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A Label-Free Impedance Immunosensor Using Screen-Printed Interdigitated Electrodes and Magnetic Nanobeads for the Detection of E. coli O157:H7.
Biosensors (Basel). 2015 Dec 15; 5(4):791-803.B

Abstract

Escherichia coli O157:H7 is one of the leading bacterial pathogens causing foodborne illness. In this study, an impedance immunosensor based on the use of magnetic nanobeads and screen-printed interdigitated electrodes was developed for the rapid detection of E. coli O157:H7. Magnetic nanobeads coated with anti-E. coli antibody were mixed with an E. coli sample and used to isolate and concentrate the bacterial cells. The sample was suspended in redox probe solution and placed onto a screen-printed interdigitated electrode. A magnetic field was applied to concentrate the cells on the surface of the electrode and the impedance was measured. The impedance immunosensor could detect E. coli O157:H7 at a concentration of 10(4.45) cfu·mL(-1) (~1400 bacterial cells in the applied volume of 25 μL) in less than 1 h without pre-enrichment. A linear relationship between bacteria concentration and impedance value was obtained between 10(4.45) cfu·mL(-1) and 10(7) cfu·mL(-1). Though impedance measurement was carried out in the presence of a redox probe, analysis of the equivalent circuit model showed that the impedance change was primarily due to two elements: Double layer capacitance and resistance due to electrode surface roughness. The magnetic field and impedance were simulated using COMSOL Multiphysics software.

Authors+Show Affiliations

Department of Biological and Agricultural Engineering, University of Arkansas, Fayetteville, AR 72701, USA. rwang@uark.edu.Cell and Molecular Biology Program, University of Arkansas, Fayetteville, AR 72701, USA. jlum@pacificvetgroup.com.Department of Biological and Agricultural Engineering, University of Arkansas, Fayetteville, AR 72701, USA. zcallawa@uark.edu.College of Information and Electrical Engineering, China Agricultural University, Beijing 100083, China. jianhan@cau.edu.cn.Center of Excellence for Poultry Science, University of Arkansas, Fayetteville, AR 72701, USA. wbottje@uark.edu.Department of Biological and Agricultural Engineering, University of Arkansas, Fayetteville, AR 72701, USA. yanbinli@uark.edu. Cell and Molecular Biology Program, University of Arkansas, Fayetteville, AR 72701, USA. yanbinli@uark.edu. Center of Excellence for Poultry Science, University of Arkansas, Fayetteville, AR 72701, USA. yanbinli@uark.edu. College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310068, China. yanbinli@uark.edu.

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26694478

Citation

Wang, Ronghui, et al. "A Label-Free Impedance Immunosensor Using Screen-Printed Interdigitated Electrodes and Magnetic Nanobeads for the Detection of E. Coli O157:H7." Biosensors, vol. 5, no. 4, 2015, pp. 791-803.
Wang R, Lum J, Callaway Z, et al. A Label-Free Impedance Immunosensor Using Screen-Printed Interdigitated Electrodes and Magnetic Nanobeads for the Detection of E. coli O157:H7. Biosensors (Basel). 2015;5(4):791-803.
Wang, R., Lum, J., Callaway, Z., Lin, J., Bottje, W., & Li, Y. (2015). A Label-Free Impedance Immunosensor Using Screen-Printed Interdigitated Electrodes and Magnetic Nanobeads for the Detection of E. coli O157:H7. Biosensors, 5(4), 791-803. https://doi.org/10.3390/bios5040791
Wang R, et al. A Label-Free Impedance Immunosensor Using Screen-Printed Interdigitated Electrodes and Magnetic Nanobeads for the Detection of E. Coli O157:H7. Biosensors (Basel). 2015 Dec 15;5(4):791-803. PubMed PMID: 26694478.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A Label-Free Impedance Immunosensor Using Screen-Printed Interdigitated Electrodes and Magnetic Nanobeads for the Detection of E. coli O157:H7. AU - Wang,Ronghui, AU - Lum,Jacob, AU - Callaway,Zach, AU - Lin,Jianhan, AU - Bottje,Walter, AU - Li,Yanbin, Y1 - 2015/12/15/ PY - 2015/10/15/received PY - 2015/12/05/revised PY - 2015/12/11/accepted PY - 2015/12/24/entrez PY - 2015/12/24/pubmed PY - 2016/7/28/medline KW - E. coli O157:H7 KW - immunosensor KW - impedance KW - magnetic nanobeads KW - rapid detection KW - screen-printed interdigitated electrode SP - 791 EP - 803 JF - Biosensors JO - Biosensors (Basel) VL - 5 IS - 4 N2 - Escherichia coli O157:H7 is one of the leading bacterial pathogens causing foodborne illness. In this study, an impedance immunosensor based on the use of magnetic nanobeads and screen-printed interdigitated electrodes was developed for the rapid detection of E. coli O157:H7. Magnetic nanobeads coated with anti-E. coli antibody were mixed with an E. coli sample and used to isolate and concentrate the bacterial cells. The sample was suspended in redox probe solution and placed onto a screen-printed interdigitated electrode. A magnetic field was applied to concentrate the cells on the surface of the electrode and the impedance was measured. The impedance immunosensor could detect E. coli O157:H7 at a concentration of 10(4.45) cfu·mL(-1) (~1400 bacterial cells in the applied volume of 25 μL) in less than 1 h without pre-enrichment. A linear relationship between bacteria concentration and impedance value was obtained between 10(4.45) cfu·mL(-1) and 10(7) cfu·mL(-1). Though impedance measurement was carried out in the presence of a redox probe, analysis of the equivalent circuit model showed that the impedance change was primarily due to two elements: Double layer capacitance and resistance due to electrode surface roughness. The magnetic field and impedance were simulated using COMSOL Multiphysics software. SN - 2079-6374 UR - https://www.unboundmedicine.com/medline/citation/26694478/A_Label_Free_Impedance_Immunosensor_Using_Screen_Printed_Interdigitated_Electrodes_and_Magnetic_Nanobeads_for_the_Detection_of_E__coli_O157:H7_ DB - PRIME DP - Unbound Medicine ER -