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Presence of Candidatus Neoehrlichia mikurensis and Babesia microti in rodents and two tick species (Ixodes ricinus and Ixodes trianguliceps) in Slovakia.
Ticks Tick Borne Dis. 2016 Mar; 7(2):319-26.TT

Abstract

Rodents are important reservoir hosts of many tick-borne pathogens. Their importance in the circulation of the emerging bacterial agent, Candidatus Neoehrlichia mikurensis and the intraerythrocytic protozoan parasite, Babesia microti has been recently proposed. The aim of the present study was to identify the presence and genetic diversity of Candidatus N. mikurensis and B. microti circulating in the natural foci among rodents and two species of ixodid ticks (Ixodes ricinus and Ixodes trianguliceps). In 2011-2013, rodents were captured at sampling sites in Eastern Slovakia. A total of 997 rodents (324 Apodemus agrarius, 350 Apodemus flavicollis, 271 Myodes glareolus, and 52 other rodent species), 788 feeding ticks from rodents, and 1375 questing ticks were investigated for the presence of pathogens by molecular methods followed by DNA sequencing. Candidatus N. mikurensis was detected in 2.4% of questing I. ricinus nymphs and 2.6% of questing adult I. ricinus ticks, spleens of rodents (1.6%), as well as in feeding larval I. ricinus (0.3%) and feeding larval I. trianguliceps ticks (3.3%). The 16S rRNA and gltA gene sequences of Candidatus N. mikurensis obtained in this study confirmed a high degree of genetic identity of this bacterium in Europe. DNA of B. microti was found in ear (0.6%) and spleen biopsies of rodents (1.9%), in rodent foetus (3.8%) and feeding larval (5.2%) and nymphal (8.7%) I. ricinus, in questing nymphal I. ricinus (0.5%) and questing adult I. ricinus ticks (0.3%). None of the 112 I. trianguliceps ticks were infected. B. microti was represented by two different genotypes: 92% of the positive samples belonged to the zoonotic type strain from Jena (Germany). The results of this study underline the importance of rodents in the circulation of both emerging pathogens in natural foci.

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Authors+Show Affiliations

Blaňarová L
Institute of Parasitology SAS, Hlinkova 3, Košice 040 01, Slovakia. Electronic address: blanarova@saske.sk.
Stanko M
Institute of Parasitology SAS, Hlinkova 3, Košice 040 01, Slovakia; Institute of Zoology SAS, Dúbravská cesta 9, Bratislava 845 06, Slovakia. Electronic address: stankom@saske.sk.
Miklisová D
Institute of Parasitology SAS, Hlinkova 3, Košice 040 01, Slovakia. Electronic address: miklis@saske.sk.
Víchová B
Institute of Parasitology SAS, Hlinkova 3, Košice 040 01, Slovakia. Electronic address: vichova@saske.sk.
Mošanský L
Institute of Parasitology SAS, Hlinkova 3, Košice 040 01, Slovakia. Electronic address: mosansky@saske.sk.
Kraljik J
Institute of Parasitology SAS, Hlinkova 3, Košice 040 01, Slovakia; Department of Zoology, Faculty of Natural Sciences, Comenius University, Mlynská dolina B-1, Bratislava 842 15, Slovakia. Electronic address: kraljik@saske.sk.
Bona M
Department of Anatomy, Faculty of Medicine, Pavol Jozef Šafárik University, Košice, Slovakia. Electronic address: martinbonask@gmail.com.
Derdáková M
Institute of Zoology SAS, Dúbravská cesta 9, Bratislava 845 06, Slovakia. Electronic address: Marketa.Derdakova@gmail.com.

MeSH

AnaplasmataceaeAnaplasmataceae InfectionsAnimalsArachnid VectorsArvicolinaeBabesia microtiBabesiosisDisease ReservoirsFemaleGenetic VariationIxodesMaleMurinaeNymphRNA, Ribosomal, 16SRodentiaSequence Analysis, DNASlovakia

