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[Establishment and evaluation of the SD rat allergic rhinitis model].

Abstract

OBJECTIVE

To investigate method established and system evaluated in the model of SD rat with AR.

METHOD

To establish AR model of SD rats by ovalbumin (OVA), 20 cases of SD rats were randomly divided into two groups, namely control group (10 cases) and AR group (10 cases). AR models were sensitized and challenged by OVA. Control group were used with normal saline instead of OVA. The score of pathology and praxiology were observed when the SD rats in AR group appeared typical symptom of allergic rhinitis, and levels of IL-4, IFN-γ, IgE in the serum were examined by ELISA. According to the behavioral score, nasal histology and content of IL-4, IFN-γ, IgE of serum, Rat allergic rhinitis model were judged successfully established or not.

RESULT

Behavioral scores were significantly increased in OVA-challenged rats compared with the control group, P<0.05. Nasal epithelial goblet cells, eosinophils and lymphocytes in nasal mucosa in the AR rats exhibited obvious increase relative to the control group. IL-4, IgE levels in the AR rat exhibited obvious increase relative to control group while INF-γ levels exhibited obvious reduction (P<0.05).

CONCLUSION

The allergic rhinitis models in SD rat by OVA were successfully established. The levels of IgE, INF-γ and IL-4 in Serum can be used as objective evaluation of animal models of allergic rhinitis established successfully or not.

Authors

No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

chi

PubMed ID

26685406

Citation

Zhang, Jianhui, and Siquan Tang. "[Establishment and Evaluation of the SD Rat Allergic Rhinitis Model]." Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi = Journal of Clinical Otorhinolaryngology, Head, and Neck Surgery, vol. 29, no. 15, 2015, pp. 1372-4.
Zhang J, Tang S. [Establishment and evaluation of the SD rat allergic rhinitis model]. Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2015;29(15):1372-4.
Zhang, J., & Tang, S. (2015). [Establishment and evaluation of the SD rat allergic rhinitis model]. Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi = Journal of Clinical Otorhinolaryngology, Head, and Neck Surgery, 29(15), pp. 1372-4.
Zhang J, Tang S. [Establishment and Evaluation of the SD Rat Allergic Rhinitis Model]. Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2015;29(15):1372-4. PubMed PMID: 26685406.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Establishment and evaluation of the SD rat allergic rhinitis model]. AU - Zhang,Jianhui, AU - Tang,Siquan, PY - 2015/12/22/entrez PY - 2015/12/22/pubmed PY - 2016/6/17/medline SP - 1372 EP - 4 JF - Lin chuang er bi yan hou tou jing wai ke za zhi = Journal of clinical otorhinolaryngology, head, and neck surgery JO - Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi VL - 29 IS - 15 N2 - OBJECTIVE: To investigate method established and system evaluated in the model of SD rat with AR. METHOD: To establish AR model of SD rats by ovalbumin (OVA), 20 cases of SD rats were randomly divided into two groups, namely control group (10 cases) and AR group (10 cases). AR models were sensitized and challenged by OVA. Control group were used with normal saline instead of OVA. The score of pathology and praxiology were observed when the SD rats in AR group appeared typical symptom of allergic rhinitis, and levels of IL-4, IFN-γ, IgE in the serum were examined by ELISA. According to the behavioral score, nasal histology and content of IL-4, IFN-γ, IgE of serum, Rat allergic rhinitis model were judged successfully established or not. RESULT: Behavioral scores were significantly increased in OVA-challenged rats compared with the control group, P<0.05. Nasal epithelial goblet cells, eosinophils and lymphocytes in nasal mucosa in the AR rats exhibited obvious increase relative to the control group. IL-4, IgE levels in the AR rat exhibited obvious increase relative to control group while INF-γ levels exhibited obvious reduction (P<0.05). CONCLUSION: The allergic rhinitis models in SD rat by OVA were successfully established. The levels of IgE, INF-γ and IL-4 in Serum can be used as objective evaluation of animal models of allergic rhinitis established successfully or not. SN - 1001-1781 UR - https://www.unboundmedicine.com/medline/citation/26685406/[Establishment_and_evaluation_of_the_SD_rat_allergic_rhinitis_model]_ DB - PRIME DP - Unbound Medicine ER -