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Cassia obtusifolia seed ameliorates amyloid β-induced synaptic dysfunction through anti-inflammatory and Akt/GSK-3β pathways.
J Ethnopharmacol. 2016 Feb 03; 178:50-7.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Tea infused with the seed of Cassia obtusifolia has been traditionally used as an herbal remedy for liver, eye, and acute inflammatory diseases. Recent pharmacological reports have indicated that Cassiae semen has neuroprotective effects, attributable to its anti-inflammatory actions, in ischemic stroke and Parkinson's disease models.

AIM OF THE STUDY

Previously, the ethanol extract of C. obtusifolia seeds (COE) was reported to have memory enhancing properties. However, the effects of COE in an Alzheimer's disease (AD) model are currently unknown. In this study, we investigated the effect(s) of COE on aberrant synaptic plasticity and memory impairment induced by amyloid β (Aβ), a key toxic component found in the AD brain.

MATERIALS AND METHODS

To determine the effect of COE on Aβ-induced aberrant synaptic plasticity, we used acute mouse hippocampal slices and delivered theta burst stimulation to induce long-term potentiation (LTP). Western blots were used to detect Aβ- and/or COE-induced changes in signaling proteins. The novel object location recognition test was conducted to determine the effect of COE on Aβ-induced recognition memory impairment.

RESULTS

COE was found to ameliorate Aβ-induced LTP impairment in the acute hippocampal slices. Glycogen synthase kinase-3β (GSK-3β), a key molecule in LTP impairment, was activated by Aβ. However, this process was inhibited by COE via Akt signaling. Moreover, COE was found to attenuate Aβ-induced microglia, inducible nitric oxide synthase (iNOS), and cyclooxygenase (COX) activation. In the in vivo studies performed, COE ameliorated the Aβ-induced object recognition memory impairment.

CONCLUSION

These results suggest that COE exhibits neuroprotective activities against Aβ-induced brain disorders.

Authors+Show Affiliations

School of Clinical Sciences, Faculty of Medicine and Dentistry, University of Bristol, Bristol, UK. Electronic address: JeeHyun.Yi@bristol.ac.uk.Department of Medicinal Biotechnology, College of Health Sciences, Dong-A University, Busan 604-714, Republic of Korea; Institute of Convergence Bio-Health, Dong-A University, Busan 604-714, Republic of Korea. Electronic address: qkrgpwls0523@naver.com.Department of Aquatic Biomedical Sciences, School of Marine Biomedical Science, College of Ocean Science, Jeju National University, Jeju 690-756, Republic of Korea. Electronic address: slee76@jejunu.ac.kr.Department of Herbal Medicinal Pharmacology, College of Herbal Bio-industry, Daegu Haany University, Kyungsan 712-715, Republic of Korea. Electronic address: jwjung@dhu.ac.kr.Chonnam-Bristol Frontier Laboratory, Biomedical Research Institute, Chonnam National University Hospital, Jebong-ro, Gwangju 501-757, Republic of Korea. Electronic address: byeong.kim@jnu.ac.kr.Department of Medicinal Biotechnology, College of Health Sciences, Dong-A University, Busan 604-714, Republic of Korea; Institute of Convergence Bio-Health, Dong-A University, Busan 604-714, Republic of Korea. Electronic address: yclee@dau.ac.kr.Department of Life and Nanopharmaceutical Sciences, College of Pharmacy, Kyung Hee University, Seoul 130-701, Republic of Korea; Department of Oriental Pharmaceutical Science, College of Pharmacy, Kyung Hee University, Seoul 130-701, Republic of Korea. Electronic address: jhryu63@khu.ac.kr.Department of Medicinal Biotechnology, College of Health Sciences, Dong-A University, Busan 604-714, Republic of Korea; Institute of Convergence Bio-Health, Dong-A University, Busan 604-714, Republic of Korea. Electronic address: mose79@dau.ac.kr.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26674159

