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Surgical removal of endometriotic lesions alters local and systemic proinflammatory cytokines in endometriosis patients.
Fertil Steril. 2016 Apr; 105(4):968-977.e5.FS

Abstract

OBJECTIVE

To determine the impact of endometriotic lesion removal on local and systemic inflammation.

DESIGN

Multiplex cytokine analysis on samples from endometriosis patients before surgery, 2 weeks after surgery, and 3 months after surgery.

SETTING

Academic teaching hospital and university.

PATIENT(S)

A total of 43 endometriosis patients before and after excision of lesions by means of laparoscopic surgery, and 25 normal women.

INTERVENTION(S)

None.

MAIN OUTCOME MEASURE(S)

Plasma, eutopic and ectopic tissue, and peritoneal fluid cytokine levels.

RESULT(S)

Compared with presurgery plasma samples, levels of granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin (IL) 2, IL-8, and IL-10 decreased significantly by 2 weeks after surgery in endometriosis patients. Interestingly, levels began to rise at 3 months after surgery in most cases. In tissue, levels of GM-CSF and IL-15 were lower in eutopic tissue, while levels of basic fibroblast growth factor, interferon-inducible protein 10, IL-1 receptor antagonist, granulocyte colony-stimulating factor, macrophage inflammatory protein 1β, IL-7, and IL-5 were higher in eutopic than in ectopic tissue. In peritoneal fluid, levels of IL-5 and IL-12 were higher in early versus advanced stages of endometriosis. Compared with normal women, plasma from endometriosis patients had higher levels of inflammatory cytokines.

CONCLUSION(S)

Endometriotic lesion removal significantly alters the inflammatory profile both locally and systemically in women with endometriosis. Our findings indicate that ectopic lesions are the major drivers of systemic inflammation in endometriosis. The transitory nature of the change may reflect the recurrence of the condition and the influence of systemic factors in its onset.

Authors+Show Affiliations

Department of Biomedical and Molecular Sciences, Queen's University, Kingston, Ontario, Canada.Department of Biomedical and Molecular Sciences, Queen's University, Kingston, Ontario, Canada.Department of Pathology, Microbiology, and Immunology, University of South Carolina School of Medicine, Columbia, South Carolina.Department of Pathology, Microbiology, and Immunology, University of South Carolina School of Medicine, Columbia, South Carolina.Department of Pathology, Microbiology, and Immunology, University of South Carolina School of Medicine, Columbia, South Carolina.Department of Obstetrics and Gynecology, University of North Carolina, Chapel Hill, North Carolina.Department of Obstetrics and Gynecology, Greenville Health Systems, Greenville, South Carolina.Department of Biomedical and Molecular Sciences, Queen's University, Kingston, Ontario, Canada. Electronic address: tayadec@queensu.ca.

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26698677

Citation

Monsanto, Stephany P., et al. "Surgical Removal of Endometriotic Lesions Alters Local and Systemic Proinflammatory Cytokines in Endometriosis Patients." Fertility and Sterility, vol. 105, no. 4, 2016, pp. 968-977.e5.
Monsanto SP, Edwards AK, Zhou J, et al. Surgical removal of endometriotic lesions alters local and systemic proinflammatory cytokines in endometriosis patients. Fertil Steril. 2016;105(4):968-977.e5.
Monsanto, S. P., Edwards, A. K., Zhou, J., Nagarkatti, P., Nagarkatti, M., Young, S. L., Lessey, B. A., & Tayade, C. (2016). Surgical removal of endometriotic lesions alters local and systemic proinflammatory cytokines in endometriosis patients. Fertility and Sterility, 105(4), 968-e5. https://doi.org/10.1016/j.fertnstert.2015.11.047
Monsanto SP, et al. Surgical Removal of Endometriotic Lesions Alters Local and Systemic Proinflammatory Cytokines in Endometriosis Patients. Fertil Steril. 2016;105(4):968-977.e5. PubMed PMID: 26698677.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Surgical removal of endometriotic lesions alters local and systemic proinflammatory cytokines in endometriosis patients. AU - Monsanto,Stephany P, AU - Edwards,Andrew K, AU - Zhou,Juhua, AU - Nagarkatti,Prakash, AU - Nagarkatti,Mitzi, AU - Young,Steven L, AU - Lessey,Bruce A, AU - Tayade,Chandrakant, Y1 - 2015/12/14/ PY - 2015/09/11/received PY - 2015/11/23/revised PY - 2015/11/23/accepted PY - 2015/12/25/entrez PY - 2015/12/25/pubmed PY - 2016/8/19/medline KW - Cytokines KW - endometriosis KW - inflammation KW - plasma SP - 968 EP - 977.e5 JF - Fertility and sterility JO - Fertil. Steril. VL - 105 IS - 4 N2 - OBJECTIVE: To determine the impact of endometriotic lesion removal on local and systemic inflammation. DESIGN: Multiplex cytokine analysis on samples from endometriosis patients before surgery, 2 weeks after surgery, and 3 months after surgery. SETTING: Academic teaching hospital and university. PATIENT(S): A total of 43 endometriosis patients before and after excision of lesions by means of laparoscopic surgery, and 25 normal women. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): Plasma, eutopic and ectopic tissue, and peritoneal fluid cytokine levels. RESULT(S): Compared with presurgery plasma samples, levels of granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin (IL) 2, IL-8, and IL-10 decreased significantly by 2 weeks after surgery in endometriosis patients. Interestingly, levels began to rise at 3 months after surgery in most cases. In tissue, levels of GM-CSF and IL-15 were lower in eutopic tissue, while levels of basic fibroblast growth factor, interferon-inducible protein 10, IL-1 receptor antagonist, granulocyte colony-stimulating factor, macrophage inflammatory protein 1β, IL-7, and IL-5 were higher in eutopic than in ectopic tissue. In peritoneal fluid, levels of IL-5 and IL-12 were higher in early versus advanced stages of endometriosis. Compared with normal women, plasma from endometriosis patients had higher levels of inflammatory cytokines. CONCLUSION(S): Endometriotic lesion removal significantly alters the inflammatory profile both locally and systemically in women with endometriosis. Our findings indicate that ectopic lesions are the major drivers of systemic inflammation in endometriosis. The transitory nature of the change may reflect the recurrence of the condition and the influence of systemic factors in its onset. SN - 1556-5653 UR - https://www.unboundmedicine.com/medline/citation/26698677/Surgical_removal_of_endometriotic_lesions_alters_local_and_systemic_proinflammatory_cytokines_in_endometriosis_patients_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0015-0282(15)02131-7 DB - PRIME DP - Unbound Medicine ER -