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26700195

Citation

Blaňarová, Lucia, et al. "Presence of Candidatus Neoehrlichia Mikurensis and Babesia Microti in Rodents and Two Tick Species (Ixodes Ricinus and Ixodes Trianguliceps) in Slovakia." Ticks and Tick-borne Diseases, vol. 7, no. 2, 2016, pp. 319-26.
Blaňarová L, Stanko M, Miklisová D, et al. Presence of Candidatus Neoehrlichia mikurensis and Babesia microti in rodents and two tick species (Ixodes ricinus and Ixodes trianguliceps) in Slovakia. Ticks Tick Borne Dis. 2016;7(2):319-26.
Blaňarová, L., Stanko, M., Miklisová, D., Víchová, B., Mošanský, L., Kraljik, J., Bona, M., & Derdáková, M. (2016). Presence of Candidatus Neoehrlichia mikurensis and Babesia microti in rodents and two tick species (Ixodes ricinus and Ixodes trianguliceps) in Slovakia. Ticks and Tick-borne Diseases, 7(2), 319-26. https://doi.org/10.1016/j.ttbdis.2015.11.008
Blaňarová L, et al. Presence of Candidatus Neoehrlichia Mikurensis and Babesia Microti in Rodents and Two Tick Species (Ixodes Ricinus and Ixodes Trianguliceps) in Slovakia. Ticks Tick Borne Dis. 2016;7(2):319-26. PubMed PMID: 26700195.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Presence of Candidatus Neoehrlichia mikurensis and Babesia microti in rodents and two tick species (Ixodes ricinus and Ixodes trianguliceps) in Slovakia. AU - Blaňarová,Lucia, AU - Stanko,Michal, AU - Miklisová,Dana, AU - Víchová,Bronislava, AU - Mošanský,Ladislav, AU - Kraljik,Jasna, AU - Bona,Martin, AU - Derdáková,Markéta, Y1 - 2015/11/26/ PY - 2015/03/03/received PY - 2015/10/31/revised PY - 2015/11/24/accepted PY - 2015/12/25/entrez PY - 2015/12/25/pubmed PY - 2018/2/22/medline KW - Babesia microti KW - Candidatus Neoehrlichia mikurensis KW - Ixodes ricinus KW - Ixodes trianguliceps KW - Rodents KW - Slovakia SP - 319 EP - 26 JF - Ticks and tick-borne diseases JO - Ticks Tick Borne Dis VL - 7 IS - 2 N2 - Rodents are important reservoir hosts of many tick-borne pathogens. Their importance in the circulation of the emerging bacterial agent, Candidatus Neoehrlichia mikurensis and the intraerythrocytic protozoan parasite, Babesia microti has been recently proposed. The aim of the present study was to identify the presence and genetic diversity of Candidatus N. mikurensis and B. microti circulating in the natural foci among rodents and two species of ixodid ticks (Ixodes ricinus and Ixodes trianguliceps). In 2011-2013, rodents were captured at sampling sites in Eastern Slovakia. A total of 997 rodents (324 Apodemus agrarius, 350 Apodemus flavicollis, 271 Myodes glareolus, and 52 other rodent species), 788 feeding ticks from rodents, and 1375 questing ticks were investigated for the presence of pathogens by molecular methods followed by DNA sequencing. Candidatus N. mikurensis was detected in 2.4% of questing I. ricinus nymphs and 2.6% of questing adult I. ricinus ticks, spleens of rodents (1.6%), as well as in feeding larval I. ricinus (0.3%) and feeding larval I. trianguliceps ticks (3.3%). The 16S rRNA and gltA gene sequences of Candidatus N. mikurensis obtained in this study confirmed a high degree of genetic identity of this bacterium in Europe. DNA of B. microti was found in ear (0.6%) and spleen biopsies of rodents (1.9%), in rodent foetus (3.8%) and feeding larval (5.2%) and nymphal (8.7%) I. ricinus, in questing nymphal I. ricinus (0.5%) and questing adult I. ricinus ticks (0.3%). None of the 112 I. trianguliceps ticks were infected. B. microti was represented by two different genotypes: 92% of the positive samples belonged to the zoonotic type strain from Jena (Germany). The results of this study underline the importance of rodents in the circulation of both emerging pathogens in natural foci. SN - 1877-9603 UR - https://www.unboundmedicine.com/medline/citation/26700195/Presence_of_Candidatus_Neoehrlichia_mikurensis_and_Babesia_microti_in_rodents_and_two_tick_species__Ixodes_ricinus_and_Ixodes_trianguliceps__in_Slovakia_ DB - PRIME DP - Unbound Medicine ER -