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Changes in Gut and Plasma Microbiome following Exercise Challenge in Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS).
PLoS One. 2015; 10(12):e0145453.Plos

Abstract

Myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) is a disease characterized by intense and debilitating fatigue not due to physical activity that has persisted for at least 6 months, post-exertional malaise, unrefreshing sleep, and accompanied by a number of secondary symptoms, including sore throat, memory and concentration impairment, headache, and muscle/joint pain. In patients with post-exertional malaise, significant worsening of symptoms occurs following physical exertion and exercise challenge serves as a useful method for identifying biomarkers for exertion intolerance. Evidence suggests that intestinal dysbiosis and systemic responses to gut microorganisms may play a role in the symptomology of ME/CFS. As such, we hypothesized that post-exertion worsening of ME/CFS symptoms could be due to increased bacterial translocation from the intestine into the systemic circulation. To test this hypothesis, we collected symptom reports and blood and stool samples from ten clinically characterized ME/CFS patients and ten matched healthy controls before and 15 minutes, 48 hours, and 72 hours after a maximal exercise challenge. Microbiomes of blood and stool samples were examined. Stool sample microbiomes differed between ME/CFS patients and healthy controls in the abundance of several major bacterial phyla. Following maximal exercise challenge, there was an increase in relative abundance of 6 of the 9 major bacterial phyla/genera in ME/CFS patients from baseline to 72 hours post-exercise compared to only 2 of the 9 phyla/genera in controls (p = 0.005). There was also a significant difference in clearance of specific bacterial phyla from blood following exercise with high levels of bacterial sequences maintained at 72 hours post-exercise in ME/CFS patients versus clearance in the controls. These results provide evidence for a systemic effect of an altered gut microbiome in ME/CFS patients compared to controls. Upon exercise challenge, there were significant changes in the abundance of major bacterial phyla in the gut in ME/CFS patients not observed in healthy controls. In addition, compared to controls clearance of bacteria from the blood was delayed in ME/CFS patients following exercise. These findings suggest a role for an altered gut microbiome and increased bacterial translocation following exercise in ME/CFS patients that may account for the profound post-exertional malaise experienced by ME/CFS patients.

Authors+Show Affiliations

Marshfield Clinic Research Foundation, Marshfield, WI, United States of America.William S. Middleton Memorial Veterans Hospital, Madison, WI, United States of America. University of Wisconsin, Madison, WI, United States of America.William S. Middleton Memorial Veterans Hospital, Madison, WI, United States of America.Bateman Horne Center of Excellence, Salt Lake City, UT, United States of America.Marshfield Clinic Research Foundation, Marshfield, WI, United States of America.University of Wisconsin, Madison, WI, United States of America.University of Colorado Denver Anschutz Medical Campus, Aurora, CO, United States of America.Marshfield Clinic Research Foundation, Marshfield, WI, United States of America.Marshfield Clinic, Marshfield, WI, United States of America.University of Colorado Denver Anschutz Medical Campus, Aurora, CO, United States of America.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26683192

Citation

Shukla, Sanjay K., et al. "Changes in Gut and Plasma Microbiome Following Exercise Challenge in Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS)." PloS One, vol. 10, no. 12, 2015, pp. e0145453.
Shukla SK, Cook D, Meyer J, et al. Changes in Gut and Plasma Microbiome following Exercise Challenge in Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS). PLoS One. 2015;10(12):e0145453.
Shukla, S. K., Cook, D., Meyer, J., Vernon, S. D., Le, T., Clevidence, D., Robertson, C. E., Schrodi, S. J., Yale, S., & Frank, D. N. (2015). Changes in Gut and Plasma Microbiome following Exercise Challenge in Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS). PloS One, 10(12), e0145453. https://doi.org/10.1371/journal.pone.0145453
Shukla SK, et al. Changes in Gut and Plasma Microbiome Following Exercise Challenge in Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS). PLoS One. 2015;10(12):e0145453. PubMed PMID: 26683192.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Changes in Gut and Plasma Microbiome following Exercise Challenge in Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS). AU - Shukla,Sanjay K, AU - Cook,Dane, AU - Meyer,Jacob, AU - Vernon,Suzanne D, AU - Le,Thao, AU - Clevidence,Derek, AU - Robertson,Charles E, AU - Schrodi,Steven J, AU - Yale,Steven, AU - Frank,Daniel N, Y1 - 2015/12/18/ PY - 2015/07/13/received PY - 2015/12/03/accepted PY - 2015/12/20/entrez PY - 2015/12/20/pubmed PY - 2016/6/23/medline SP - e0145453 EP - e0145453 JF - PloS one JO - PLoS One VL - 10 IS - 12 N2 - Myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) is a disease characterized by intense and debilitating fatigue not due to physical activity that has persisted for at least 6 months, post-exertional malaise, unrefreshing sleep, and accompanied by a number of secondary symptoms, including sore throat, memory and concentration impairment, headache, and muscle/joint pain. In patients with post-exertional malaise, significant worsening of symptoms occurs following physical exertion and exercise challenge serves as a useful method for identifying biomarkers for exertion intolerance. Evidence suggests that intestinal dysbiosis and systemic responses to gut microorganisms may play a role in the symptomology of ME/CFS. As such, we hypothesized that post-exertion worsening of ME/CFS symptoms could be due to increased bacterial translocation from the intestine into the systemic circulation. To test this hypothesis, we collected symptom reports and blood and stool samples from ten clinically characterized ME/CFS patients and ten matched healthy controls before and 15 minutes, 48 hours, and 72 hours after a maximal exercise challenge. Microbiomes of blood and stool samples were examined. Stool sample microbiomes differed between ME/CFS patients and healthy controls in the abundance of several major bacterial phyla. Following maximal exercise challenge, there was an increase in relative abundance of 6 of the 9 major bacterial phyla/genera in ME/CFS patients from baseline to 72 hours post-exercise compared to only 2 of the 9 phyla/genera in controls (p = 0.005). There was also a significant difference in clearance of specific bacterial phyla from blood following exercise with high levels of bacterial sequences maintained at 72 hours post-exercise in ME/CFS patients versus clearance in the controls. These results provide evidence for a systemic effect of an altered gut microbiome in ME/CFS patients compared to controls. Upon exercise challenge, there were significant changes in the abundance of major bacterial phyla in the gut in ME/CFS patients not observed in healthy controls. In addition, compared to controls clearance of bacteria from the blood was delayed in ME/CFS patients following exercise. These findings suggest a role for an altered gut microbiome and increased bacterial translocation following exercise in ME/CFS patients that may account for the profound post-exertional malaise experienced by ME/CFS patients. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/26683192/Changes_in_Gut_and_Plasma_Microbiome_following_Exercise_Challenge_in_Myalgic_Encephalomyelitis/Chronic_Fatigue_Syndrome__ME/CFS__ L2 - https://dx.plos.org/10.1371/journal.pone.0145453 DB - PRIME DP - Unbound Medicine ER -