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The involvement of NMDA receptor/NO/cGMP pathway in the antidepressant like effects of baclofen in mouse force swimming test.
Neurosci Lett 2016; 612:52-61NL

Abstract

In the current study, the involvement of N-methyl-d-aspartate receptor (NMDAR) and nitric oxide (NO)/cyclic guanosine monophosphate (cGMP) system in the antidepressant-like effects of baclofen was evaluated by using animal model in forced swimming test. Followed by an open field test for the evaluation of locomotor activity, the immobility time for mice in force swimming test was recorded. Only the last four min was analyzed. Administration of Baclofen (0.5 and 1mg/kg, i.p.) reduced the immobility interval in the FST. Prior administration of l-arginine (750mg/kg, i.p.,) a nitric oxide synthase substrate or sildenafil (5mg/kg, i.p.) a phosphodiesterase 5 into mice suppressed the antidepressant-like activity of baclofen (1mg/kg, i.p.).Co-treatment of 7-nitroindazole (50mg/kg, i.p.,) an inhibitor of neuronal nitric oxide synthase, L-NAME (10mg/kg, i.p.,) a non-specific inhibitor of nitric oxide synthase or MK-801 (0.05mg/kg, i.p.) an NMDA receptor antagonist with subeffective dose of baclofen (0.1mg/kg, i.p.), reduced the immobility time in the FST as compared to the drugs when used alone. Co-administrated of lower doses of MK-801 (0.01mg/kg) or l-NAME (1mg/kg) failed to effect immobility time however, simultaneous administration of these two agents in same dose with subeffective dose of baclofen (0.1mg/kg, i.p.), minimized the immobility time in the FST. Thus, our results support the role of NMDA receptors and l-arginine-NO-GMP pathway in the antidepressant-like action of baclofen.

Authors+Show Affiliations

Experimental Medicine Research Center, Tehran University of Medical Sciences, Tehran, Iran; Department of Pharmacology, School of Medicine, International Campus, Tehran University of Medical Sciences, Tehran, Iran; Department of Neuroscience, School of Advanced Technologies in Medicine, International Campus, Tehran University of Medical Science, Iran.Department of Pharmacology, School of Medicine, International Campus, Tehran University of Medical Sciences, Tehran, Iran; Brain and Spinal Injury Repair Research Center, Imam Khomeini Hospital, Tehran University of Medical Sciences, Tehran, Iran.Department of Tissue Engineering and Applied Cell Sciences Iran University of Medical Sciences, Iran.Experimental Medicine Research Center, Tehran University of Medical Sciences, Tehran, Iran; Department of Pharmacology, School of Medicine, International Campus, Tehran University of Medical Sciences, Tehran, Iran.Department of Neuroscience, School of Advanced Technologies in Medicine, International Campus, Tehran University of Medical Science, Iran.Experimental Medicine Research Center, Tehran University of Medical Sciences, Tehran, Iran; Department of Pharmacology, School of Medicine, International Campus, Tehran University of Medical Sciences, Tehran, Iran; Brain and Spinal Injury Repair Research Center, Imam Khomeini Hospital, Tehran University of Medical Sciences, Tehran, Iran. Electronic address: Dehpour@sina.tums.ac.ir.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26679225

Citation

Khan, Muhammad Imran, et al. "The Involvement of NMDA receptor/NO/cGMP Pathway in the Antidepressant Like Effects of Baclofen in Mouse Force Swimming Test." Neuroscience Letters, vol. 612, 2016, pp. 52-61.
Khan MI, Ostadhadi S, Zolfaghari S, et al. The involvement of NMDA receptor/NO/cGMP pathway in the antidepressant like effects of baclofen in mouse force swimming test. Neurosci Lett. 2016;612:52-61.
Khan, M. I., Ostadhadi, S., Zolfaghari, S., Ejtemaei Mehr, S., Hassanzadeh, G., & Dehpour, A. R. (2016). The involvement of NMDA receptor/NO/cGMP pathway in the antidepressant like effects of baclofen in mouse force swimming test. Neuroscience Letters, 612, pp. 52-61. doi:10.1016/j.neulet.2015.12.006.
Khan MI, et al. The Involvement of NMDA receptor/NO/cGMP Pathway in the Antidepressant Like Effects of Baclofen in Mouse Force Swimming Test. Neurosci Lett. 2016 Jan 26;612:52-61. PubMed PMID: 26679225.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The involvement of NMDA receptor/NO/cGMP pathway in the antidepressant like effects of baclofen in mouse force swimming test. AU - Khan,Muhammad Imran, AU - Ostadhadi,Sattar, AU - Zolfaghari,Samira, AU - Ejtemaei Mehr,Shahram, AU - Hassanzadeh,Gholamreza, AU - Dehpour,Ahmad-Reza, Y1 - 2015/12/08/ PY - 2015/08/19/received PY - 2015/11/18/revised PY - 2015/12/04/accepted PY - 2015/12/19/entrez PY - 2015/12/19/pubmed PY - 2016/6/18/medline KW - Baclofen KW - Depression KW - Forced swimming test KW - Mice KW - N-methyl-d-aspartate (NMDA) KW - Nitric oxide SP - 52 EP - 61 JF - Neuroscience letters JO - Neurosci. Lett. VL - 612 N2 - In the current study, the involvement of N-methyl-d-aspartate receptor (NMDAR) and nitric oxide (NO)/cyclic guanosine monophosphate (cGMP) system in the antidepressant-like effects of baclofen was evaluated by using animal model in forced swimming test. Followed by an open field test for the evaluation of locomotor activity, the immobility time for mice in force swimming test was recorded. Only the last four min was analyzed. Administration of Baclofen (0.5 and 1mg/kg, i.p.) reduced the immobility interval in the FST. Prior administration of l-arginine (750mg/kg, i.p.,) a nitric oxide synthase substrate or sildenafil (5mg/kg, i.p.) a phosphodiesterase 5 into mice suppressed the antidepressant-like activity of baclofen (1mg/kg, i.p.).Co-treatment of 7-nitroindazole (50mg/kg, i.p.,) an inhibitor of neuronal nitric oxide synthase, L-NAME (10mg/kg, i.p.,) a non-specific inhibitor of nitric oxide synthase or MK-801 (0.05mg/kg, i.p.) an NMDA receptor antagonist with subeffective dose of baclofen (0.1mg/kg, i.p.), reduced the immobility time in the FST as compared to the drugs when used alone. Co-administrated of lower doses of MK-801 (0.01mg/kg) or l-NAME (1mg/kg) failed to effect immobility time however, simultaneous administration of these two agents in same dose with subeffective dose of baclofen (0.1mg/kg, i.p.), minimized the immobility time in the FST. Thus, our results support the role of NMDA receptors and l-arginine-NO-GMP pathway in the antidepressant-like action of baclofen. SN - 1872-7972 UR - https://www.unboundmedicine.com/medline/citation/26679225/The_involvement_of_NMDA_receptor/NO/cGMP_pathway_in_the_antidepressant_like_effects_of_baclofen_in_mouse_force_swimming_test_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0304-3940(15)30296-2 DB - PRIME DP - Unbound Medicine ER -