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Generation of Induced Pluripotent Stem Cells From Patients With Duchenne Muscular Dystrophy and Their Induction to Cardiomyocytes.
Int Heart J. 2016; 57(1):112-7.IH

Abstract

Duchenne muscular dystrophy (DMD) is caused by mutations in the DMD gene which encodes dystrophin protein. Dystrophin defect affects cardiac muscle as well as skeletal muscle. Cardiac dysfunction is observed in all patients with DMD over 18 years of age, but there is no curative treatment for DMD cardiomyopathy. To establish novel experimental platforms which reproduce the cardiac phenotype of DMD patients, here we established iPS cell lines from T lymphocytes donated from two DMD patients, with a protocol using Sendai virus vectors. We successfully conducted the differentiation of the DMD patient-specific iPS cells into beating cardiomyocytes. DMD patient-specific iPS cells and iPS cell-derived cardiomyocytes would be a useful in vitro experimental system with which to investigate DMD cardiomyopathy.

Authors+Show Affiliations

Department of Cardiovascular Regenerative Medicine, Osaka University Graduate School of Medicine.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26673445

Citation

Hashimoto, Akihito, et al. "Generation of Induced Pluripotent Stem Cells From Patients With Duchenne Muscular Dystrophy and Their Induction to Cardiomyocytes." International Heart Journal, vol. 57, no. 1, 2016, pp. 112-7.
Hashimoto A, Naito AT, Lee JK, et al. Generation of Induced Pluripotent Stem Cells From Patients With Duchenne Muscular Dystrophy and Their Induction to Cardiomyocytes. Int Heart J. 2016;57(1):112-7.
Hashimoto, A., Naito, A. T., Lee, J. K., Kitazume-Taneike, R., Ito, M., Yamaguchi, T., Nakata, R., Sumida, T., Okada, K., Nakagawa, A., Higo, T., Kuramoto, Y., Sakai, T., Tominaga, K., Okinaga, T., Kogaki, S., Ozono, K., Miyagawa, S., Sawa, Y., ... Komuro, I. (2016). Generation of Induced Pluripotent Stem Cells From Patients With Duchenne Muscular Dystrophy and Their Induction to Cardiomyocytes. International Heart Journal, 57(1), 112-7. https://doi.org/10.1536/ihj.15-376
Hashimoto A, et al. Generation of Induced Pluripotent Stem Cells From Patients With Duchenne Muscular Dystrophy and Their Induction to Cardiomyocytes. Int Heart J. 2016;57(1):112-7. PubMed PMID: 26673445.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Generation of Induced Pluripotent Stem Cells From Patients With Duchenne Muscular Dystrophy and Their Induction to Cardiomyocytes. AU - Hashimoto,Akihito, AU - Naito,Atsuhiko T, AU - Lee,Jong-Kook, AU - Kitazume-Taneike,Rika, AU - Ito,Masamichi, AU - Yamaguchi,Toshihiro, AU - Nakata,Ryo, AU - Sumida,Tomokazu, AU - Okada,Katsuki, AU - Nakagawa,Akito, AU - Higo,Tomoaki, AU - Kuramoto,Yuki, AU - Sakai,Taku, AU - Tominaga,Koji, AU - Okinaga,Takeshi, AU - Kogaki,Shigetoyo, AU - Ozono,Keiichi, AU - Miyagawa,Shigeru, AU - Sawa,Yoshiki, AU - Sakata,Yasushi, AU - Morita,Hiroyuki, AU - Umezawa,Akihiro, AU - Komuro,Issei, Y1 - 2015/12/17/ PY - 2015/12/18/entrez PY - 2015/12/18/pubmed PY - 2016/5/20/medline SP - 112 EP - 7 JF - International heart journal JO - Int Heart J VL - 57 IS - 1 N2 - Duchenne muscular dystrophy (DMD) is caused by mutations in the DMD gene which encodes dystrophin protein. Dystrophin defect affects cardiac muscle as well as skeletal muscle. Cardiac dysfunction is observed in all patients with DMD over 18 years of age, but there is no curative treatment for DMD cardiomyopathy. To establish novel experimental platforms which reproduce the cardiac phenotype of DMD patients, here we established iPS cell lines from T lymphocytes donated from two DMD patients, with a protocol using Sendai virus vectors. We successfully conducted the differentiation of the DMD patient-specific iPS cells into beating cardiomyocytes. DMD patient-specific iPS cells and iPS cell-derived cardiomyocytes would be a useful in vitro experimental system with which to investigate DMD cardiomyopathy. SN - 1349-3299 UR - https://www.unboundmedicine.com/medline/citation/26673445/Generation_of_Induced_Pluripotent_Stem_Cells_From_Patients_With_Duchenne_Muscular_Dystrophy_and_Their_Induction_to_Cardiomyocytes_ L2 - https://dx.doi.org/10.1536/ihj.15-376 DB - PRIME DP - Unbound Medicine ER -