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Enrichment of two isoflavone aglycones in black soymilk by Rhizopus oligosporus NTU 5 in a plastic composite support bioreactor.
J Sci Food Agric. 2016 Aug; 96(11):3779-86.JS

Abstract

BACKGROUND

A plastic composite support (PCS) bioreactor was implemented to evaluate the effects on isoflavone deglycosylation in black soymilk fermented by Rhizopus oligosporus NTU 5.

RESULTS

Evaluation for the optimal PCS for mycelia immobilisation was conducted, which led to the significant results that the most mycelium weight (0.237 g per PCS, P < 0.05) is held by an S-type PCS; therefore, it was selected for black soymilk fermentation. It was found that the PCS fermentation system without pH control exhibits better efficiency of isoflavone bioconversion (daidzin to daidzein, and genistin to genistein) than the one with pH control at pH 6.5. As for the long-run fermentation, those without pH control indeed accelerate the isoflavone bioconversion by continuously releasing β-glucosidase into soymilk. Deglycosylation can be completed in 8 to 24 h and sustained for at least 34 days as 26 batches. The non-pH-control fermentation system also exhibits the highest total phenolic content (ranged from 0.147 to 0.340 mg GAE mL(-1) sample) when compared to the pH-controlled and suspended ones. Meanwhile, the black soymilk from the 22nd batch with 8 h fermentation demonstrated the highest DPPH radical scavenging effect (54.7%).

CONCLUSION

A repeated-batch PCS fermentation system was established to accelerate the deglycosylation rate of isoflavone in black soymilk. © 2015 Society of Chemical Industry.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Liu CT
Institute of Biotechnology, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei, Taiwan.
Erh MH
Graduate Institute of Food Science & Technology, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei, Taiwan.
Lin SP
Institute of Biotechnology, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei, Taiwan.
Lo KY
Department of Agricultural Chemistry, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei, Taiwan.
Chen KI
Graduate Institute of Food Science & Technology, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei, Taiwan.
Cheng KC
Institute of Biotechnology, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei, Taiwan. Graduate Institute of Food Science & Technology, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei, Taiwan.

MeSH

AntioxidantsBiphenyl CompoundsFermentationFood HandlingGenisteinGlycosidesHumansHydrogen-Ion ConcentrationIsoflavonesPicratesRhizopusSoy Milkbeta-Glucosidase

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26676892

Citation

Liu, Chi-Te, et al. "Enrichment of Two Isoflavone Aglycones in Black Soymilk By Rhizopus Oligosporus NTU 5 in a Plastic Composite Support Bioreactor." Journal of the Science of Food and Agriculture, vol. 96, no. 11, 2016, pp. 3779-86.
Liu CT, Erh MH, Lin SP, et al. Enrichment of two isoflavone aglycones in black soymilk by Rhizopus oligosporus NTU 5 in a plastic composite support bioreactor. J Sci Food Agric. 2016;96(11):3779-86.
Liu, C. T., Erh, M. H., Lin, S. P., Lo, K. Y., Chen, K. I., & Cheng, K. C. (2016). Enrichment of two isoflavone aglycones in black soymilk by Rhizopus oligosporus NTU 5 in a plastic composite support bioreactor. Journal of the Science of Food and Agriculture, 96(11), 3779-86. https://doi.org/10.1002/jsfa.7569
Liu CT, et al. Enrichment of Two Isoflavone Aglycones in Black Soymilk By Rhizopus Oligosporus NTU 5 in a Plastic Composite Support Bioreactor. J Sci Food Agric. 2016;96(11):3779-86. PubMed PMID: 26676892.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Enrichment of two isoflavone aglycones in black soymilk by Rhizopus oligosporus NTU 5 in a plastic composite support bioreactor. AU - Liu,Chi-Te, AU - Erh,Mei-Hui, AU - Lin,Shin-Pin, AU - Lo,Kai-Yin, AU - Chen,Kuan-I, AU - Cheng,Kuan-Chen, Y1 - 2016/02/08/ PY - 2015/10/04/received PY - 2015/12/07/revised PY - 2015/12/08/accepted PY - 2015/12/18/entrez PY - 2015/12/18/pubmed PY - 2017/5/11/medline KW - Rhizopus oligosporus KW - black soymilk KW - isoflavone KW - plastic composite support bioreactor KW - β-glucosidase SP - 3779 EP - 86 JF - Journal of the science of food and agriculture JO - J Sci Food Agric VL - 96 IS - 11 N2 - BACKGROUND: A plastic composite support (PCS) bioreactor was implemented to evaluate the effects on isoflavone deglycosylation in black soymilk fermented by Rhizopus oligosporus NTU 5. RESULTS: Evaluation for the optimal PCS for mycelia immobilisation was conducted, which led to the significant results that the most mycelium weight (0.237 g per PCS, P < 0.05) is held by an S-type PCS; therefore, it was selected for black soymilk fermentation. It was found that the PCS fermentation system without pH control exhibits better efficiency of isoflavone bioconversion (daidzin to daidzein, and genistin to genistein) than the one with pH control at pH 6.5. As for the long-run fermentation, those without pH control indeed accelerate the isoflavone bioconversion by continuously releasing β-glucosidase into soymilk. Deglycosylation can be completed in 8 to 24 h and sustained for at least 34 days as 26 batches. The non-pH-control fermentation system also exhibits the highest total phenolic content (ranged from 0.147 to 0.340 mg GAE mL(-1) sample) when compared to the pH-controlled and suspended ones. Meanwhile, the black soymilk from the 22nd batch with 8 h fermentation demonstrated the highest DPPH radical scavenging effect (54.7%). CONCLUSION: A repeated-batch PCS fermentation system was established to accelerate the deglycosylation rate of isoflavone in black soymilk. © 2015 Society of Chemical Industry. SN - 1097-0010 UR - https://www.unboundmedicine.com/medline/citation/26676892/Enrichment_of_two_isoflavone_aglycones_in_black_soymilk_by_Rhizopus_oligosporus_NTU_5_in_a_plastic_composite_support_bioreactor_ DB - PRIME DP - Unbound Medicine ER -