcoholic Fatty Liver Disease Rats]. Zhen Ci Yan Jiu = Acupuncture Research, 40(5), 345-51.
Zhang Y, et al. [Effects of Electroacupunctrue Combined With Dietary Control On Peroxisome Proliferator-activa- Ted Receptor-α, and Liver Fatty Acid-binding Protein Levels in Non-alcoholic Fatty Liver Disease Rats]. Zhen Ci Yan Jiu. 2015;40(5):345-51. PubMed PMID: 26669188.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effects of Electroacupunctrue Combined with Dietary Control on Peroxisome Proliferator-activa- ted Receptor-α, and Liver Fatty Acid-binding Protein Levels in Non-alcoholic Fatty Liver Disease Rats]. AU - Zhang,Yi, AU - Tang,Cheng-lin, AU - Tian,Yuan, AU - Yuan,Hai-zhou, AU - Yang,Hui, AU - Tang,Nian-zhen, AU - Gao,Rui-qi, AU - Cao,Jing, PY - 2015/12/17/entrez PY - 2015/12/17/pubmed PY - 2016/2/10/medline SP - 345 EP - 51 JF - Zhen ci yan jiu = Acupuncture research JO - Zhen Ci Yan Jiu VL - 40 IS - 5 N2 - OBJECTIVE: To observe the effect of electroacupunctrue (EA) intervention or EA combined with dietary control on peroxisome proliferator-activated receptor (PPAR)-α, and liver fatty acid-binding protein (L-FABP) levels in non-alcoholic fatty liver disease (NAFLD) rats, so as to reveal its mechanism underlying improvement of NAFLD. METHODS: Sixty SD male rats were randomly divided into common diet (control) group (n = 10) and high-fat diet group (n = 45). The NAFLD model was established by feeding the animals with high-fat forage (HFF, including cholesterol, sodium cholate, propylthiouracil, sucrose, lard and common forage) for 5 weeks. Forty NAFLD rats were then randomized into model, EA + HFF, low-fat forage (LFF) and EA+ LFF groups (n = 10 rats in each group). EA (4 Hz/20 Hz, 3 mA) was applied to ipsilateral "Zusanli" (ST 36),"Sanyinjiao" (SP 6) and "Taichong" (LR 3) for 20 min, once daily for 4 weeks. The pathologic changes of the hepatic tissue were detected by H. E. staining. Serum total cholesterol (TC) and triglyceride (TG) contents were determined by using enzymatic methods, serum free fat acids (FFA) content was detected by colorimetry. The expression levels of PPAR-α and L-FABP protein and gene of the liver tissue were determined by Western blot and RT-PCR, respectively. RESULTS: H. E. staining showed that the hepatocytes presented moderate or severe bullous adipose degeneration in rats of the model group, vesicular steatosis in the EA + HFF and LFF groups, turned to almost normal but with small amount of lipid droplets in the EA + LFF group. The contents of serum TC, TG and FFA were significantly higher in the model group than in the control group (P < 0.05), and were obviously decreased in the EA + HFF, LFF and EA + LFF groups in comparison with the model group (P < 0.05). Compared to the control group, hepatic PPAR-α protein and mRNA were markedly down-regulated in the model group, and hepatic L-FABP protein and mRNA considerably up-regulated in the model group (P < 0.05). Following EA intervention and low fat diet feeding, PPAR-α protein and mRNA were markedly up-regulated while L-FABP protein and mRNA remarkably down-regulated in the EA + HFF, LFF and EA + LFF groups (P < 0.05). CONCLUSION: EA intervention or EA combined with dietary control can relieve hepatic pathological changes in NAFLD rats, which may be related to its effects in down-regulating blood lipid level and hepatic L-FABP protein and mRNA expression and in up-regulating PPAR-α protein and mRNA expression, and the effect of combined treatment was relatively better than simple EA or LFF treatment. SN - 1000-0607 UR - https://www.unboundmedicine.com/medline/citation/26669188/[Effects_of_Electroacupunctrue_Combined_with_Dietary_Control_on_Peroxisome_Proliferator_activa__ted_Receptor_α_and_Liver_Fatty_Acid_binding_Protein_Levels_in_Non_alcoholic_Fatty_Liver_Disease_Rats]_ DB - PRIME DP - Unbound Medicine ER -