TY - JOUR T1 - Different zinc sensitivity of Brassica organs is accompanied by distinct responses in protein nitration level and pattern. AU - Feigl,Gábor, AU - Kolbert,Zsuzsanna, AU - Lehotai,Nóra, AU - Molnár,Árpád, AU - Ördög,Attila, AU - Bordé,Ádám, AU - Laskay,Gábor, AU - Erdei,László, Y1 - 2015/12/10/ PY - 2015/08/24/received PY - 2015/11/30/revised PY - 2015/12/03/accepted PY - 2015/12/22/entrez PY - 2015/12/22/pubmed PY - 2016/9/9/medline KW - Brassica juncea KW - Brassica napus KW - Protein tyrosine nitration KW - Reactive nitrogen species KW - Reactive oxygen species KW - Zinc tolerance SP - 141 EP - 52 JF - Ecotoxicology and environmental safety JO - Ecotoxicol Environ Saf VL - 125 N2 - Zinc is an essential microelement, but its excess exerts toxic effects in plants. Heavy metal stress can alter the metabolism of reactive oxygen (ROS) and nitrogen species (RNS) leading to oxidative and nitrosative damages; although the participation of these processes in Zn toxicity and tolerance is not yet known. Therefore this study aimed to evaluate the zinc tolerance of Brassica organs and the putative correspondence of it with protein nitration as a relevant marker for nitrosative stress. Both examined Brassica species (B. juncea and B. napus) proved to be moderate Zn accumulators; however B. napus accumulated more from this metal in its organs. The zinc-induced damages (growth diminution, altered morphology, necrosis, chlorosis, and the decrease of photosynthetic activity) were slighter in the shoot system of B. napus than in B. juncea. The relative zinc tolerance of B. napus shoot was accompanied by moderate changes of the nitration pattern. In contrast, the root system of B. napus suffered more severe damages (growth reduction, altered morphology, viability loss) and slighter increase in nitration level compared to B. juncea. Based on these, the organs of Brassica species reacted differentially to excess zinc, since in the shoot system modification of the nitration pattern occurred (with newly appeared nitrated protein bands), while in the roots, a general increment in the nitroproteome could be observed (the intensification of the same protein bands being present in the control samples). It can be assumed that the significant alteration of nitration pattern is coupled with enhanced zinc sensitivity of the Brassica shoot system and the general intensification of protein nitration in the roots is attached to relative zinc endurance. SN - 1090-2414 UR - https://www.unboundmedicine.com/medline/citation/26685787/Different_zinc_sensitivity_of_Brassica_organs_is_accompanied_by_distinct_responses_in_protein_nitration_level_and_pattern_ DB - PRIME DP - Unbound Medicine ER -