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Differential Toll-Like Receptor-Signalling of Burkholderia pseudomallei Lipopolysaccharide in Murine and Human Models.
PLoS One. 2015; 10(12):e0145397.Plos

Abstract

The Gram-negative bacterium Burkholderia pseudomallei causes melioidosis and is a CDC category B bioterrorism agent. Toll-like receptor (TLR)-2 impairs host defense during pulmonary B.pseudomallei infection while TLR4 only has limited impact. We investigated the role of TLRs in B.pseudomallei-lipopolysaccharide (LPS) induced inflammation. Purified B.pseudomallei-LPS activated only TLR2-transfected-HEK-cells during short stimulation but both HEK-TLR2 and HEK-TLR4-cells after 24 h. In human blood, an additive effect of TLR2 on TLR4-mediated signalling induced by B.pseudomallei-LPS was observed. In contrast, murine peritoneal macrophages recognized B.pseudomallei-LPS solely through TLR4. Intranasal inoculation of B.pseudomallei-LPS showed that both TLR4-knockout(-/-) and TLR2x4-/-, but not TLR2-/- mice, displayed diminished cytokine responses and neutrophil influx compared to wild-type controls. These data suggest that B.pseudomallei-LPS signalling occurs solely through murine TLR4, while in human models TLR2 plays an additional role, highlighting important differences between specificity of human and murine models that may have important consequences for B.pseudomallei-LPS sensing by TLRs and subsequent susceptibility to melioidosis.

Authors+Show Affiliations

Center for Infection and Immunity Amsterdam (CINIMA), Academic Medical Center, Amsterdam, the Netherlands. Center for Experimental and Molecular Medicine (CEMM), Academic Medical Center, Amsterdam, the Netherlands.Defence Science and Technology Laboratory, Porton Down, Salisbury, United Kingdom.Center for Infection and Immunity Amsterdam (CINIMA), Academic Medical Center, Amsterdam, the Netherlands. Center for Experimental and Molecular Medicine (CEMM), Academic Medical Center, Amsterdam, the Netherlands.Defence Science and Technology Laboratory, Porton Down, Salisbury, United Kingdom.John Innes Centre, Norwich Research Park, Colney, United Kingdom.John Innes Centre, Norwich Research Park, Colney, United Kingdom.Center for Infection and Immunity Amsterdam (CINIMA), Academic Medical Center, Amsterdam, the Netherlands. Center for Experimental and Molecular Medicine (CEMM), Academic Medical Center, Amsterdam, the Netherlands.Center for Infection and Immunity Amsterdam (CINIMA), Academic Medical Center, Amsterdam, the Netherlands. Center for Experimental and Molecular Medicine (CEMM), Academic Medical Center, Amsterdam, the Netherlands.Center for Infection and Immunity Amsterdam (CINIMA), Academic Medical Center, Amsterdam, the Netherlands. Center for Experimental and Molecular Medicine (CEMM), Academic Medical Center, Amsterdam, the Netherlands.Center for Infection and Immunity Amsterdam (CINIMA), Academic Medical Center, Amsterdam, the Netherlands. Center for Experimental and Molecular Medicine (CEMM), Academic Medical Center, Amsterdam, the Netherlands. Department of Internal Medicine, Division of Infectious Diseases, Academic Medical Center, Amsterdam, the Netherlands.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26689559

Citation

Weehuizen, Tassili A F., et al. "Differential Toll-Like Receptor-Signalling of Burkholderia Pseudomallei Lipopolysaccharide in Murine and Human Models." PloS One, vol. 10, no. 12, 2015, pp. e0145397.
Weehuizen TA, Prior JL, van der Vaart TW, et al. Differential Toll-Like Receptor-Signalling of Burkholderia pseudomallei Lipopolysaccharide in Murine and Human Models. PLoS One. 2015;10(12):e0145397.
Weehuizen, T. A., Prior, J. L., van der Vaart, T. W., Ngugi, S. A., Nepogodiev, S. A., Field, R. A., Kager, L. M., van 't Veer, C., de Vos, A. F., & Wiersinga, W. J. (2015). Differential Toll-Like Receptor-Signalling of Burkholderia pseudomallei Lipopolysaccharide in Murine and Human Models. PloS One, 10(12), e0145397. https://doi.org/10.1371/journal.pone.0145397
Weehuizen TA, et al. Differential Toll-Like Receptor-Signalling of Burkholderia Pseudomallei Lipopolysaccharide in Murine and Human Models. PLoS One. 2015;10(12):e0145397. PubMed PMID: 26689559.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Differential Toll-Like Receptor-Signalling of Burkholderia pseudomallei Lipopolysaccharide in Murine and Human Models. AU - Weehuizen,Tassili A F, AU - Prior,Joann L, AU - van der Vaart,Thomas W, AU - Ngugi,Sarah A, AU - Nepogodiev,Sergey A, AU - Field,Robert A, AU - Kager,Liesbeth M, AU - van 't Veer,Cornelis, AU - de Vos,Alex F, AU - Wiersinga,W Joost, Y1 - 2015/12/21/ PY - 2014/11/20/received PY - 2015/12/03/accepted PY - 2015/12/23/entrez PY - 2015/12/23/pubmed PY - 2016/7/28/medline SP - e0145397 EP - e0145397 JF - PloS one JO - PLoS One VL - 10 IS - 12 N2 - The Gram-negative bacterium Burkholderia pseudomallei causes melioidosis and is a CDC category B bioterrorism agent. Toll-like receptor (TLR)-2 impairs host defense during pulmonary B.pseudomallei infection while TLR4 only has limited impact. We investigated the role of TLRs in B.pseudomallei-lipopolysaccharide (LPS) induced inflammation. Purified B.pseudomallei-LPS activated only TLR2-transfected-HEK-cells during short stimulation but both HEK-TLR2 and HEK-TLR4-cells after 24 h. In human blood, an additive effect of TLR2 on TLR4-mediated signalling induced by B.pseudomallei-LPS was observed. In contrast, murine peritoneal macrophages recognized B.pseudomallei-LPS solely through TLR4. Intranasal inoculation of B.pseudomallei-LPS showed that both TLR4-knockout(-/-) and TLR2x4-/-, but not TLR2-/- mice, displayed diminished cytokine responses and neutrophil influx compared to wild-type controls. These data suggest that B.pseudomallei-LPS signalling occurs solely through murine TLR4, while in human models TLR2 plays an additional role, highlighting important differences between specificity of human and murine models that may have important consequences for B.pseudomallei-LPS sensing by TLRs and subsequent susceptibility to melioidosis. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/26689559/Differential_Toll_Like_Receptor_Signalling_of_Burkholderia_pseudomallei_Lipopolysaccharide_in_Murine_and_Human_Models_ L2 - https://dx.plos.org/10.1371/journal.pone.0145397 DB - PRIME DP - Unbound Medicine ER -