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Silencing of the gibberellin receptor homolog, CsGID1a, affects locule formation in cucumber (Cucumis sativus) fruit.
New Phytol. 2016 Apr; 210(2):551-63.NP

Abstract

Gibberellins are phytohormones with many roles, including the regulation of fruit development. However, little is known about the relationship between GA perception and fleshy fruit ontogeny, and particularly locule formation. We characterized the expression of cucumber (Cucumis sativus) GA receptor gene (CsGID1a) using quantitative real-time PCR, in situ hybridization and a promoter::β-glucuronidase (GUS) assay. CsGID1a-RNAi cucumber fruits were observed by dissecting microscope, scanning electron microscopy and transmission electron microscopy. Finally, genome-wide gene expression in young fruits from a control and the RNAi line was compared using a digital gene expression (DGE) analysis approach. The expression pattern of CsGID1a was found to be closely correlated with fruit locule formation, and silencing CsGID1a in cucumber resulted in fruits with abnormal carpels and locules. Overexpression of CsGID1a in the Arabidopsis thaliana double mutant (gid1a gid1c) resulted in 'cucumber locule-like' fruits. The DGE analysis suggested that expression of genes related to auxin synthesis and transport, as well as the cell cycle, was altered in CsGID1a-RNAi fruits, a result that was supported by comparing the auxin content and cellular structures of the control and transgenic fruits. This study demonstrates a previously uncharacterized GA signaling pathway that is essential for cucumber fruit locule formation.

Authors+Show Affiliations

Department of Vegetable Science, College of Agronomy and Biotechnology, China Agricultural University, Beijing, 100193, China. Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops of Beijing, China Agricultural University, Beijing, 100193, China.Department of Vegetable Science, College of Agronomy and Biotechnology, China Agricultural University, Beijing, 100193, China. Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops of Beijing, China Agricultural University, Beijing, 100193, China.Department of Vegetable Science, College of Agronomy and Biotechnology, China Agricultural University, Beijing, 100193, China. Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops of Beijing, China Agricultural University, Beijing, 100193, China.Department of Vegetable Science, College of Agronomy and Biotechnology, China Agricultural University, Beijing, 100193, China. Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops of Beijing, China Agricultural University, Beijing, 100193, China.Department of Vegetable Science, College of Agronomy and Biotechnology, China Agricultural University, Beijing, 100193, China. Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops of Beijing, China Agricultural University, Beijing, 100193, China.Department of Vegetable Science, College of Agronomy and Biotechnology, China Agricultural University, Beijing, 100193, China. Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops of Beijing, China Agricultural University, Beijing, 100193, China.Department of Vegetable Science, College of Agronomy and Biotechnology, China Agricultural University, Beijing, 100193, China. Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops of Beijing, China Agricultural University, Beijing, 100193, China.Department of Vegetable Science, College of Agronomy and Biotechnology, China Agricultural University, Beijing, 100193, China. Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops of Beijing, China Agricultural University, Beijing, 100193, China.Department of Vegetable Science, College of Agronomy and Biotechnology, China Agricultural University, Beijing, 100193, China. Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops of Beijing, China Agricultural University, Beijing, 100193, China.Department of Vegetable Science, College of Agronomy and Biotechnology, China Agricultural University, Beijing, 100193, China. Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops of Beijing, China Agricultural University, Beijing, 100193, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26701170

Citation

Liu, Bin, et al. "Silencing of the Gibberellin Receptor Homolog, CsGID1a, Affects Locule Formation in Cucumber (Cucumis Sativus) Fruit." The New Phytologist, vol. 210, no. 2, 2016, pp. 551-63.
Liu B, Liu X, Yang S, et al. Silencing of the gibberellin receptor homolog, CsGID1a, affects locule formation in cucumber (Cucumis sativus) fruit. New Phytol. 2016;210(2):551-63.
Liu, B., Liu, X., Yang, S., Chen, C., Xue, S., Cai, Y., Wang, D., Yin, S., Gai, X., & Ren, H. (2016). Silencing of the gibberellin receptor homolog, CsGID1a, affects locule formation in cucumber (Cucumis sativus) fruit. The New Phytologist, 210(2), 551-63. https://doi.org/10.1111/nph.13801
Liu B, et al. Silencing of the Gibberellin Receptor Homolog, CsGID1a, Affects Locule Formation in Cucumber (Cucumis Sativus) Fruit. New Phytol. 2016;210(2):551-63. PubMed PMID: 26701170.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Silencing of the gibberellin receptor homolog, CsGID1a, affects locule formation in cucumber (Cucumis sativus) fruit. AU - Liu,Bin, AU - Liu,Xingwang, AU - Yang,Sen, AU - Chen,Chunhua, AU - Xue,Shudan, AU - Cai,Yanling, AU - Wang,Dandan, AU - Yin,Shuai, AU - Gai,Xinshuang, AU - Ren,Huazhong, Y1 - 2015/12/24/ PY - 2015/10/08/received PY - 2015/11/11/accepted PY - 2015/12/25/entrez PY - 2015/12/25/pubmed PY - 2016/12/15/medline KW - GA receptor KW - cucumber (Cucumis sativus) KW - digital gene expression (DGE) analysis KW - fleshy fruit KW - locule SP - 551 EP - 63 JF - The New phytologist JO - New Phytol VL - 210 IS - 2 N2 - Gibberellins are phytohormones with many roles, including the regulation of fruit development. However, little is known about the relationship between GA perception and fleshy fruit ontogeny, and particularly locule formation. We characterized the expression of cucumber (Cucumis sativus) GA receptor gene (CsGID1a) using quantitative real-time PCR, in situ hybridization and a promoter::β-glucuronidase (GUS) assay. CsGID1a-RNAi cucumber fruits were observed by dissecting microscope, scanning electron microscopy and transmission electron microscopy. Finally, genome-wide gene expression in young fruits from a control and the RNAi line was compared using a digital gene expression (DGE) analysis approach. The expression pattern of CsGID1a was found to be closely correlated with fruit locule formation, and silencing CsGID1a in cucumber resulted in fruits with abnormal carpels and locules. Overexpression of CsGID1a in the Arabidopsis thaliana double mutant (gid1a gid1c) resulted in 'cucumber locule-like' fruits. The DGE analysis suggested that expression of genes related to auxin synthesis and transport, as well as the cell cycle, was altered in CsGID1a-RNAi fruits, a result that was supported by comparing the auxin content and cellular structures of the control and transgenic fruits. This study demonstrates a previously uncharacterized GA signaling pathway that is essential for cucumber fruit locule formation. SN - 1469-8137 UR - https://www.unboundmedicine.com/medline/citation/26701170/Silencing_of_the_gibberellin_receptor_homolog_CsGID1a_affects_locule_formation_in_cucumber__Cucumis_sativus__fruit_ L2 - https://doi.org/10.1111/nph.13801 DB - PRIME DP - Unbound Medicine ER -