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[Effect of Acupuncture Intervention on Expression of NF-κB Signal Pathway in the Hippocampus of Chronic Stress-induced Depression Rats].
Zhen Ci Yan Jiu. 2015 Oct; 40(5):368-72.ZC

Abstract

OBJECTIVE

To observe the influence of acupuncture intervention on the expression of pivotal pro-inflammatory molecules nuclear factor-κB (NF-κB), cyclooxygenase-2 (COX-2), and prostaglandin E2 (PGE2) of NF-κB inflammatory signal pathway in the hippocampus of depression rats, so as to explore its mechanism underlying improvement of depression.

METHODS

Thirty-six male Sprague-Dawley rats were randomly divided into four groups: control, model, acupuncture, and medication (fluoxetine, Flu) (n = 9 rats in each group). The depression model was established by using chronic unpredictable stress stimuli combined with solitary feeding for 28 days. Acupuncture treatment was applied to "Baihui" (GV 20) and "Neiguan" (PC 6) once every other day for 28 days. Fluoxetine was given to rats of the medication group (10 mg/kg) by gavage (p. o.) everyday for 28 days before the stress stimulation. The expression of NF-κB protein in the hippocampus tissue was detected by Western blot, and the contents of COX-2 and PGE2 were detected by ELISA.

RESULTS

Compared to the control group, the levels of NF-κB protein expression, COX-2 and PGE2 contents in the hippocampus were significantly increased in the model group (P < 0.01). Compared to the model group, the levels of NF-κB protein expression, COX-2 and PGE2 contents in the hippocampus were significantly down-regulated in both the acupuncture and medication groups (P < 0.05; P < 0.01). There was no significant differences between the acupuncture and medication groups in the NF-κB protein expression, and COX-2 and PGE2 contents (P > 0.05).

CONCLUSION

Acupuncture intervention can effectively reduce hippocampal NF-κB expression, and COX-2 and PGE2 levels in depression rats, which may contribute to its anti-depressant effect by inhibiting pro-inflammatory pathway to protect the neurons of hippocampus.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

26669192

Citation

Shao, Run-hui, et al. "[Effect of Acupuncture Intervention On Expression of NF-κB Signal Pathway in the Hippocampus of Chronic Stress-induced Depression Rats]." Zhen Ci Yan Jiu = Acupuncture Research, vol. 40, no. 5, 2015, pp. 368-72.
Shao RH, Jin SY, Lu J, et al. [Effect of Acupuncture Intervention on Expression of NF-κB Signal Pathway in the Hippocampus of Chronic Stress-induced Depression Rats]. Zhen Ci Yan Jiu. 2015;40(5):368-72.
Shao, R. H., Jin, S. Y., Lu, J., Hu, L., & Tu, Y. (2015). [Effect of Acupuncture Intervention on Expression of NF-κB Signal Pathway in the Hippocampus of Chronic Stress-induced Depression Rats]. Zhen Ci Yan Jiu = Acupuncture Research, 40(5), 368-72.
Shao RH, et al. [Effect of Acupuncture Intervention On Expression of NF-κB Signal Pathway in the Hippocampus of Chronic Stress-induced Depression Rats]. Zhen Ci Yan Jiu. 2015;40(5):368-72. PubMed PMID: 26669192.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effect of Acupuncture Intervention on Expression of NF-κB Signal Pathway in the Hippocampus of Chronic Stress-induced Depression Rats]. AU - Shao,Run-hui, AU - Jin,Shu-ying, AU - Lu,Jun, AU - Hu,Li, AU - Tu,Ya, PY - 2015/12/17/entrez PY - 2015/12/17/pubmed PY - 2016/2/10/medline SP - 368 EP - 72 JF - Zhen ci yan jiu = Acupuncture research JO - Zhen Ci Yan Jiu VL - 40 IS - 5 N2 - OBJECTIVE: To observe the influence of acupuncture intervention on the expression of pivotal pro-inflammatory molecules nuclear factor-κB (NF-κB), cyclooxygenase-2 (COX-2), and prostaglandin E2 (PGE2) of NF-κB inflammatory signal pathway in the hippocampus of depression rats, so as to explore its mechanism underlying improvement of depression. METHODS: Thirty-six male Sprague-Dawley rats were randomly divided into four groups: control, model, acupuncture, and medication (fluoxetine, Flu) (n = 9 rats in each group). The depression model was established by using chronic unpredictable stress stimuli combined with solitary feeding for 28 days. Acupuncture treatment was applied to "Baihui" (GV 20) and "Neiguan" (PC 6) once every other day for 28 days. Fluoxetine was given to rats of the medication group (10 mg/kg) by gavage (p. o.) everyday for 28 days before the stress stimulation. The expression of NF-κB protein in the hippocampus tissue was detected by Western blot, and the contents of COX-2 and PGE2 were detected by ELISA. RESULTS: Compared to the control group, the levels of NF-κB protein expression, COX-2 and PGE2 contents in the hippocampus were significantly increased in the model group (P < 0.01). Compared to the model group, the levels of NF-κB protein expression, COX-2 and PGE2 contents in the hippocampus were significantly down-regulated in both the acupuncture and medication groups (P < 0.05; P < 0.01). There was no significant differences between the acupuncture and medication groups in the NF-κB protein expression, and COX-2 and PGE2 contents (P > 0.05). CONCLUSION: Acupuncture intervention can effectively reduce hippocampal NF-κB expression, and COX-2 and PGE2 levels in depression rats, which may contribute to its anti-depressant effect by inhibiting pro-inflammatory pathway to protect the neurons of hippocampus. SN - 1000-0607 UR - https://www.unboundmedicine.com/medline/citation/26669192/[Effect_of_Acupuncture_Intervention_on_Expression_of_NF_κB_Signal_Pathway_in_the_Hippocampus_of_Chronic_Stress_induced_Depression_Rats]_ L2 - http://www.diseaseinfosearch.org/result/2199 DB - PRIME DP - Unbound Medicine ER -