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Cytochrome b5 Reductase 1 Triggers Serial Reactions that Lead to Iron Uptake in Plants.
Mol Plant. 2016 Apr 04; 9(4):501-13.MP

Abstract

Rhizosphere acidification is essential for iron (Fe) uptake into plant roots. Plasma membrane (PM) H(+)-ATPases play key roles in rhizosphere acidification. However, it is not fully understood how PM H(+)-ATPase activity is regulated to enhance root Fe uptake under Fe-deficient conditions. Here, we present evidence that cytochrome b5 reductase 1 (CBR1) increases the levels of unsaturated fatty acids, which stimulate PM H(+)-ATPase activity and thus lead to rhizosphere acidification. CBR1-overexpressing (CBR1-OX) Arabidopsis thaliana plants had higher levels of unsaturated fatty acids (18:2 and 18:3), higher PM H(+)-ATPase activity, and lower rhizosphere pH than wild-type plants. By contrast, cbr1 loss-of-function mutant plants showed lower levels of unsaturated fatty acids and lower PM H(+)-ATPase activity but higher rhizosphere pH. Reduced PM H(+)-ATPase activity in cbr1 could be restored in vitro by addition of unsaturated fatty acids. Transcript levels of CBR1, fatty acids desaturase2 (FAD2), and fatty acids desaturase3 (FAD3) were increased under Fe-deficient conditions. We propose that CBR1 has a crucial role in increasing the levels of unsaturated fatty acids, which activate the PM H(+)-ATPase and thus reduce rhizosphere pH. This reaction cascade ultimately promotes root Fe uptake.

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Authors+Show Affiliations

Oh YJ
Division of Integrative Biosciences and Biotechnology, Pohang University of Science and Technology, Pohang 790-784, Korea.
Kim H
Department Life Sciences, Pohang University of Science and Technology, Pohang 790-784, Korea.
Seo SH
Division of Environmental Science and Engineering, Pohang University of Science and Technology, Pohang 790-784, Korea.
Hwang BG
Division of Mechanical Engineering, Pohang University of Science and Technology, Pohang 790-784, Korea.
Chang YS
Division of Environmental Science and Engineering, Pohang University of Science and Technology, Pohang 790-784, Korea.
Lee J
Department Life Sciences, Pohang University of Science and Technology, Pohang 790-784, Korea.
Lee DW
Division of Integrative Biosciences and Biotechnology, Pohang University of Science and Technology, Pohang 790-784, Korea.
Sohn EJ
Division of Integrative Biosciences and Biotechnology, Pohang University of Science and Technology, Pohang 790-784, Korea.
Lee SJ
Division of Mechanical Engineering, Pohang University of Science and Technology, Pohang 790-784, Korea.
Lee Y
Division of Integrative Biosciences and Biotechnology, Pohang University of Science and Technology, Pohang 790-784, Korea; Department Life Sciences, Pohang University of Science and Technology, Pohang 790-784, Korea.
Hwang I
Division of Integrative Biosciences and Biotechnology, Pohang University of Science and Technology, Pohang 790-784, Korea; Department Life Sciences, Pohang University of Science and Technology, Pohang 790-784, Korea. Electronic address: ihhwang@postech.ac.kr.

MeSH

ArabidopsisArabidopsis ProteinsBiological TransportCell MembraneCytochrome-B(5) ReductaseExtracellular SpaceFatty Acids, UnsaturatedGene Expression Regulation, PlantIronMutationProton-Translocating ATPasesRhizosphereSeedsSoilSolubilityUp-Regulation

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26712506

Citation

Oh, Young Jun, et al. "Cytochrome B5 Reductase 1 Triggers Serial Reactions That Lead to Iron Uptake in Plants." Molecular Plant, vol. 9, no. 4, 2016, pp. 501-13.
Oh YJ, Kim H, Seo SH, et al. Cytochrome b5 Reductase 1 Triggers Serial Reactions that Lead to Iron Uptake in Plants. Mol Plant. 2016;9(4):501-13.
Oh, Y. J., Kim, H., Seo, S. H., Hwang, B. G., Chang, Y. S., Lee, J., Lee, D. W., Sohn, E. J., Lee, S. J., Lee, Y., & Hwang, I. (2016). Cytochrome b5 Reductase 1 Triggers Serial Reactions that Lead to Iron Uptake in Plants. Molecular Plant, 9(4), 501-13. https://doi.org/10.1016/j.molp.2015.12.010
Oh YJ, et al. Cytochrome B5 Reductase 1 Triggers Serial Reactions That Lead to Iron Uptake in Plants. Mol Plant. 2016 Apr 4;9(4):501-13. PubMed PMID: 26712506.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cytochrome b5 Reductase 1 Triggers Serial Reactions that Lead to Iron Uptake in Plants. AU - Oh,Young Jun, AU - Kim,Hanul, AU - Seo,Sung Hee, AU - Hwang,Bae Geun, AU - Chang,Yoon Seok, AU - Lee,Junho, AU - Lee,Dong Wook, AU - Sohn,Eun Ju, AU - Lee,Sang Joon, AU - Lee,Youngsook, AU - Hwang,Inhwan, Y1 - 2015/12/19/ PY - 2015/09/29/received PY - 2015/11/23/revised PY - 2015/12/07/accepted PY - 2015/12/30/entrez PY - 2015/12/30/pubmed PY - 2017/1/17/medline KW - H(+)-ATPase KW - cytochrome b5 reductase 1 (CBR1) KW - iron (Fe) uptake KW - unsaturated fatty acids SP - 501 EP - 13 JF - Molecular plant JO - Mol Plant VL - 9 IS - 4 N2 - Rhizosphere acidification is essential for iron (Fe) uptake into plant roots. Plasma membrane (PM) H(+)-ATPases play key roles in rhizosphere acidification. However, it is not fully understood how PM H(+)-ATPase activity is regulated to enhance root Fe uptake under Fe-deficient conditions. Here, we present evidence that cytochrome b5 reductase 1 (CBR1) increases the levels of unsaturated fatty acids, which stimulate PM H(+)-ATPase activity and thus lead to rhizosphere acidification. CBR1-overexpressing (CBR1-OX) Arabidopsis thaliana plants had higher levels of unsaturated fatty acids (18:2 and 18:3), higher PM H(+)-ATPase activity, and lower rhizosphere pH than wild-type plants. By contrast, cbr1 loss-of-function mutant plants showed lower levels of unsaturated fatty acids and lower PM H(+)-ATPase activity but higher rhizosphere pH. Reduced PM H(+)-ATPase activity in cbr1 could be restored in vitro by addition of unsaturated fatty acids. Transcript levels of CBR1, fatty acids desaturase2 (FAD2), and fatty acids desaturase3 (FAD3) were increased under Fe-deficient conditions. We propose that CBR1 has a crucial role in increasing the levels of unsaturated fatty acids, which activate the PM H(+)-ATPase and thus reduce rhizosphere pH. This reaction cascade ultimately promotes root Fe uptake. SN - 1752-9867 UR - https://www.unboundmedicine.com/medline/citation/26712506/Cytochrome_b5_Reductase_1_Triggers_Serial_Reactions_that_Lead_to_Iron_Uptake_in_Plants_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1674-2052(15)00463-3 DB - PRIME DP - Unbound Medicine ER -