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Sperm DNA fragmentation and mitochondrial membrane potential combined are better for predicting natural conception than standard sperm parameters.
Fertil Steril 2016; 105(3):637-644.e1FS

Abstract

OBJECTIVE

To evaluate whether DNA fragmentation and/or mitochondrial membrane potential (MMP) predict natural conception better than standard sperm parameters.

DESIGN

Prospective cross-sectional study.

SETTING

University medical center.

PATIENT(S)

Eighty-five infertile and 51 fertile men.

INTERVENTION(S)

Assessment of sperm DNA fragmentation, MMP, and standard semen parameters over a 6- to 12-month observation period.

MAIN OUTCOME MEASURE(S)

Comparison between the results of DNA fragmentation, MMP, and standard sperm parameters alone or combined and achievement of natural conception.

RESULT(S)

Twenty-six of the 85 (31%) men from infertile couples conceived naturally. The median values of DNA fragmentation and MMP in the men who conceived within the observation period were similar to those in the fertile controls. Optimal threshold values of DNA fragmentation and MMP were 25% as determined by receiver operating characteristic analysis (area under the curve [AUC], 0.70; 95% confidence interval (CI) 0.58-0.82) and 62.5% (AUC, 0.68, 95% CI 0.56-0.80), respectively. The men in the infertile group with values of DNA fragmentation ≤25% and with MMP values ≥62.5% had significantly higher odds for conception (odds ratio [OR], 5.22; 95% CI 1.82-14.93] and OR, 4.67; 95% CI 1.74-12.5, respectively). Normal semen analysis alone had no predictive value for natural conception (OR, 1.84; 95% CI 0.67-5.07]). Both sperm function tests combined had significant odds for natural conception (OR, 8.24; 95% CI 2.91-23.33]), with a probability of 0.607 (60.7%) for both normal values and 0.158 (15.8%) for abnormal values.

CONCLUSION(S)

Sperm DNA fragmentation and MMP combined may be superior to standard semen parameters for the prediction of natural conception.

Authors+Show Affiliations

Andrology Unit, Reproductive Unit, Department of Obstetrics and Gynecology, University Medical Center, Ljubljana, Slovenia.Andrology Unit, Reproductive Unit, Department of Obstetrics and Gynecology, University Medical Center, Ljubljana, Slovenia.Institute of Microbiology and Immunology, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia.Institute of Microbiology and Immunology, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia.Andrology Unit, Reproductive Unit, Department of Obstetrics and Gynecology, University Medical Center, Ljubljana, Slovenia.Andrology Unit, Reproductive Unit, Department of Obstetrics and Gynecology, University Medical Center, Ljubljana, Slovenia. Electronic address: branko.zorn@kclj.si.

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

26696300

Citation

Malić Vončina, Slađana, et al. "Sperm DNA Fragmentation and Mitochondrial Membrane Potential Combined Are Better for Predicting Natural Conception Than Standard Sperm Parameters." Fertility and Sterility, vol. 105, no. 3, 2016, pp. 637-644.e1.
Malić Vončina S, Golob B, Ihan A, et al. Sperm DNA fragmentation and mitochondrial membrane potential combined are better for predicting natural conception than standard sperm parameters. Fertil Steril. 2016;105(3):637-644.e1.
Malić Vončina, S., Golob, B., Ihan, A., Kopitar, A. N., Kolbezen, M., & Zorn, B. (2016). Sperm DNA fragmentation and mitochondrial membrane potential combined are better for predicting natural conception than standard sperm parameters. Fertility and Sterility, 105(3), pp. 637-644.e1. doi:10.1016/j.fertnstert.2015.11.037.
Malić Vončina S, et al. Sperm DNA Fragmentation and Mitochondrial Membrane Potential Combined Are Better for Predicting Natural Conception Than Standard Sperm Parameters. Fertil Steril. 2016;105(3):637-644.e1. PubMed PMID: 26696300.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sperm DNA fragmentation and mitochondrial membrane potential combined are better for predicting natural conception than standard sperm parameters. AU - Malić Vončina,Slađana, AU - Golob,Barbara, AU - Ihan,Alojz, AU - Kopitar,Andreja Nataša, AU - Kolbezen,Mojca, AU - Zorn,Branko, Y1 - 2015/12/13/ PY - 2015/07/22/received PY - 2015/11/18/revised PY - 2015/11/19/accepted PY - 2015/12/24/entrez PY - 2015/12/24/pubmed PY - 2016/7/20/medline KW - Male infertility KW - natural conception KW - semen analysis KW - sperm DNA fragmentation KW - sperm mitochondrial membrane potential SP - 637 EP - 644.e1 JF - Fertility and sterility JO - Fertil. Steril. VL - 105 IS - 3 N2 - OBJECTIVE: To evaluate whether DNA fragmentation and/or mitochondrial membrane potential (MMP) predict natural conception better than standard sperm parameters. DESIGN: Prospective cross-sectional study. SETTING: University medical center. PATIENT(S): Eighty-five infertile and 51 fertile men. INTERVENTION(S): Assessment of sperm DNA fragmentation, MMP, and standard semen parameters over a 6- to 12-month observation period. MAIN OUTCOME MEASURE(S): Comparison between the results of DNA fragmentation, MMP, and standard sperm parameters alone or combined and achievement of natural conception. RESULT(S): Twenty-six of the 85 (31%) men from infertile couples conceived naturally. The median values of DNA fragmentation and MMP in the men who conceived within the observation period were similar to those in the fertile controls. Optimal threshold values of DNA fragmentation and MMP were 25% as determined by receiver operating characteristic analysis (area under the curve [AUC], 0.70; 95% confidence interval (CI) 0.58-0.82) and 62.5% (AUC, 0.68, 95% CI 0.56-0.80), respectively. The men in the infertile group with values of DNA fragmentation ≤25% and with MMP values ≥62.5% had significantly higher odds for conception (odds ratio [OR], 5.22; 95% CI 1.82-14.93] and OR, 4.67; 95% CI 1.74-12.5, respectively). Normal semen analysis alone had no predictive value for natural conception (OR, 1.84; 95% CI 0.67-5.07]). Both sperm function tests combined had significant odds for natural conception (OR, 8.24; 95% CI 2.91-23.33]), with a probability of 0.607 (60.7%) for both normal values and 0.158 (15.8%) for abnormal values. CONCLUSION(S): Sperm DNA fragmentation and MMP combined may be superior to standard semen parameters for the prediction of natural conception. SN - 1556-5653 UR - https://www.unboundmedicine.com/medline/citation/26696300/Sperm_DNA_fragmentation_and_mitochondrial_membrane_potential_combined_are_better_for_predicting_natural_conception_than_standard_sperm_parameters_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0015-0282(15)02121-4 DB - PRIME DP - Unbound Medicine ER -