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Efficacy of Myrtus communis L. to Inactivate the Hydatid Cyst Protoscoleces.

Abstract

PURPOSE

The present study aims to investigate the scolicidal effects of Myrtus communis L. essential oil against protoscoleces of hydatid cysts and also its toxicity in mice model.

MATERIALS AND METHODS

Protoscoleces were aseptically aspirated from sheep livers having hydatid cysts. Various concentrations of the essential oil (12.5-100 μl/ml) were used for 5-30 min. Viability of protoscoleces was confirmed using eosin exclusion test (0.1% eosin staining). Moreover, 48 male NMRI mice were used to determine the acute and sub-acute toxicity of M. communis essential oil. One-way ANOVA with Tukey's post-hoc test was used to assess differences between experimental groups.

RESULTS

Findings of the present study demonstrated that the M. communis essential oil at the concentration of 100 μl/ml after 5 min of exposure killed 100% protoscoleces. Similarly, the mean mortality rate of protoscoleces after 10 min of exposure to concentration of 50 μl/ml was 100%. However, lower concentrations (12.5 and 25 μl/ml) of M. communis essential oil provoked a delayed protoscolicidal effects. The LD50 values of intraperitoneal injection of the M. communis essential oil was 2.23 mL/kg body wt. No significant difference (p > .05) was observed in the clinical chemistry and hematological parameters following oral administrations of M. communis essential oil at the doses 0.05, 0.1, 0.2, and 0.4 mL/kg for 14 days.

CONCLUSION

The results showed potent scolicidal activity of M. communis with no significant toxicity, which might be used as a natural scolicidal agent in hydatid cyst surgery.

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  • Publisher Full Text
  • Authors+Show Affiliations

    ,

    a Razi Herbal Medicines Research Center , Lorestan University of Medical Sciences , Khorramabad , Islamic Republic of Iran.

    ,

    b Department of Medical Parasitology and Mycology, Faculty of Medicine , Lorestan University of Medical Sciences , Khorramabad , Islamic Republic of Iran.

    ,

    c Department of Surgery , Lorestan University of Medical Sciences , Khorramabad , Islamic Republic of Iran.

    ,

    d Pharmaceutics Research Center, Institute of Neuropharmacology , Kerman University of Medical Sciences , Kerman , Islamic Republic Iran.

    ,

    e Research Center for Hydatid Disease in Iran , University of Medical Sciences , Kerman , Islamic Republic of Iran.

    f Herbal and Traditional Medicines Research Center , Kerman University of Medical Sciences , Kerman , Islamic Republic of Iran.

    Source

    MeSH

    Animals
    Animals, Outbred Strains
    Anti-Infective Agents, Local
    Echinococcosis
    Echinococcus
    Humans
    Injections, Intraperitoneal
    Lethal Dose 50
    Male
    Mice
    Myrtus
    Oils, Volatile
    Plant Oils
    Sheep

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    26684727

    Citation

    Mahmoudvand, Hossein, et al. "Efficacy of Myrtus Communis L. to Inactivate the Hydatid Cyst Protoscoleces." Journal of Investigative Surgery : the Official Journal of the Academy of Surgical Research, vol. 29, no. 3, 2016, pp. 137-43.
    Mahmoudvand H, Fallahi S, Mahmoudvand H, et al. Efficacy of Myrtus communis L. to Inactivate the Hydatid Cyst Protoscoleces. J Invest Surg. 2016;29(3):137-43.
    Mahmoudvand, H., Fallahi, S., Mahmoudvand, H., Shakibaie, M., Harandi, M. F., & Dezaki, E. S. (2016). Efficacy of Myrtus communis L. to Inactivate the Hydatid Cyst Protoscoleces. Journal of Investigative Surgery : the Official Journal of the Academy of Surgical Research, 29(3), pp. 137-43. doi:10.3109/08941939.2015.1088601.
    Mahmoudvand H, et al. Efficacy of Myrtus Communis L. to Inactivate the Hydatid Cyst Protoscoleces. J Invest Surg. 2016;29(3):137-43. PubMed PMID: 26684727.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Efficacy of Myrtus communis L. to Inactivate the Hydatid Cyst Protoscoleces. AU - Mahmoudvand,Hossein, AU - Fallahi,Shirzad, AU - Mahmoudvand,Hormoz, AU - Shakibaie,Mojtaba, AU - Harandi,Majid Fasihi, AU - Dezaki,Ebrahim Saedi, Y1 - 2015/12/18/ PY - 2015/12/20/entrez PY - 2015/12/20/pubmed PY - 2017/5/11/medline KW - cystic echinococcosis KW - hydatid cyst KW - myrtle KW - scolicidal effects KW - toxicity SP - 137 EP - 43 JF - Journal of investigative surgery : the official journal of the Academy of Surgical Research JO - J Invest Surg VL - 29 IS - 3 N2 - PURPOSE: The present study aims to investigate the scolicidal effects of Myrtus communis L. essential oil against protoscoleces of hydatid cysts and also its toxicity in mice model. MATERIALS AND METHODS: Protoscoleces were aseptically aspirated from sheep livers having hydatid cysts. Various concentrations of the essential oil (12.5-100 μl/ml) were used for 5-30 min. Viability of protoscoleces was confirmed using eosin exclusion test (0.1% eosin staining). Moreover, 48 male NMRI mice were used to determine the acute and sub-acute toxicity of M. communis essential oil. One-way ANOVA with Tukey's post-hoc test was used to assess differences between experimental groups. RESULTS: Findings of the present study demonstrated that the M. communis essential oil at the concentration of 100 μl/ml after 5 min of exposure killed 100% protoscoleces. Similarly, the mean mortality rate of protoscoleces after 10 min of exposure to concentration of 50 μl/ml was 100%. However, lower concentrations (12.5 and 25 μl/ml) of M. communis essential oil provoked a delayed protoscolicidal effects. The LD50 values of intraperitoneal injection of the M. communis essential oil was 2.23 mL/kg body wt. No significant difference (p > .05) was observed in the clinical chemistry and hematological parameters following oral administrations of M. communis essential oil at the doses 0.05, 0.1, 0.2, and 0.4 mL/kg for 14 days. CONCLUSION: The results showed potent scolicidal activity of M. communis with no significant toxicity, which might be used as a natural scolicidal agent in hydatid cyst surgery. SN - 1521-0553 UR - https://www.unboundmedicine.com/medline/citation/26684727/Efficacy_of_Myrtus_communis_L__to_Inactivate_the_Hydatid_Cyst_Protoscoleces_ L2 - http://www.tandfonline.com/doi/full/10.3109/08941939.2015.1088601 DB - PRIME DP - Unbound Medicine ER -