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[Effect of Electroacupuncture Intervention on Expression of Cx 43 and Concentration of Glutamate in the Striatum of Rats with Parkinson's Disease].
Zhen Ci Yan Jiu. 2015 Oct; 40(5):364-7, 372.ZC

Abstract

OBJECTIVE

To observe the action of electroacupuncture (EA) intervention on the expression of gap junction protein connexin 43 (Cx 43) and content of glutamate (Glu) in the striatum in Parkinson's disease (PD) rats, so as to reveal its mechanism underlying improvement of PD.

METHODS

Forty male SD rats were randomly divided into normal control, sham operation, model and EA groups (n = 10 in each group). The PD model was duplicated by microinjection of 6-hydroxyldopamine (6-OHDA, 15 µg/rat) into the right striatum of rats (AP: 1.0, 1.0; R: 3.0, 4.5; H: 4.5, 6.0), and for control, the same dose of normal saline was injected into the right striatum for rats in the sham operation group. EA (2 Hz, 1 mA) was applied to "Fengfu" (GV 16) "Taichong" (LR 3) for 30 min, once a day for 2 weeks. The PD rats' rotational behavior changes (the numbers of rotations in 30 min) were detected following subcutaneous injection of apomorphine (0.5 mg/kg). The Glu concentration and the expression of Cx 43 in the striatum were detected by using high performance liquid chromatography (HPLC) and Western blot, respectively.

RESULTS

No significant differences were found between the model group and EA group in the number of rotations before the treatment, between the control and sham operation groups in the levels of Glu content and Cx 43 protein expression in the striatum (P > 0.05). Compared with the control group, the Glu content and Cx 43 protein expression level were significantly increased in the model group (P < 0.01), while in comparison with the model group, the number of rotations was significantly reduced in the EA group (P < 0.05). Following EA intervention, both Glu content and Cx 43 expression were considerably down-regulated in the EA group compared with the model group (P < 0.05, P < 0.01).

CONCLUSION

EA can improve PD rats' rotation behavior, which may be associated with its effects in down-regulating the level of Glu and Cx 43 protein expression in the striatum.

Authors

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Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

26669191

Citation

Ma Jun, , et al. "[Effect of Electroacupuncture Intervention On Expression of Cx 43 and Concentration of Glutamate in the Striatum of Rats With Parkinson's Disease]." Zhen Ci Yan Jiu = Acupuncture Research, vol. 40, no. 5, 2015, pp. 364-7, 372.
Ma Jun , Ma B, Wang SJ, et al. [Effect of Electroacupuncture Intervention on Expression of Cx 43 and Concentration of Glutamate in the Striatum of Rats with Parkinson's Disease]. Zhen Ci Yan Jiu. 2015;40(5):364-7, 372.
Ma Jun, ., Ma, B., Wang, S. J., Liu, F., Wang, Y. C., Yu, P. H., & Wang, Z. M. (2015). [Effect of Electroacupuncture Intervention on Expression of Cx 43 and Concentration of Glutamate in the Striatum of Rats with Parkinson's Disease]. Zhen Ci Yan Jiu = Acupuncture Research, 40(5), 364-7, 372.
Ma Jun , et al. [Effect of Electroacupuncture Intervention On Expression of Cx 43 and Concentration of Glutamate in the Striatum of Rats With Parkinson's Disease]. Zhen Ci Yan Jiu. 2015;40(5):364-7, 372. PubMed PMID: 26669191.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effect of Electroacupuncture Intervention on Expression of Cx 43 and Concentration of Glutamate in the Striatum of Rats with Parkinson's Disease]. AU - Ma Jun,, AU - Ma,Biao, AU - Wang,Shu-ju, AU - Liu,Fang, AU - Wang,Yan-chun, AU - Yu,Pei-hao, AU - Wang,Zhong-ming, PY - 2015/12/17/entrez PY - 2015/12/17/pubmed PY - 2016/2/10/medline SP - 364-7, 372 JF - Zhen ci yan jiu = Acupuncture research JO - Zhen Ci Yan Jiu VL - 40 IS - 5 N2 - OBJECTIVE: To observe the action of electroacupuncture (EA) intervention on the expression of gap junction protein connexin 43 (Cx 43) and content of glutamate (Glu) in the striatum in Parkinson's disease (PD) rats, so as to reveal its mechanism underlying improvement of PD. METHODS: Forty male SD rats were randomly divided into normal control, sham operation, model and EA groups (n = 10 in each group). The PD model was duplicated by microinjection of 6-hydroxyldopamine (6-OHDA, 15 µg/rat) into the right striatum of rats (AP: 1.0, 1.0; R: 3.0, 4.5; H: 4.5, 6.0), and for control, the same dose of normal saline was injected into the right striatum for rats in the sham operation group. EA (2 Hz, 1 mA) was applied to "Fengfu" (GV 16) "Taichong" (LR 3) for 30 min, once a day for 2 weeks. The PD rats' rotational behavior changes (the numbers of rotations in 30 min) were detected following subcutaneous injection of apomorphine (0.5 mg/kg). The Glu concentration and the expression of Cx 43 in the striatum were detected by using high performance liquid chromatography (HPLC) and Western blot, respectively. RESULTS: No significant differences were found between the model group and EA group in the number of rotations before the treatment, between the control and sham operation groups in the levels of Glu content and Cx 43 protein expression in the striatum (P > 0.05). Compared with the control group, the Glu content and Cx 43 protein expression level were significantly increased in the model group (P < 0.01), while in comparison with the model group, the number of rotations was significantly reduced in the EA group (P < 0.05). Following EA intervention, both Glu content and Cx 43 expression were considerably down-regulated in the EA group compared with the model group (P < 0.05, P < 0.01). CONCLUSION: EA can improve PD rats' rotation behavior, which may be associated with its effects in down-regulating the level of Glu and Cx 43 protein expression in the striatum. SN - 1000-0607 UR - https://www.unboundmedicine.com/medline/citation/26669191/[Effect_of_Electroacupuncture_Intervention_on_Expression_of_Cx_43_and_Concentration_of_Glutamate_in_the_Striatum_of_Rats_with_Parkinson's_Disease]_ L2 - https://medlineplus.gov/parkinsonsdisease.html DB - PRIME DP - Unbound Medicine ER -