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Prevalence of Candida albicans and Candida dubliniensis in caries-free and caries-active children in relation to the oral microbiota-a clinical study.
Clin Oral Investig. 2016 Nov; 20(8):1963-1971.CO

Abstract

OBJECTIVES

The correlation between caries and the oral prevalence of Candida spp. in children is contradictory in literature. Thereby, authors focused on Candida albicans as the most isolated Candida species from the oral cavity. Therefore, the aim of the present study was to compare caries-free and caries-bearing children regarding their oral carriage of Candida spp.

MATERIAL AND METHODS

Twenty-six caries-free (CF group) and 26 caries-active children (CA group) were included into this study. Three different types of specimens were assessed, saliva and plaque, and in the case of caries, infected dentine samples were microbiologically analyzed for aerobic and anaerobic microorganisms and their counts. Special attention was given to the differentiation between C. albicans and Candida dubliniensis. Additionally, different biochemical tests, VITEK 2 (VITEK®2, bioMérieux, Marcy-l'Etoile, France) and 16S and 18S ribosomal DNA (rDNA) sequencing, were applied for identification.

RESULTS

The detection of C. albicans did not differ between the CF and CA groups. C. dubliniensis was never detected in any specimen of the CF group, but occurred in one quarter of the CA group (27 % in plaque, 23 % in saliva), thus leading to a statistically significant difference between the two groups (p < 0.05). In six of these cases, C. dubliniensis was detected concomitantly in saliva and plaque and once only in plaque. CA group harbored statistically more Streptococcus mutans than the control group revealing a correlation between S. mutans and C. dubliniensis regarding the caries group.

CONCLUSIONS

This is the first study reporting a frequent detection of C. dubliniensis in caries-active children, which could have been underestimated so far due to difficulties in differentiation between this yeast species and C. albicans.

CLINICAL RELEVANCE

Microbiological diagnostic-especially of oral Candida species-is an important determinant for identifying etiological factors of dental caries in children.

Authors+Show Affiliations

Department of Operative Dentistry and Periodontology, Center for Dental Medicine, Albert-Ludwigs-University, Freiburg, Germany.Department of Periodontology, Philipps-University, Marburg, Germany.Department of Operative Dentistry and Periodontology, Center for Dental Medicine, Albert-Ludwigs-University, Freiburg, Germany.Institute of Medical Microbiology and Hygiene, Albert-Ludwigs-University, Freiburg, Germany.Institute of Medical Microbiology and Hygiene, Albert-Ludwigs-University, Freiburg, Germany.Department of Statistics, Ludwig-Maximilians-University, Munich, Germany.Department of Operative Dentistry and Periodontology, Center for Dental Medicine, Albert-Ludwigs-University, Freiburg, Germany.Department of Periodontology, Philipps-University, Marburg, Germany. arweiler@med.uni-marburg.de.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26696116

Citation

Al-Ahmad, A, et al. "Prevalence of Candida Albicans and Candida Dubliniensis in Caries-free and Caries-active Children in Relation to the Oral Microbiota-a Clinical Study." Clinical Oral Investigations, vol. 20, no. 8, 2016, pp. 1963-1971.
Al-Ahmad A, Auschill TM, Dakhel R, et al. Prevalence of Candida albicans and Candida dubliniensis in caries-free and caries-active children in relation to the oral microbiota-a clinical study. Clin Oral Investig. 2016;20(8):1963-1971.
Al-Ahmad, A., Auschill, T. M., Dakhel, R., Wittmer, A., Pelz, K., Heumann, C., Hellwig, E., & Arweiler, N. B. (2016). Prevalence of Candida albicans and Candida dubliniensis in caries-free and caries-active children in relation to the oral microbiota-a clinical study. Clinical Oral Investigations, 20(8), 1963-1971.
Al-Ahmad A, et al. Prevalence of Candida Albicans and Candida Dubliniensis in Caries-free and Caries-active Children in Relation to the Oral Microbiota-a Clinical Study. Clin Oral Investig. 2016;20(8):1963-1971. PubMed PMID: 26696116.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Prevalence of Candida albicans and Candida dubliniensis in caries-free and caries-active children in relation to the oral microbiota-a clinical study. AU - Al-Ahmad,A, AU - Auschill,T M, AU - Dakhel,R, AU - Wittmer,A, AU - Pelz,K, AU - Heumann,C, AU - Hellwig,E, AU - Arweiler,N B, Y1 - 2015/12/23/ PY - 2015/06/16/received PY - 2015/12/14/accepted PY - 2016/10/21/pubmed PY - 2017/10/20/medline PY - 2015/12/24/entrez KW - Candida spp. KW - Caries-free and caries-active children KW - Differentiation KW - Prevalence SP - 1963 EP - 1971 JF - Clinical oral investigations JO - Clin Oral Investig VL - 20 IS - 8 N2 - OBJECTIVES: The correlation between caries and the oral prevalence of Candida spp. in children is contradictory in literature. Thereby, authors focused on Candida albicans as the most isolated Candida species from the oral cavity. Therefore, the aim of the present study was to compare caries-free and caries-bearing children regarding their oral carriage of Candida spp. MATERIAL AND METHODS: Twenty-six caries-free (CF group) and 26 caries-active children (CA group) were included into this study. Three different types of specimens were assessed, saliva and plaque, and in the case of caries, infected dentine samples were microbiologically analyzed for aerobic and anaerobic microorganisms and their counts. Special attention was given to the differentiation between C. albicans and Candida dubliniensis. Additionally, different biochemical tests, VITEK 2 (VITEK®2, bioMérieux, Marcy-l'Etoile, France) and 16S and 18S ribosomal DNA (rDNA) sequencing, were applied for identification. RESULTS: The detection of C. albicans did not differ between the CF and CA groups. C. dubliniensis was never detected in any specimen of the CF group, but occurred in one quarter of the CA group (27 % in plaque, 23 % in saliva), thus leading to a statistically significant difference between the two groups (p < 0.05). In six of these cases, C. dubliniensis was detected concomitantly in saliva and plaque and once only in plaque. CA group harbored statistically more Streptococcus mutans than the control group revealing a correlation between S. mutans and C. dubliniensis regarding the caries group. CONCLUSIONS: This is the first study reporting a frequent detection of C. dubliniensis in caries-active children, which could have been underestimated so far due to difficulties in differentiation between this yeast species and C. albicans. CLINICAL RELEVANCE: Microbiological diagnostic-especially of oral Candida species-is an important determinant for identifying etiological factors of dental caries in children. SN - 1436-3771 UR - https://www.unboundmedicine.com/medline/citation/26696116/Prevalence_of_Candida_albicans_and_Candida_dubliniensis_in_caries_free_and_caries_active_children_in_relation_to_the_oral_microbiota_a_clinical_study_ DB - PRIME DP - Unbound Medicine ER -