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CYP74B24 is the 13-hydroperoxide lyase involved in biosynthesis of green leaf volatiles in tea (Camellia sinensis).
Plant Physiol Biochem. 2016 Jan; 98:112-8.PP

Abstract

Green leaf volatiles (GLVs) are C6-aliphatic aldehydes/alcohols/acetates, and biosynthesized from the central precursor fatty acid 13-hydroperoxides by 13-hydroperoxide lyases (HPLs) in various plant species. While GLVs have been implicated as defense compounds in plants, GLVs give characteristic grassy note to a bouquet of aroma in green tea, which is manufactured from young leaves of Camellia sinensis. Here we identify three HPL-related genes from C. sinensis via RNA-Sequencing (RNA-Seq) in silico, and functionally characterized a candidate gene, CYP74B24, as a gene encoding tea HPL. Recombinant CYP74B24 protein heterologously expressed in Escherichia coli specifically produced (Z)-3-hexenal from 13-HPOT with the optimal pH 6.0 in vitro. CYP74B24 gene was expressed throughout the aerial organs in a rather constitutive manner and further induced by mechanical wounding. Constitutive expression of CYP74B24 gene in intact tea leaves might account for low but substantial and constitutive formation of a subset of GLVs, some of which are stored as glycosides. Our results not only provide novel insights into the biological roles that GLVs play in tea plants, but also serve as basis for the improvement of aroma quality in tea manufacturing processes.

Authors+Show Affiliations

Research Institute, Suntory Global Innovation Center Ltd, 8-1-1 Seika-dai, Seika, Soraku, Kyoto 619-0238, Japan. Electronic address: eiichiro_ono@suntory.co.jp.Graduate School of Medicine and Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, Yamaguchi 753-8515, Japan. Electronic address: r038ge@yamaguchi-u.ac.jp.Graduate School of Medicine and Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, Yamaguchi 753-8515, Japan. Electronic address: takaori@yamaguchi-u.ac.jp.Research Institute, Suntory Global Innovation Center Ltd, 8-1-1 Seika-dai, Seika, Soraku, Kyoto 619-0238, Japan. Electronic address: hiromi_toyonaga@skal.suntory.co.jp.Graduate School of Medicine and Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, Yamaguchi 753-8515, Japan. Electronic address: t014uj@yamaguchi-u.ac.jp.Bioorganic Research Institute, Suntory Foundation for Life Sciences, 8-1-1 Seika-dai, Seika, Soraku, Kyoto 619-0238, Japan. Electronic address: shiraishi@sunbor.or.jp.Bioorganic Research Institute, Suntory Foundation for Life Sciences, 8-1-1 Seika-dai, Seika, Soraku, Kyoto 619-0238, Japan. Electronic address: murata-j@sunbor.or.jp.Graduate School of Medicine and Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, Yamaguchi 753-8515, Japan. Electronic address: matsui@yamaguchi-u.ac.jp.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26686283

Citation

Ono, Eiichiro, et al. "CYP74B24 Is the 13-hydroperoxide Lyase Involved in Biosynthesis of Green Leaf Volatiles in Tea (Camellia Sinensis)." Plant Physiology and Biochemistry : PPB, vol. 98, 2016, pp. 112-8.
Ono E, Handa T, Koeduka T, et al. CYP74B24 is the 13-hydroperoxide lyase involved in biosynthesis of green leaf volatiles in tea (Camellia sinensis). Plant Physiol Biochem. 2016;98:112-8.
Ono, E., Handa, T., Koeduka, T., Toyonaga, H., Tawfik, M. M., Shiraishi, A., Murata, J., & Matsui, K. (2016). CYP74B24 is the 13-hydroperoxide lyase involved in biosynthesis of green leaf volatiles in tea (Camellia sinensis). Plant Physiology and Biochemistry : PPB, 98, 112-8. https://doi.org/10.1016/j.plaphy.2015.11.016
Ono E, et al. CYP74B24 Is the 13-hydroperoxide Lyase Involved in Biosynthesis of Green Leaf Volatiles in Tea (Camellia Sinensis). Plant Physiol Biochem. 2016;98:112-8. PubMed PMID: 26686283.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - CYP74B24 is the 13-hydroperoxide lyase involved in biosynthesis of green leaf volatiles in tea (Camellia sinensis). AU - Ono,Eiichiro, AU - Handa,Taiki, AU - Koeduka,Takao, AU - Toyonaga,Hiromi, AU - Tawfik,Moataz M, AU - Shiraishi,Akira, AU - Murata,Jun, AU - Matsui,Kenji, Y1 - 2015/11/27/ PY - 2015/09/02/received PY - 2015/11/23/accepted PY - 2015/12/22/entrez PY - 2015/12/22/pubmed PY - 2016/9/28/medline KW - CYP74 KW - Camellia sinensis KW - Green leaf volatile KW - Hydroperoxide lyase KW - Tea SP - 112 EP - 8 JF - Plant physiology and biochemistry : PPB JO - Plant Physiol Biochem VL - 98 N2 - Green leaf volatiles (GLVs) are C6-aliphatic aldehydes/alcohols/acetates, and biosynthesized from the central precursor fatty acid 13-hydroperoxides by 13-hydroperoxide lyases (HPLs) in various plant species. While GLVs have been implicated as defense compounds in plants, GLVs give characteristic grassy note to a bouquet of aroma in green tea, which is manufactured from young leaves of Camellia sinensis. Here we identify three HPL-related genes from C. sinensis via RNA-Sequencing (RNA-Seq) in silico, and functionally characterized a candidate gene, CYP74B24, as a gene encoding tea HPL. Recombinant CYP74B24 protein heterologously expressed in Escherichia coli specifically produced (Z)-3-hexenal from 13-HPOT with the optimal pH 6.0 in vitro. CYP74B24 gene was expressed throughout the aerial organs in a rather constitutive manner and further induced by mechanical wounding. Constitutive expression of CYP74B24 gene in intact tea leaves might account for low but substantial and constitutive formation of a subset of GLVs, some of which are stored as glycosides. Our results not only provide novel insights into the biological roles that GLVs play in tea plants, but also serve as basis for the improvement of aroma quality in tea manufacturing processes. SN - 1873-2690 UR - https://www.unboundmedicine.com/medline/citation/26686283/CYP74B24_is_the_13_hydroperoxide_lyase_involved_in_biosynthesis_of_green_leaf_volatiles_in_tea__Camellia_sinensis__ DB - PRIME DP - Unbound Medicine ER -