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A microfluidic multiplex proteomic immunoassay device for translational research.
Clin Proteomics. 2015; 12:28.CP

Abstract

OBJECTIVE

Microfluidic technology has the potential to miniaturize and automate complex laboratory procedures. The objective of this study was to assess a microfluidic immunoassay device, Simple Plex, which simultaneously measured IL-1β, TNF-α, IL-6, and IL-10 in serum samples. This assessment is important to understanding the potentials of this microfluidic device as a valuable tool in translational research efforts.

METHODS

We studied the operational characteristics of Simple Plex, and compared to other immunoassay systems including bead-based (i.e., Bio-Plex(®) from Bio-Rad) and planar micro-spot based (i.e., Multi-Array from Meso Scale Discovery) multiplex assays. We determined imprecisions for each of the Simple Plex assays and evaluated the ability of Simple Plex to detect IL-1β, TNF-α, IL-6, and IL-10 in serum samples.

RESULTS

Simple Plex assays required 25 µL serum, and 1.5 h to run 16 samples per cartridge per instrument. Assay imprecisions, evaluated by measurement of 6 replicates in duplicate from a serum pool using three different cartridges, were less than 10 % for all 4 cytokine protein biomarkers, comparable to the imprecisions of traditional ELISAs. The Simple Plex assays were able to detect 32, 95, 97, and 100 % [i.e., percentages of the results within the respective analytical measurement ranges (AMRs)] of IL-1β, TNF-α, IL-6, and IL-10, respectively, in 66 serum samples.

CONCLUSIONS

Simple Plex is a microfluidic multiplex immunoassay device that offers miniaturized, and automated analysis of protein biomarkers. Microfluidic devices such as Simple Plex represent a promising platform to be used in translational research to measure protein biomarkers in real clinical samples.

Authors+Show Affiliations

Department of Laboratory Medicine and Pathology, University of Minnesota, Twin Cities, 420 Delaware Street SE, MMC 609, Minneapolis, MN 55455 USA.Department of Laboratory Medicine and Pathology, University of Minnesota, Twin Cities, 420 Delaware Street SE, MMC 609, Minneapolis, MN 55455 USA.Department of Laboratory Medicine and Pathology, University of Minnesota, Twin Cities, 420 Delaware Street SE, MMC 609, Minneapolis, MN 55455 USA.Department of Laboratory Medicine and Pathology, University of Minnesota, Twin Cities, 420 Delaware Street SE, MMC 609, Minneapolis, MN 55455 USA.Department of Laboratory Medicine and Pathology, University of Minnesota, Twin Cities, 420 Delaware Street SE, MMC 609, Minneapolis, MN 55455 USA.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26692826

Citation

Cao, Jing, et al. "A Microfluidic Multiplex Proteomic Immunoassay Device for Translational Research." Clinical Proteomics, vol. 12, 2015, p. 28.
Cao J, Seegmiller J, Hanson NQ, et al. A microfluidic multiplex proteomic immunoassay device for translational research. Clin Proteomics. 2015;12:28.
Cao, J., Seegmiller, J., Hanson, N. Q., Zaun, C., & Li, D. (2015). A microfluidic multiplex proteomic immunoassay device for translational research. Clinical Proteomics, 12, 28. https://doi.org/10.1186/s12014-015-9101-x
Cao J, et al. A Microfluidic Multiplex Proteomic Immunoassay Device for Translational Research. Clin Proteomics. 2015;12:28. PubMed PMID: 26692826.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A microfluidic multiplex proteomic immunoassay device for translational research. AU - Cao,Jing, AU - Seegmiller,Jesse, AU - Hanson,Naomi Q, AU - Zaun,Christopher, AU - Li,Danni, Y1 - 2015/12/11/ PY - 2015/11/10/received PY - 2015/11/30/accepted PY - 2015/12/23/entrez PY - 2015/12/23/pubmed PY - 2015/12/23/medline KW - Cytokine KW - Immunoassay KW - Microfluidics KW - Multiplex SP - 28 EP - 28 JF - Clinical proteomics JO - Clin Proteomics VL - 12 N2 - OBJECTIVE: Microfluidic technology has the potential to miniaturize and automate complex laboratory procedures. The objective of this study was to assess a microfluidic immunoassay device, Simple Plex, which simultaneously measured IL-1β, TNF-α, IL-6, and IL-10 in serum samples. This assessment is important to understanding the potentials of this microfluidic device as a valuable tool in translational research efforts. METHODS: We studied the operational characteristics of Simple Plex, and compared to other immunoassay systems including bead-based (i.e., Bio-Plex(®) from Bio-Rad) and planar micro-spot based (i.e., Multi-Array from Meso Scale Discovery) multiplex assays. We determined imprecisions for each of the Simple Plex assays and evaluated the ability of Simple Plex to detect IL-1β, TNF-α, IL-6, and IL-10 in serum samples. RESULTS: Simple Plex assays required 25 µL serum, and 1.5 h to run 16 samples per cartridge per instrument. Assay imprecisions, evaluated by measurement of 6 replicates in duplicate from a serum pool using three different cartridges, were less than 10 % for all 4 cytokine protein biomarkers, comparable to the imprecisions of traditional ELISAs. The Simple Plex assays were able to detect 32, 95, 97, and 100 % [i.e., percentages of the results within the respective analytical measurement ranges (AMRs)] of IL-1β, TNF-α, IL-6, and IL-10, respectively, in 66 serum samples. CONCLUSIONS: Simple Plex is a microfluidic multiplex immunoassay device that offers miniaturized, and automated analysis of protein biomarkers. Microfluidic devices such as Simple Plex represent a promising platform to be used in translational research to measure protein biomarkers in real clinical samples. SN - 1542-6416 UR - https://www.unboundmedicine.com/medline/citation/26692826/A_microfluidic_multiplex_proteomic_immunoassay_device_for_translational_research_ L2 - https://clinicalproteomicsjournal.biomedcentral.com/articles/10.1186/s12014-015-9101-x DB - PRIME DP - Unbound Medicine ER -
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