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Silver nanoparticles induced neurotoxicity through oxidative stress in rat cerebral astrocytes is distinct from the effects of silver ions.
Neurotoxicology. 2016 Jan; 52:210-21.N

Abstract

The rapid development of silver nanoparticles (AgNPs) based products has raised increasing concerns in view of their potential hazardous risks to the environment and human health. The roles of the released silver ions in AgNPs induced cytotoxicities are being hotly debated. Using rat cerebral astrocytes, the neurotoxicological effects of AgNPs and silver ions were investigated. Acute toxicity based on Alamar Blue assay showed that silver ions were considerably more toxic than AgNPs. Comparative studies indicated that AgNPs increased caspase activities and induced cell apoptosis under cytotoxic level of exposures, while silver ions compromised cell membrane integrity and dominantly caused cell necrosis. Cellular internalization of silver provided the basis for the cytotoxicities of these two silver species. In contrast to silver ions, intracellular reactive oxygen species (ROS) generation occurred in time- and concentration-dependent manners in astrocytes upon AgNPs stimulation, which caused subsequent c-Jun N-terminal kinases (JNK) phosphorylation and promoted the programmed cell death. Non-cytotoxic level of AgNPs exposure increased multiple cytokines secretion from the astrocytes, indicating that AgNPs were potentially involved in neuroinflammation. This effect was independent of silver ions as well. The distinct toxicological effects caused by AgNPs and silver ions provided the solid proofs for the particle-specific effects which should be concerned regarding the accurate assessment of AgNPs exposure risks.

Authors+Show Affiliations

State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, PR China.State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, PR China.State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, PR China; School of Earth and Space Science, University of Science and Technology of China, Anhui 230026, PR China.State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, PR China; Institute of Chemical Safety, Chinese Academy of Inspection and Quarantine, Beijing 100176, PR China.Center for Biological Imaging, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, PR China.State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, PR China.State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, PR China. Electronic address: zhouqf@rcees.ac.cn.State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, PR China.

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26702581

Citation

Sun, Cheng, et al. "Silver Nanoparticles Induced Neurotoxicity Through Oxidative Stress in Rat Cerebral Astrocytes Is Distinct From the Effects of Silver Ions." Neurotoxicology, vol. 52, 2016, pp. 210-21.
Sun C, Yin N, Wen R, et al. Silver nanoparticles induced neurotoxicity through oxidative stress in rat cerebral astrocytes is distinct from the effects of silver ions. Neurotoxicology. 2016;52:210-21.
Sun, C., Yin, N., Wen, R., Liu, W., Jia, Y., Hu, L., Zhou, Q., & Jiang, G. (2016). Silver nanoparticles induced neurotoxicity through oxidative stress in rat cerebral astrocytes is distinct from the effects of silver ions. Neurotoxicology, 52, 210-21. https://doi.org/10.1016/j.neuro.2015.09.007
Sun C, et al. Silver Nanoparticles Induced Neurotoxicity Through Oxidative Stress in Rat Cerebral Astrocytes Is Distinct From the Effects of Silver Ions. Neurotoxicology. 2016;52:210-21. PubMed PMID: 26702581.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Silver nanoparticles induced neurotoxicity through oxidative stress in rat cerebral astrocytes is distinct from the effects of silver ions. AU - Sun,Cheng, AU - Yin,Nuoya, AU - Wen,Ruoxi, AU - Liu,Wei, AU - Jia,Yanxia, AU - Hu,Ligang, AU - Zhou,Qunfang, AU - Jiang,Guibin, Y1 - 2015/12/15/ PY - 2015/06/09/received PY - 2015/09/06/revised PY - 2015/09/14/accepted PY - 2015/12/26/entrez PY - 2015/12/26/pubmed PY - 2016/12/15/medline KW - Astrocyte KW - Neurotoxicity KW - Particle effect KW - Silver ion release KW - Silver nanoparticle SP - 210 EP - 21 JF - Neurotoxicology JO - Neurotoxicology VL - 52 N2 - The rapid development of silver nanoparticles (AgNPs) based products has raised increasing concerns in view of their potential hazardous risks to the environment and human health. The roles of the released silver ions in AgNPs induced cytotoxicities are being hotly debated. Using rat cerebral astrocytes, the neurotoxicological effects of AgNPs and silver ions were investigated. Acute toxicity based on Alamar Blue assay showed that silver ions were considerably more toxic than AgNPs. Comparative studies indicated that AgNPs increased caspase activities and induced cell apoptosis under cytotoxic level of exposures, while silver ions compromised cell membrane integrity and dominantly caused cell necrosis. Cellular internalization of silver provided the basis for the cytotoxicities of these two silver species. In contrast to silver ions, intracellular reactive oxygen species (ROS) generation occurred in time- and concentration-dependent manners in astrocytes upon AgNPs stimulation, which caused subsequent c-Jun N-terminal kinases (JNK) phosphorylation and promoted the programmed cell death. Non-cytotoxic level of AgNPs exposure increased multiple cytokines secretion from the astrocytes, indicating that AgNPs were potentially involved in neuroinflammation. This effect was independent of silver ions as well. The distinct toxicological effects caused by AgNPs and silver ions provided the solid proofs for the particle-specific effects which should be concerned regarding the accurate assessment of AgNPs exposure risks. SN - 1872-9711 UR - https://www.unboundmedicine.com/medline/citation/26702581/Silver_nanoparticles_induced_neurotoxicity_through_oxidative_stress_in_rat_cerebral_astrocytes_is_distinct_from_the_effects_of_silver_ions_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0161-813X(15)00136-9 DB - PRIME DP - Unbound Medicine ER -