Citation

Yi, Jee Hyun, et al. "Cassia Obtusifolia Seed Ameliorates Amyloid Β-induced Synaptic Dysfunction Through Anti-inflammatory and Akt/GSK-3β Pathways." Journal of Ethnopharmacology, vol. 178, 2016, pp. 50-7.
Yi JH, Park HJ, Lee S, et al. Cassia obtusifolia seed ameliorates amyloid β-induced synaptic dysfunction through anti-inflammatory and Akt/GSK-3β pathways. J Ethnopharmacol. 2016;178:50-7.
Yi, J. H., Park, H. J., Lee, S., Jung, J. W., Kim, B. C., Lee, Y. C., Ryu, J. H., & Kim, D. H. (2016). Cassia obtusifolia seed ameliorates amyloid β-induced synaptic dysfunction through anti-inflammatory and Akt/GSK-3β pathways. Journal of Ethnopharmacology, 178, 50-7. https://doi.org/10.1016/j.jep.2015.12.007
Yi JH, et al. Cassia Obtusifolia Seed Ameliorates Amyloid Β-induced Synaptic Dysfunction Through Anti-inflammatory and Akt/GSK-3β Pathways. J Ethnopharmacol. 2016 Feb 3;178:50-7. PubMed PMID: 26674159.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cassia obtusifolia seed ameliorates amyloid β-induced synaptic dysfunction through anti-inflammatory and Akt/GSK-3β pathways. AU - Yi,Jee Hyun, AU - Park,Hey Jin, AU - Lee,Seungheon, AU - Jung,Ji Wook, AU - Kim,Byeong C, AU - Lee,Young Choon, AU - Ryu,Jong Hoon, AU - Kim,Dong Hyun, Y1 - 2015/12/07/ PY - 2015/06/29/received PY - 2015/10/26/revised PY - 2015/12/02/accepted PY - 2015/12/18/entrez PY - 2015/12/18/pubmed PY - 2016/10/16/medline KW - Amyloid beta KW - Cassia obtusifolia seeds KW - Long-term potentiation KW - Recognition memory SP - 50 EP - 7 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 178 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Tea infused with the seed of Cassia obtusifolia has been traditionally used as an herbal remedy for liver, eye, and acute inflammatory diseases. Recent pharmacological reports have indicated that Cassiae semen has neuroprotective effects, attributable to its anti-inflammatory actions, in ischemic stroke and Parkinson's disease models. AIM OF THE STUDY: Previously, the ethanol extract of C. obtusifolia seeds (COE) was reported to have memory enhancing properties. However, the effects of COE in an Alzheimer's disease (AD) model are currently unknown. In this study, we investigated the effect(s) of COE on aberrant synaptic plasticity and memory impairment induced by amyloid β (Aβ), a key toxic component found in the AD brain. MATERIALS AND METHODS: To determine the effect of COE on Aβ-induced aberrant synaptic plasticity, we used acute mouse hippocampal slices and delivered theta burst stimulation to induce long-term potentiation (LTP). Western blots were used to detect Aβ- and/or COE-induced changes in signaling proteins. The novel object location recognition test was conducted to determine the effect of COE on Aβ-induced recognition memory impairment. RESULTS: COE was found to ameliorate Aβ-induced LTP impairment in the acute hippocampal slices. Glycogen synthase kinase-3β (GSK-3β), a key molecule in LTP impairment, was activated by Aβ. However, this process was inhibited by COE via Akt signaling. Moreover, COE was found to attenuate Aβ-induced microglia, inducible nitric oxide synthase (iNOS), and cyclooxygenase (COX) activation. In the in vivo studies performed, COE ameliorated the Aβ-induced object recognition memory impairment. CONCLUSION: These results suggest that COE exhibits neuroprotective activities against Aβ-induced brain disorders. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/26674159/Cassia_obtusifolia_seed_ameliorates_amyloid_β_induced_synaptic_dysfunction_through_anti_inflammatory_and_Akt/GSK_3β_pathways_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(15)30256-7 DB - PRIME DP - Unbound Medicine ER -